The effect of HSP47 antisense to protect the pulmonary fibrosis caused by paraquat intoxication

百草枯是一种有争议的除草剂，也是世界上最常用的除草剂之一。虽然肝脏、肾脏、心脏和中枢神经系统受到影响，但肺损伤导致肺纤维化是中毒病例中常见的死亡原因。百草枯的毒性机制尚不清楚，但我们发现了热休克蛋白47（Hsp47）的诱导，胶原特异性分子伴侣，与肺纤维化有关。Hsp47反义，对治疗纤维化有有益作用，可提高百草枯处理大鼠模型的存活率。Hsp47反义蛋白的抗纤维化作用是由于抑制胶原纤维的折叠、组装和运输。

Paraquat is a controversial and one of the most commonly used herbicides in the world. Although liver, kidney, heart and CNS are affected, lung damage resulting to pulmonary fibrosis is the usual cause of deaths in the cases with intoxication. The mechanism of paraquat toxicity is not clear but we showed the induction of heat shock protein 47(Hsp47), collagen specific molecular chaperone, related to pulmonary fibrosis. Hsp47 antisense, has beneficial effects on the treatment of fibrosis, and increased survival rate of paraquat treated rat model. The antifibrotic effect of Hsp47 antisense has shown to be due to inhibition of folding, assembly and transport of collagen fibers.