Studies on the potential of the dental mesenchyme for the dental regeneration

牙齿间充质对牙齿再生潜力的研究

• 批准号: 16591840
• 负责人: YAMAMOTO Hitoshi
• 金额: $ 2.24万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591840/
• 关键词: tooth germ; epithelial-mesenchymal interaction; regeneration; organ culture; transplantation; gene expression; ultrastructure; reaggregation system; マウス

Tooth morphogenesis is generally considered to be a good model system for epithelial-mesenchyme interaction. The dental epithelium has the potential to induce the tooth formation prior to the bud stage in mice. However, this potential shifts later from the dental epithelium to the dental mesenchyme. To clarify the essential properties of the dental mesenchyme, reaggregation experiments were carried out. The results showed that the reaggregated mesenchyme could form tooth and periodontium. Then, tissue recombination experiments were performed to identify the basis for the potential to induce tooth formation. Recombination between the dental epithelium of the diastema and the dental mesenchyme of the tooth germ resulted in tooth formation, whereas although recombination between the dental epithelium of the tooth germ and the dental mesenchyme of the diastema resulted in failure to form a tooth. Since the mesenchymal cells did not condense around the diastema bud, dental mesenchyme has the ability to induce tooth formation as its essential properties, whereas it is necessary to condensation surround the tooth bud. In addition, to analyze the effects of transplantation, the enamel of a transplanted tooth was observed by scanning electron microscopy. Although the enamel was divided into three layers by the running pattern of the enamel prisms as in normal teeth, the width of each layer was different from that of a normal tooth. It seems likely that the microenvironment may play a role in forming the enamel structure, since the tooth germs grow inside of calcified bone.

牙齿形态发生通常被认为是上皮-间充质相互作用的良好模型系统。牙上皮细胞具有诱导小鼠牙胚形成的潜能。然而，这种潜力转移后，从牙齿上皮细胞的牙齿间充质。为了阐明牙齿间充质的基本性质，进行了再聚集实验。结果表明，重新聚集的间充质可形成牙齿和牙周组织。然后，进行组织重组实验以确定诱导牙齿形成的潜力的基础。牙间隙的牙上皮和牙胚的牙间充质之间的重组导致牙齿形成，而牙胚的牙上皮和牙间隙的牙间充质之间的重组导致牙齿形成失败。由于间充质细胞不凝聚在牙间隙芽周围，因此牙间充质具有诱导牙齿形成的能力，而牙间隙芽周围的凝聚是必要的。此外，为了分析移植的效果，通过扫描电子显微镜观察移植牙的釉质。虽然像正常牙齿一样，釉质被釉质棱柱的运行模式分为三层，但每层的宽度与正常牙齿的宽度不同。似乎微环境可能在形成釉质结构中起作用，因为牙胚生长在钙化的骨骼内。

项目成果

On the dentine structure evolution with the correlation of enamel

论牙本质结构演化与牙釉质的相关性

• 发表时间: 2005
• 期刊: Journal of Comparative Development of Tooth Enamel 9
• 作者: Yukishige Kozawa;Tetsuo Terajima;Kunihiro Suzuki;Hitoshi Yamamoto;Rumi Yokota;Hideki Chisaka;Kazumasa Hoshino;Tomohisa Honda
• 通讯作者: Tomohisa Honda

The epithelial mesenchymal interaction for tooth morphogenesis with special reference to the arrest of normal tooth development

牙齿形态发生的上皮间充质相互作用，特别是正常牙齿发育的停滞

• 发表时间: 2007
• 期刊: Current Topic in Genetics (印刷中)
• 作者: Soeno;Y.;Hitoshi Yamamoto
• 通讯作者: Hitoshi Yamamoto

象牙質の進化についての一考察 : エナメル質との関連性において

牙本质进化的研究：与牙釉质的关系

• 发表时间: 2005
• 期刊: エナメル質比較発生学懇話会記録 9
• 作者: Fujitake T;Taya Y;Uchikawa Y;Aoba T;小澤幸重
• 通讯作者: 小澤幸重

Development of ameloblasts grouping and dancing in Indian elephant molar tooth germ

印度象臼齿胚成釉细胞群和舞蹈的发育

• 发表时间: 2005
• 期刊: Current Trends in Dental Morphology Research
• 作者: Takaaki Aoba(Editor) (Edited by Dept.of Pathology;The Nippon Dental Univ.);日本歯科大学病理学講座編;Takashi Muramatsu;Hitoshi Yamamoto;Yukishige Kozawa
• 通讯作者: Yukishige Kozawa

歯の再生

牙齿再生

• 发表时间: 2006
• 作者: Aoki;H. et al.;山崎英俊
• 通讯作者: 山崎英俊