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The water metabolism dysfunction of inner ear on the occurrence of endolymphatic hydrops

内耳水代谢功能障碍与内淋巴水肿发生的关系

基本信息

批准号：
14571616
负责人：
SAKURAI Hironori
金额：
$ 2.24万
依托单位：
Shiga university of medical science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571616/
关键词：
endolymphatic sac inner ear viral infection Meniere's disease varicella-zoster virus Epstein-Barr virus Polymerase chain reaction method in situ hybridization 内リンパ水腫帯状疱疹ウィルス PCR メニエール病ウイルス in situ hybridization

项目摘要

The main purpose of this is to search for a viral etiology in Meniere's disease by examining the presence or absence of herpes family virus DNA in the endolymphatic sac (ES) using the in situ hybridization method. This was a prospective study with the ES from 10 patients with Meniere's disease and from 7 control cases without any pre-mortem ear disease except a case of acoustic tumor. These 10 patients underwent the ES surgery. The presence of herpes family virus DNA, such as herpes simplex virus type 1 and 2 (HSV1 and 2), varicella-zoster virus (VZV), Estein-Barr virus (EVB) and human cytomegalovirus (CMV), was examined using the in situ hybridization method. Serum antibody titers against these viruses just before the ES surgery were studied in these patients. Of the 10 specimens from the patients with Meniere's disease, 7 were positive for VZV, 4 for EBV, one for CMV and none for HSV 1 and 2, although the serum antibody titers against these viruses did not show any significant elevation in these patients just before the ES surgery. This result suggests that the viral DNA in the ES is inactive and is present in a latent form. From the statistical analysis, it can be postulated that VZV infection in early childhood may reach the ES and play a role in the pathogenesis of Meniere's disease (p=0.0235). The double infection with both VZV and EBV tended to be another candidate for the pathogenesis of Meniere's disease (p=0.0557).

本研究的主要目的是通过使用原位杂交方法检查内淋巴囊（ES）中疱疹病毒家族DNA的存在或不存在来寻找梅尼埃病的病毒病因。这是一项前瞻性研究，ES来自10例梅尼埃病患者和7例对照病例，除1例听肿瘤外，无任何生前耳部疾病。这10例患者接受了ES手术。采用原位杂交方法检测单纯疱疹病毒1型和2型（HSV 1和2）、水痘-带状疱疹病毒（VZV）、EVB和人巨细胞病毒（CMV）等疱疹病毒家族DNA的存在。在这些患者中研究了ES手术前针对这些病毒的血清抗体滴度。梅尼埃病患者的10份标本中，7份为VZV阳性，4份为EBV阳性，1份为CMV阳性，无1份为HSV 1和2阳性，尽管在ES手术前这些患者的血清抗这些病毒的抗体滴度未显示任何显著升高。该结果表明ES中的病毒DNA是无活性的，并且以潜伏形式存在。从统计学分析，可以推测，VZV感染在幼儿期可能达到ES，并在梅尼埃病的发病机制中发挥作用（p=0.0235）。VZV和EBV的双重感染倾向于成为梅尼埃病发病机制的另一候选者（p=0.0557）。