Suppression of angiogenesis in oral cancer by cyclooxygenase inhibitors

环氧合酶抑制剂抑制口腔癌的血管生成

• 批准号: 14571893
• 负责人: KOIZUMI Koichi
• 金额: $ 2.24万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571893/
• 关键词: VEGF; oral cancer; NSAIDs; COX-2

It is well known that cyclooxygenase-2(COX-2) induces prostaglandin E2 (PGE2) synthesis and facilitates angiogenesis. Because synthesis of vascular endothelial growth factor (VEGF) is regulated by PGE2, COX-2 also might induce VEGF synthesis. We have investigated the expression of COX-2 mRNA in oral squamous cell carcinoma (SCC) cells, KA, NA, NI, KO and UE, and adenocarcinoma cells of salivary grand, HSG and HSY by RT-PCR. Any cells except UE expressed COX-2 mRNA. Nonsteroidal anti inflammatory drugs (NSAIDs), which inhibit cyclooxygenase enzyme activity, suppressed the growth of oral SCC cells expressing COX-2 mRNA. However, NSAIDs showed no effect on the growth UE cells lacking the expression of COX-2 mRNA. These findings suggest that NSAIDS, COX-2-Inhibitor inhibits angiogenesis by suppression of VEGF synthesis as well as proliferation

环氧化酶-2（COX-2）可诱导前列腺素E2 （PGE2）合成并促进血管生成。由于血管内皮生长因子（VEGF）的合成受PGE2的调控，COX-2也可能诱导VEGF的合成。我们采用RT-PCR方法研究了COX-2 mRNA在口腔鳞状细胞癌（SCC）细胞、KA、NA、NI、KO和UE以及大涎腺癌细胞、HSG和HSY中的表达。除UE外，其余细胞均表达COX-2 mRNA。抑制环氧化酶活性的非甾体抗炎药（NSAIDs）抑制了表达COX-2 mRNA的口腔SCC细胞的生长。然而，非甾体抗炎药对缺乏COX-2 mRNA表达的UE细胞的生长没有影响。这些发现表明，非甾体抗炎药cox -2抑制剂通过抑制VEGF合成和增殖来抑制血管生成

项目成果

Hayashido, Y.: "Role of stromal thrombospondin-1 in motility and proteolytic activity of oral squamous cell carcinoma cells."International Journal of Molecular Medicine. 12(4). 447-452 (2003)

Hayashido, Y.：“基质血小板反应蛋白-1 在口腔鳞状细胞癌细胞的运动和蛋白水解活性中的作用。”国际分子医学杂志。

Hayashido, Y: "Role of stromal thrombospondin-1 in motility and proteolytic activity of oral squamous cell carcinoma cells"International Journal of Molecular Medicine. 12(4). 447-452 (2003)

Hayashido, Y：“基质血小板反应蛋白-1 在口腔鳞状细胞癌细胞运动和蛋白水解活性中的作用”国际分子医学杂志。

北野尚孝: "口腔扁平上皮癌細胞の浸潤増殖におけるインテグリンαvの関与"組織培養学会誌. 11(1). 11-18 (2002)

Naotaka Kitano：“整合素αv参与口腔鳞状细胞癌细胞的侵袭性生长”组织培养学会杂志11（1）（2002）。

Hisataka, Kitano: "Participation of integrin αa v in invasive growth of oral squamous cell carcinoma cells"Jpn.J.Tissue Cult.Dent.Res. 11(1). 11-18 (2002)

Hisataka，Kitano：“整合素αa v参与口腔鳞状细胞癌细胞的侵袭性生长”Jpn.J.Tissue Cult.Dent.Res 11(1)(2002)。