Fabrication of bioresorbable porous ceramics for bone regeneration and its bone reconstruction using transplantation with the blood vessel pattern

用于骨再生的生物可吸收多孔陶瓷的制备及其利用血管模型移植的骨重建

• 批准号: 14571894
• 负责人: MIYAMOTO Youji
• 金额: $ 2.18万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571894/
• 关键词: calcium phpsphate; porous ceramics; hydroxypatite; bone marrow cell; carbonate apatite; biometerials; リン酸カルシウムセラミックス; 連続気孔; 培養骨髄細胞

The purposes of this study are (1) to fabricate the porous ceramics for bone regeneration using biodegradable calcium phosphate and (2) to evaluate bone regeneration by the ceramics and cultured bone marrow cells in vivo and in vitro.Porous biodegradable calcium phosphate ceramics were fabricated by following methods : the polyurethane foams with different pore size were impregnated with hydroxyapatite or carbonate apatite solution. By the centrifugal operation, the specimens dried, after the surplus slurry was removed, and they were fired. On a part of the specimens, the impregnation, drying, and firing process was repeated. The specimens were observed by scanning electron microscopy. As the result, the trabecula of the ceramics thickened, and the pore size decreased with the increase of the cycles. This result indicates that average diameter of the ceramic trabecula, diameter of the pore and porosity of the ceramics could be adjusted by the number of the cycles.Rat marrow cells were cultured for 2 weeks with in medium supplemented with ascorbic acid, beta-glycerophosphate, and dexamethasone. Cell proliferation, alkaline phosphatase activity, type I collagen synthesis, and osteocalcin production were measured, and the osteogenesis in vitro was evaluated. As the result, the osteogenesis in vitro was promoted with the increase in the pore size under 100 _m, however, the pore size over 100_m, did not affect the osteogenesis.The ceramics/marrow cell complex cultured for 2 weeks were implanted into 7-week-old syngeneic rats. The osteogenesis in vivo was histologically evaluated at 8 weeks after the implantation. As the result, pore sizes between 100 to 200 U m were optimum. However, the absorption of ceramics in this period was slight. Further studies of the long-term resorption of the ceramics and stability of the bone will be needed.

本研究的目的是(1)利用可生物降解的磷酸钙制备可用于骨再生的多孔陶瓷；(2)评价该陶瓷和培养的骨髓细胞在体内和体外的骨再生能力。通过离心操作，样品干燥，剩余的浆料被去除后，它们被烧制。在部分样品上，重复浸渍、干燥和烧制过程。对标本进行扫描电子显微镜观察。结果表明，随着循环次数的增加，陶瓷的骨小梁变厚，孔径减小。这一结果表明，陶瓷的平均直径、孔径和孔隙率可以根据循环次数进行调整。大鼠骨髓细胞在添加抗坏血酸、β-甘油磷酸和地塞米松的培养液中培养2周。检测细胞增殖、碱性磷酸酶活性、I型胶原合成和骨钙素生成，并评价体外成骨能力。结果表明，在100m以下，随着孔径的增大，体外成骨能力增强，而当孔径大于100m时，对成骨无影响。将培养2周的陶瓷/骨髓细胞复合体植入7周龄同基因大鼠体内。植入后8周取材，进行体内成骨组织学检测。结果表明，孔径在100~200um之间为最佳。然而，这一时期对陶瓷的吸收很少。陶瓷的长期吸收和骨的稳定性还需要进一步的研究。

项目成果

M.Takechi, Y.Miyamoto et al.: "Effects of Various Sterilization Methods on the Setting and Mechanical Properties of Apatite Cement."Journal of Biomedical Materials Research. Vol.69(No.1). 58-63 (2004)

M.Takechi、Y.Miyamoto 等人：“各种灭菌方法对磷灰石水泥凝固和机械性能的影响”。生物医学材料研究杂志。

M.TAKECHI(他6名): "Effects of Various Sterilization Methods on the Setting and Mechanical Properties of Apatite Cement"Journal of Biomedical Material Research. 69(1). 58-63 (2004)

M.TAKECHI（其他 6 名）：“各种灭菌方法对磷灰石水泥凝固和机械性能的影响”生物医学材料研究杂志 69（1）58-63（2004）。

Y.MOMOTA(他6名): "Effects of Neutral Sodium Hydrogen Phosphate on the Setting Property and Hemostatic Ability of Hydroxyapatite Putty as a Local Hemostatic Agent for Bone"Journal of Biomedical Material Research. 69(1). 99-103 (2004)

Y.MOMOTA（其他6人）：“中性​​磷酸氢钠对骨局部止血剂羟基磷灰石凝固性能和止血能力的影响”生物医学材料研究杂志69（1）（2004）。

Y.Momota, Y.Miyamoto et al.: "Effects of Neutral Sodium Hydrogen Phosphate on the Setting Property and Hemostatic Ability of Hydroxyapatite Putty as a Local Hemostatic Agent for Bone."Journal of Biomedical Materials Research. Vol.69(No.1). 99-103 (2004)

Y.Momota，Y.Miyamoto 等人：“中性磷酸氢钠对作为骨局部止血剂的羟基磷灰石腻子的凝固性能和止血能力的影响。”生物医学材料研究杂志。