Fabrication of scaffolds made of bioresorbable polymer for bone and cartilage regeneration and its bone reconstruction using transplantation with the blood vessel pattern.

制造由生物可吸收聚合物制成的支架，用于骨和软骨再生以及使用血管模型移植进行骨重建。

• 批准号: 16592003
• 负责人: MIYAMOTO Youji
• 金额: $ 2.3万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16592003/
• 关键词: biomaterial; polymer; scaffold; hydroxyapatite; regenerative medicine; carbonate apatite; bone; bioresorbability; スキャホールド

The purposes of this study are (1) to fabricate scaffold with bioresorbable polymer for bone and cartilage regeneration and (2) to evaluate bone regeneration by the bioresorbable polymer scaffold and cultured bone marrow cells in vivo.Porous bioresorbable polymer scaffold was fabricated by solvent-casting particulate leaching methods : after poly(D,L-lactide-co-glycolic acid)(PLGA)(75/25) was dissolved in dimethyl sulfoxide, the solution was poured into a mold filled up with the various sizes (0.6, 1.0, 2.0 mm) of sucrose crystals. Then, the mold was transferred to a fridge at -18℃ for 1h to set the mixture. The PLGA was precipitated and the sugar crystals were leached out of the precipitated PLGA in distilled water. The specimens were observed by scanning electron microscopy. As the result, the PLGA madoreporite (PLGA) had continuous pores, and the pore size increased with the crystal size of sucrose. This result indicates that average diameter of diameter of the pore and porosity of … More PLGA could be adjusted by the crystal size. We also fabricated porous bioresorbable polymer scaffold (CHAp-PLGA) with osteoconductivity by mixing low crystalline carbonate apatite particles into PLGA (75/25).The rat bone marrow cells were seeded into CHAp-PLGA (CHAp-PLGA with cells) and, then cultured in medium supplemented with ascorbic acid, beta-glycerophosphate, and dexamethasone for 2 weeks. PLGA, CHAp-PLGA and CHAp-PLGA with cells were implanted into 7-week-old syngeneic rats. The osteogenesis in vivo was histologically evaluated at 3, 8 weeks after the implantation. Although no bone formation was observed in PLGA, new bone formation was seen in CHAp-PLGA and CHAp-PLGA with cells. In the case of CHAp-PLGA with cells, the larger amount of bone was formed more rapidly than CHAp-PLGA without cells.As a conclusion, CHAp-PLGA seemed to become a promising alternative of the scaffold for bone and cartilage regeneration. Further studies of the long-term resorption of CHAp-PLGA and stability of the formed bone will be needed. Less

本研究的目的是（1）制备用于骨和软骨再生的生物可吸收聚合物支架，（2）评价生物可吸收聚合物支架和培养的骨髓细胞在体内的骨再生。将聚（D，L-丙交酯-共-乙醇酸）（PLGA）（75/25）溶解在二甲基亚砜中后，将溶液倒入填充有各种尺寸（0.6、1.0、2.0mm）的蔗糖晶体的模具中。然后，将模具转移到-18 ° C的冰箱中1小时以使混合物凝固。使PLGA沉淀，并在蒸馏水中从沉淀的PLGA中浸出糖晶体。采用扫描电子显微镜对标本进行观察。结果，PLGA钙钛矿（PLGA）具有连续的孔，且孔径随着蔗糖晶体尺寸的增大而增大。这一结果表明，孔隙直径的平均直径和孔隙率 ...更多信息 PLGA可以通过晶体尺寸进行调节。我们还将低结晶碳酸磷灰石颗粒混入PLGA（75/25）中，制备了具有骨传导性的多孔生物可吸收聚合物支架（CHAp-PLGA）。将大鼠骨髓细胞接种到CHAp-PLGA（CHAp-PLGA+细胞）中，然后在添加抗坏血酸、β-甘油磷酸盐和地塞米松的培养基中培养2周。将PLGA、CHAp-PLGA和CHAp-PLGA与细胞一起植入7周龄的同基因大鼠中。分别于植入后3、8周行组织学观察。虽然在PLGA中没有观察到骨形成，但在CHAp-PLGA和含细胞的CHAp-PLGA中观察到新骨形成。CHAp-PLGA在含细胞的情况下，比不含细胞的CHAp-PLGA更快地形成更大量的骨，因此CHAp-PLGA似乎成为骨和软骨再生支架的有希望的替代物。需要进一步研究CHAp-PLGA的长期再吸收和形成骨的稳定性。少

项目成果

A basic research on hydroxyapatite-poly (D,L-lactide-cc-glycolic acid) composite scaffolds for bone tissue engineering.

骨组织工程用羟基磷灰石-聚(D,L-丙交酯-α-乙醇酸)复合支架的基础研究。

• 发表时间: 2005
• 期刊: Archives of BioCeramics Research 5・1
• 作者: Minamiguchi S.;Takechi M.;Yuasa T.;Momota Y.;Tatehara S.;Miyamoto Y.;Satomura K.;Nagayama M.
• 通讯作者: Nagayama M.

A basic research on hydroxyapatite -poly (D,L-lactide-cc-glycolic acid) composite scaffolds for bone tissue engineering.

羟基磷灰石-聚(D,L-丙交酯-α-乙醇酸)复合骨组织工程支架的基础研究

• 发表时间: 2005
• 期刊: Archives of BioCeramics Research 5(1)
• 作者: Minamiguchi S.;Takechi M.;Yuasa T.;Momota Y;Tatehara S.;Miyamoto Y;Satomura K.;Nagayama M.
• 通讯作者: Nagayama M.

A basic research on hydroxyapatite-poly (D, L-lactide-cc-glycolic acid) composite scaffolds for bone tissue engineering

羟基磷灰石-聚(D,L-丙交酯-α-乙醇酸)复合骨组织工程支架的基础研究

• 发表时间: 2005
• 期刊: Archives of BioCeramics Research 5・1
• 作者: Minamiguchi S.;Takeshi M.;Yuasa T.;Momota Y.;Tatehara S.;Miyamoto Y.;Satomura K.;Nagayama M.
• 通讯作者: Nagayama M.