Establisment of differentiation method from induced pluripotent stem(iPS) cells to osteoblasts via mesenchymal stem cells

本研究的目的是建立一种诱导iPS细胞向间充质干细胞分化的方法，并通过加入TGF-β和视黄酸来提高iPS细胞向成骨细胞的分化。然后，我们通过加入骨诱导培养基来检测间充质干细胞向成骨细胞的分化。成骨细胞分化标志物表达增强。此外，当间充质干细胞在生物陶瓷如羟基磷灰石上培养时，这些成骨细胞分化标志物的表达也增加。因此，证明了iPS细胞可以通过间充质干细胞分化为成骨细胞。

The aim of this study is to establish a method to differentiate iPS cells into mesenchymal stem cells, followed by to differentiate mesenchymal stem cells into osteoblasts, in order to efficiently differentiate from iPS cells into osteoblasts.The expressions of the markers of mesenchymal stem cells, were enhanced by addition of TGF-βand retinoic acid. Then, we examined the differentiation of the mesenchymal stem cells into osteoblasts by addition of bone induced medium. The expressions of the differentiation markers of osteoblasts were enhanced. In addition, it was revealed that the expression of these differentiation markers of osteoblasts was also increased when the mesenchymal stem cells were cultured on bioceramics, such as hydroxyapatite. Therefore, it was demonstrated that the iPS cells can differentiate into osteoblasts via the mesenchymal stem cells.