The in vivo and in vitro studies for the effect of mechanical loads on the temporomandibular joint disc

机械载荷对颞下颌关节盘影响的体内外研究

• 批准号: 14571950
• 负责人: TANAKA Eiji
• 金额: $ 2.56万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571950/
• 关键词: Temporomandibular joint disc; Viscoelastic property; Lubrication; Cultured disc cell; Mechanical load; Joint friction; Hyaluronic acid; PDGF; 培養細胞; 関節摩擦

Information about the viscoelastic behavior of the temporomandibular joint (TMJ) disc is required for its function to be understood and, for instance, for its tissue engineering. The aim of this research was, thus, to examine the dynamic viscoelastic properties of the TMJ discs and the lubricating ability in the TMJ. Using the porcine TMJ discs, the dynamic viscoelastic properties in tension, compression and shear were examined. The dynamic moduli increased as the loading frequency increased. Of the components constructing TMJ disc, the proteoglycans play an important role in determining the viscoelastic properties of the disc and, therefore, give the disc a greater capacity for distributing and reducing stresses. With respect to the TMJ lubrication, we conducted the first investigation for clarifying the lubricating ability of the intact TMJ. The mean frictional coefficient of porcine intact TMJ was 0.0145 after a constant load of 50 N during 5 s. The frictional coefficient increased with the length of the preceding load duration and 0.022 after 1 hr. Furthermore, the reduced lubricating ability by OA-like structure could be restored by the addition of hyaluronic acid with high molecular weight to a certain extent. Considering these findings, we started to do the tissue engineering, of the TMJ disc. Considering the composition of extracellular matrix and the cell types, Platelet derived growth factor (PDGF) may be effective for TMJ disc cell proliferation and metabolism. Thus, we investigate the effect of additive PDGF on the TMJ disc cell proliferation and synthesis of extracellular matrix. As the result, high doses of PDGF had the major effects on cultured disc cell proliferation, collagen and hyaluronic acid syntheses. This implies that the administration of PDGF may have future therapeutic potential in the treatment of disc degeneration, especially in tissue engineering of TMJ disc

颞下颌关节（TMJ）盘的粘弹性行为的信息是需要了解其功能，例如，其组织工程。本研究的目的是，因此，检查的动态粘弹性能的颞下颌关节盘和润滑能力的颞下颌关节。利用猪颞下颌关节盘，研究了其拉伸、压缩和剪切的动态粘弹特性。动态模量随加载频率的增加而增加。在构成TMJ盘的组分中，蛋白聚糖在确定盘的粘弹性中起重要作用，因此，赋予盘更大的分散和减少应力的能力。关于颞下颌关节的润滑，我们进行了第一次调查，以澄清完整的颞下颌关节的润滑能力。在50 N恒载荷作用5 s后，猪完整TMJ的平均摩擦系数为0.0145。摩擦系数随加载时间的延长而增大，1h后为0.022，高分子量透明质酸的加入在一定程度上可以恢复类OA结构降低的润滑性能。考虑到这些发现，我们开始做组织工程，颞下颌关节盘。考虑到细胞外基质的组成和细胞类型，血小板源性生长因子（PDGF）可能对TMJ椎间盘细胞的增殖和代谢有效。因此，我们研究了添加PDGF对TMJ椎间盘细胞增殖和细胞外基质合成的影响。结果表明，高剂量PDGF对体外培养的椎间盘细胞增殖、胶原和透明质酸合成的影响最大。这意味着PDGF在椎间盘退变的治疗中，特别是在组织工程化颞下颌关节盘的治疗中具有潜在的应用前景

项目成果

Eiji Tanaka: "Impulsive compression influences the viscous behavior of porcine temporomandibular joint disc."European Journal of Oral Sciences. 111(4). 353-358 (2003)

Eiji Tanaka：“脉冲压缩影响猪颞下颌关节盘的粘性行为。”欧洲口腔科学杂志。

Tanaka E, Aoyama J, Tanaka M, et al.: "The proteoglycan contents of the temporomandibular joint disc influence its dynamic viscoelastic properties"Journal of Biomedical Materials Research. (in press).

Tanaka E、Aoyama J、Tanaka M 等人：“颞下颌关节盘的蛋白多糖含量影响其动态粘弹性特性”生物医学材料研究杂志。

Tanaka E, Aoyama J, Tanaka M, et al.: "The proteoglycan contents of the temporomandibular joint disc influence its dynamic viscoelastic properties"Journal of Biomedical Materials Research. 65A(3). 386-392 (2003)

Tanaka E、Aoyama J、Tanaka M 等人：“颞下颌关节盘的蛋白多糖含量影响其动态粘弹性特性”生物医学材料研究杂志。

Tanaka E, del Pozo R, Tanaka M, et al.: "Strain rate effect on the biomechanical response of bovine temporomandibular joint disk under compression"Journal of Biomedical Materials Research. (in press).

Tanaka E、del Pozo R、Tanaka M 等人：“应变率对压缩下牛颞下颌关节盘生物力学反应的影响”生物医学材料研究杂志。

Eiji Tanaka: "Strain-rate effect on the biomechanical response of bovine temporomandibular joint disk under compression."Journal of Biomedical Materials Research. 67A(3). 761-765 (2003)

Eiji Tanaka：“应变率对压缩下牛颞下颌关节盘生物力学反应的影响。”生物医学材料研究杂志。