Glutamate receptor antagonist reduces pain following experimental tooth movement

谷氨酸受体拮抗剂可减轻实验性牙齿移动后的疼痛

• 批准号: 16592045
• 负责人: TANAKA Eiji
• 金额: $ 2.24万
• 依托单位: HIROSIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16592045/
• 关键词: Central nerve system; Glutamate; NMDA-receptor; Pain transmission; MK-801; Serotonin; Descending modulation; 実験的歯の移動

Various studies reported c-Fos expression in the neurons in the trigeminal sensory nuclear complex (TSNC) following experimental tooth movement, which implies pain transmission to the central nervous system. Meanwhile, MK-801, a non-competitive antagonist of N-methyl-D-aspartate (NMDA) receptors, was shown to markedly reduce the expression of c-Fos in the trigeminal subnucleus caudalis (Vc) following noxious stimulation, but to enhance c-Fos expression markedly in other brain regions, i.e., the neocortex, dorsal raphe and thalamic nuclei. In the present study, we examined the nature of c-Fos expression in the brainstem including the TSNC and mid-brain following administration of MK-801 and/or experimental movement of the rat molars. Twelve hours after the beginning of experimental tooth movement, c-Fos was expressed bilaterally in the superficial laminae of Vc (Vc I/II), dorsomedial areas of the trigeminal subnucleus oralis (Vodm) and rostro-dorsomedial areas of the trigeminal subnucleus oralis (Vor) with the ipsilaterally dominant distribution, but hardly in the periaqueductal gray (PAG), dorsal raphe nucleus (DR) and Edinger-Westphal nucleus (EW). Intraperitoneal administration of MK-801 (0.03,0.3 and 3.0 mg/kg) prior to the onset of experimental tooth movement reduced c-Fos in the TSNC (Vc I/II, Vodm and Vor), but increased it in the nucleus raphe magnus (NRM), ventrolateral PAG (vl PAG), DR and EW. These results highly emphasize that during experimental tooth movement, a blockade of NMDA receptors induces neuronal suppression in the TSNC, but increases neuronal activity in the descending antinociceptive system including the NRM, vl PAC, DR and EW.

各种研究报道了实验性牙齿移动后三叉神经感觉核复合体（TSNC）中的神经元中的c-Fos表达，这意味着疼痛传递到中枢神经系统。同时，N-甲基-D-天冬氨酸（NMDA）受体的非竞争性拮抗剂MK-801可显著降低伤害性刺激后三叉神经尾侧亚核（Vc）的c-Fos表达，但可显著增强其他脑区的c-Fos表达，新皮层、中缝背核和丘脑核。在本研究中，我们研究了MK-801给药和/或大鼠磨牙实验运动后脑干（包括TSNC和中脑）中c-Fos表达的性质。实验性牙齿移动后12 h，c-Fos在双侧Vc浅层（Vc I/II）、三叉神经口侧亚核背内侧区（Vodm）和三叉神经口侧亚核吻背内侧区（Vor）表达，呈同侧优势分布，而在中脑导水管周围灰质（PAG）、中缝背核（DR）和Edinger-Westphal核（EW）几乎不表达。在实验性牙齿移动开始前腹腔注射MK-801（0.03，0.3和3.0 mg/kg）可降低TSNC（Vc I/II，Vodm和Vor）中的c-Fos，但增加中缝大核（NRM）、腹外侧PAG（vl PAG）、DR和EW中的c-Fos。这些结果高度强调，在实验性牙齿移动期间，NMDA受体的阻断诱导TSNC中的神经元抑制，但增加包括NRM、vl PAC、DR和EW的下行抗伤害感受系统中的神经元活性。

项目成果

Administration of MK-801 decreases c-Fos expression in the trigeminal sensory nuclear complex but increases it in the midbrain.

施用 MK-801 会降低三叉神经感觉核复合体中的 c-Fos 表达，但会增加中脑中的 c-Fos 表达。

• 发表时间: 2004
• 期刊: Brain Research 1021・2
• 作者: Hattori Y;Watanabe M;Iwabe T;Tanaka E;et al.
• 通讯作者: et al.

Solid diet mastication reduces c-Fos expression in the rat trigeminal subnucleus caudalis following orofacial inflammation induced by CFA

固体饮食咀嚼可降低 CFA 诱导的口面部炎症后大鼠三叉神经尾亚核中 c-Fos 的表达

• 发表时间: 2006
• 期刊: Biomedical Research India (IN PRESS)
• 作者: Mika Nishi;Tatsunori Iwabe;Eiji Tanaka;Yukiko Hattori-Myoishi;Nobuhiko Kawai;Youhei Suekawa;Koji Takahashi;Mineo Watanabe;Takashi Uchida;Kazuo Tanne
• 通讯作者: Kazuo Tanne

Solid diet mastication reduced c-Fos expression in the rat trigeminal subnucleus caudalis following orofacial inflammation induced by CFA.

在 CFA 诱导的口面部炎症后，固体饮食咀嚼降低了大鼠三叉神经尾亚核中 c-Fos 的表达。

• 发表时间: 2006
• 期刊: Biomedical Research India 17-1
• 作者: Nishi M;Iwabe T;Tanaka E;et al.;Mika Nishi
• 通讯作者: Mika Nishi

Fiber type composition of rabbit jaw muscles is related to their daily activity.

兔子颌骨肌肉的纤维类型组成与其日常活动有关。

• 发表时间: 2005
• 期刊: European Journal of Neuroscience 22・11
• 作者: van Wessel T;Langenbach GEJ;Tanaka E;et al.
• 通讯作者: et al.