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Identification of host genetic factor influencing dental caries susceptibility in inbred mice

影响近交系小鼠龋齿易感性的宿主遗传因素的鉴定

基本信息

批准号：
14571972
负责人：
ASADA Yoshinobu
金额：
$ 2.11万
依托单位：
Tsurumi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571972/
关键词：
Inbred mice dental caries host genetic factor QTL caries susceptibility LRS score 齲蝕近交系マウス量的遺伝 DNAマーカー齲蝕症

项目摘要

The purpose of this study was to identify the host genetic factor influencing susceptibility to dental caries in inbred mice. Genetic factor(s) affecting dental caries in mice were examined using two inbred strains of mice, BALB/cJ and C3H/HeJ, and these genetic crosses in which were orally inoculated with Streptococcus mutans serotype c (1×10^8 CFU/mouse). In this study, Quantitative Trait Locus(QTL) analysis was used to identify the candidate chromosomes for gene(s) influencing dental caries susceptibility in mice. Two significant QTLs on chromosomes 7 and 11, and five suggestive QTLs on chromosomes 3,8,16 and 17 were detected. Around the 50 cM on chromosome 7 and 46 cM on chromosome 11,the likelihood ratio statistic(LRS) scores showed higher than significant levels. These results suggest that the major gene(s) responsible for caries susceptibility is located in these regions on chromosomes 7 and 11.We performed fine mapping of affected gene(s) to dental caries susceptibility on mouse chromosomes 7 and 11. It was showed a higher than suggestive level with the LRS scores between the markers D7Mit62 and D7Mit126,corresponded to 42.6 cM and 50 cM from centromere. It was also showed a higher than significant level with the LRS scores between the markers D11Mit242 and D11Mit262,corresponded to 31 cM and 46 cM from centromere. These results suggest that the major gene(s) responsible for caries susceptibility is located in these regions on chromosomes 7 and 11.

本研究的目的是确定影响近交系小鼠龋齿易感性的宿主遗传因素。采用两种近交系小鼠BALB/cJ和C3H/HeJ检测影响小鼠龋齿的遗传因素，并在这些遗传杂交中口服c型变形链球菌（1×10^8 CFU/小鼠）。本研究采用定量性状位点（QTL）分析方法，鉴定影响小鼠龋易感性的候选基因染色体。在7号和11号染色体上检测到2个显著qtl，在3号、8号、16号和17号染色体上检测到5个提示qtl。在7号染色体50 cM和11号染色体46 cM附近，似然比统计（LRS）得分均高于显著水平。这些结果表明，负责龋易感性的主要基因位于7号和11号染色体上的这些区域。我们对小鼠7号和11号染色体上的龋易感性基因进行了精细定位。标记D7Mit62和D7Mit126的LRS评分高于提示水平，分别距离着丝粒42.6 cM和50 cM。标记D11Mit242和D11Mit262之间的LRS评分也高于显著水平，分别对应着丝粒31 cM和46 cM。这些结果表明，负责龋易感性的主要基因位于7号和11号染色体上的这些区域。