Molecular genetic study of the specific tooth agenesis in the third molar of EL mice

EL小鼠第三磨牙特定牙齿发育不全的分子遗传学研究

• 批准号: 23593053
• 负责人: ASADA Yoshinobu
• 金额: $ 3.33万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23593053/
• 关键词: 歯胚消失; ELマウス; 第三臼歯; マイクロアレイ解析; 遺伝発現解析; Lef１遺伝子; Shh遺伝子; LAMA5遺伝子; インテグリン受容体

A detailed study using Real time PCR, in situ hybridization, immunohistological analysis and DNA microarray revealed that the absence of third molars in EL/sea mice is strongly controlled by the interaction between beta 1 integrin and laminin alpha 5. These results suggest that Apoptosis and Hedgehog pathways are associated with the development of the tooth germs of M3 in the bud stage of EL mice.We concluded that the specific tooth agenesis in EL/sea mice is regulated by the basement membrane proteins such as beta 1 integrin and laminin alpha 5.

使用真实的时间PCR、原位杂交、免疫组织学分析和DNA微阵列的详细研究揭示，EL/sea小鼠中第三磨牙的缺失强烈地受β 1整合素和层粘连蛋白α 5之间的相互作用控制。这些结果表明，凋亡和Hedgehog通路与EL/sea小鼠牙胚发育有关，EL/sea小鼠牙胚发育受基底膜蛋白β 1整合素和层粘连蛋白α 5的调控。

项目成果

歯種特異的な歯胚消失メカニズムの解明に向けて

阐明特定牙齿类型的牙胚损失机制

• 发表时间: 2013
• 作者: 成山明具美;伊平弥生;宗正隆明;朝田芳信
• 通讯作者: 朝田芳信

Molecular genetic study of specific missing in the third molar of EL mice

EL小鼠第三磨牙特异性缺失的分子遗传学研究

• 作者: 成山明具美;伊平弥生;宗正隆明;朝田芳信
• 通讯作者: 朝田芳信