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Study of the CSF-1 gene effects causing absence of the third molars in mice

CSF-1基因影响导致小鼠第三磨牙缺失的研究

基本信息

批准号：
12672020
负责人：
ASADA Yoshinobu
金额：
$ 0.51万
依托单位：
Tsurumi University (2001)Nihon University (2000)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672020/
关键词：
EL mice missing teeth molecular genetic linkage analysis tooth anomaly 欠如歯 SSLPマーカー遺伝的交配実験

项目摘要

To identify the candidate chromosome and to detect the region that included causative gene(s) causing absence of the third molars in EL mice, all chromosomes were examined by the individual genotyping of the F2 (EL x MSM) mice. The gene causing absence of the third molars in EL mice was mapped on the region from D3Mit141 to D3Mit290 (x 2 =22.8, p<0.0001). Gene such as Lef1, Egf and CSF-1 that have been shown to be associated with tooth development, are located in this region. To determine whether the congenital absence of the third molars in EL mice occurs due to failure of initiation or subsequent arrest in development, I analyzed H ＆ E stained serial sagittal sections of normal and EL mice. Compared to normal mice, third molars development in EL mice progressed from early cap to cap stage from P3 to P5, but did not progress beyond the cap stage and the developing tooth gradually regressed to complete elimination. I examined whether the absence of the third molars in EL mice occurred due to lack of Lef1 expression. Lef1 expression in EL mice was not different from that observed in normal mice. From these data, it was concluded that Lef1 is not a likely candidate gene involved in the congenital absence of the third molars in EL mice. On the other hand, the candidate chromosomal analysis was also performed on genetic crosses using two strains of mice, EL and DDY. The highest scores were found at D13Mit78 and D13Mit35 markers (x2 =11.8, p<0.01). Based on these results, there was a possibility that a candidate gene causing absence of the third molars might be existed on mouse chromosome 3.

为了鉴定候选染色体并检测包括导致EL小鼠中第三磨牙缺失的致病基因的区域，通过F2（EL x MSM）小鼠的个体基因分型来检查所有染色体。导致EL小鼠第三磨牙缺失的基因定位于D3 Mit 141至D3 Mit 290区域（x 2 =22.8，p<0.0001）。与牙齿发育相关的基因如Lef 1、Egf和CSF-1都位于该区域。为了确定EL小鼠第三磨牙的先天性缺失是否是由于启动失败或随后的发育停滞而发生的，我分析了正常和EL小鼠的H & E染色的连续矢状切片。与正常小鼠相比，EL小鼠第三磨牙的发育从早期帽状期进展到帽状期，从P3到P5，但没有进展到帽状期之后，发育中的牙齿逐渐退化到完全消失。我检查了EL小鼠第三磨牙的缺失是否是由于Lef 1表达的缺乏而发生的。EL小鼠中Lef 1的表达与正常小鼠中观察到的没有差异。从这些数据中，可以得出结论，Lef 1是不是一个可能的候选基因参与先天性缺失的第三磨牙在EL小鼠。另一方面，还使用两个品系的小鼠EL和DDY对遗传杂交进行了候选染色体分析。在D13 Mit 78和D13 Mit 35标记物处发现最高得分（x2 =11.8，p<0.01）。根据这些结果，有可能是一个候选基因造成的缺失第三磨牙可能存在于小鼠3号染色体上。