Research on macrophage secretion regulation ingredients in Bidens genus plants

本文研究了小花鬼针草（Bidens parviflora）对巨噬细胞分泌NO的抑制作用和对前列腺素E_2（PGE_2）的抑制作用。鬼针草（Bidens parviflora）是我国民间常用的解热、抗风湿药。从该植物中分离得到61个化合物，用脂多糖（LPS）和γ-干扰素（IFN-γ）激活小鼠巨噬细胞样细胞株RAW264.7，体外抗过敏反应，抑制一氧化氮（NO）的产生，并抑制大鼠腹腔肥大细胞释放组胺。分离得到5个新的聚乙炔糖苷，25个含2个新化合物的黄酮类化合物，1个新的二聚咖啡酸衍生物，2个新的咖啡酸酯，2个咖啡酸衍生物，2个肉桂酸衍生物，2个新木脂素，1个含10个碳链的环戊烯糖苷，8个奎尼酸酯，1个苄醇糖苷，1个萘并吡喃酮糖苷，1个类胡萝卜素，1个二萜，在五种聚乙酰烯葡糖苷中，四种对NO产生显示出强抑制活性，并且关于这些汞合金的巨噬细胞的给予NO产物性能的效果，由于已经检查了在用IFN-γ和LPS恢复活力时检查药物的作用，确认了抑制。在黄酮类化合物中，3 '，4' -位上有两个羟基的黄酮、黄酮醇和橙酮具有抑制NO生成的活性。此外，通过咖啡酸二聚体，甲基奎尼酸的咖啡酸酯，NO产物抑制活性被识别。本文研究了分离的化合物对LPS激活的巨噬细胞产生前列腺素E_2的抑制作用。蔗糖对羟基肉桂酸酯和咖啡酸衍生物对前列腺素E_2的合成有抑制作用。

We examined Bidens parviflora with NO production inhibitory effects and prostaglandin E_2 (PGE_2) inhibitory effect toward the macrophage secretions which had a relation in antiallergy and chronic inflammation. Chinese folk medicine, Bidens parviflora (Compositae) has been used as a traditional antipyretic and anti-rheumatic medicine. Sixty-one compounds isolated from this whole plant were examined for anti-allergic actively toward nitric oxide (NO) production by a murine macrophage-like cell line, RAW264.7, activated with lipopolysaccharide (LPS) and interferon-γ(IFN-γ) and histamine release from rat peritoneal mast cells in vitro. We isolated five new polyacetylene glucosides, twenty-five flavonoides containing two new compounds, a new dimeric caffeic acid derivative, two new caffeic acid esters, two caffeic acid derivatives, two cinnamic acid derivatives, two new neolignans, a cyclopentene glycoside having ten carbon chain, eight quinic acid esters, a benzyl alcohol glycoside, a naphthopyrone glycoside, carotinoids, a diterpene, sterols and myo-inositol.Among five polyacetilene glucosides, four exhibited strong inhibitory activities toward NO production and, about effect to give to NO product performance of a macrophage of these amalgam, restraint was recognized as a result of having examined action of an examination drug at having been revitalized with IFN-γ and LPS. In the flavonoids, NO product inhibitory activities were recognized by flavones, flavonols and aurones which had two hydroxyl groups to 3', 4' -position. In addition, NO product inhibitory activities were recognized, by a caffeic acid dimer, a caffeic acid ester of methyl quinic acid. We investigated the inhibitory effects of isolated compounds on prostaglandin (PG) E_2 production in LPS-activated macrophages. About sucrose p-hydroxylcinnamic acid esters and caffeic acid derivatives, PGE_2 production inhibitory activities were recognized by several compounds.