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Research on macrophage secretion regulation ingredients in Bidens genus plants

鬼针草属植物巨噬细胞分泌调节成分的研究

基本信息

批准号：
12672070
负责人：
KITANAKA Susumu
金额：
$ 2.18万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

We examined Bidens parviflora with NO production inhibitory effects and prostaglandin E_2 (PGE_2) inhibitory effect toward the macrophage secretions which had a relation in antiallergy and chronic inflammation. Chinese folk medicine, Bidens parviflora (Compositae) has been used as a traditional antipyretic and anti-rheumatic medicine. Sixty-one compounds isolated from this whole plant were examined for anti-allergic actively toward nitric oxide (NO) production by a murine macrophage-like cell line, RAW264.7, activated with lipopolysaccharide (LPS) and interferon-γ(IFN-γ) and histamine release from rat peritoneal mast cells in vitro. We isolated five new polyacetylene glucosides, twenty-five flavonoides containing two new compounds, a new dimeric caffeic acid derivative, two new caffeic acid esters, two caffeic acid derivatives, two cinnamic acid derivatives, two new neolignans, a cyclopentene glycoside having ten carbon chain, eight quinic acid esters, a benzyl alcohol glycoside, a naphthopyrone glycoside, carotinoids, a diterpene, sterols and myo-inositol.Among five polyacetilene glucosides, four exhibited strong inhibitory activities toward NO production and, about effect to give to NO product performance of a macrophage of these amalgam, restraint was recognized as a result of having examined action of an examination drug at having been revitalized with IFN-γ and LPS. In the flavonoids, NO product inhibitory activities were recognized by flavones, flavonols and aurones which had two hydroxyl groups to 3', 4' -position. In addition, NO product inhibitory activities were recognized, by a caffeic acid dimer, a caffeic acid ester of methyl quinic acid. We investigated the inhibitory effects of isolated compounds on prostaglandin (PG) E_2 production in LPS-activated macrophages. About sucrose p-hydroxylcinnamic acid esters and caffeic acid derivatives, PGE_2 production inhibitory activities were recognized by several compounds.

研究了小花拜登对巨噬细胞分泌一氧化氮的抑制作用和前列腺素e2 （PGE_2）的抑制作用，并探讨了其抗过敏和慢性炎症的作用。中国民间药物，拜登草（菊科）已被用作传统的解热和抗风湿药。采用小鼠巨噬细胞样细胞系RAW264.7，通过脂多糖（LPS）、干扰素-γ(IFN-γ)和组胺的体外释放，研究了从该植物中分离的61种化合物对一氧化氮（NO）产生的抗过敏活性。我们分离到了5种新的聚乙炔糖苷、25种含2种新化合物的黄酮类化合物、1种新的二聚咖啡酸衍生物、2种新的咖啡酸酯、2种咖啡酸衍生物、2种肉桂酸衍生物、2种新的新木脂素、1种含10个碳链的环戊烯糖苷、8种醌酸酯、1种苯甲醇糖苷、1种萘吡啶糖苷、类胡萝卜素、1种二萜、1种甾醇和肌醇。在五种聚乙炔糖苷中，有四种对NO的产生表现出强烈的抑制活性，关于这些汞齐对巨噬细胞NO产物性能的影响，抑制作用被认为是检测药物在IFN-γ和LPS活化后的作用的结果。在黄酮类化合物中，3′、4′位置有两个羟基的黄酮类化合物、黄酮醇类化合物和金酮类化合物具有抑制NO产物的活性。此外，通过咖啡酸二聚体（咖啡酸甲酰奎宁酸酯）可以识别NO产物的抑制活性。我们研究了分离化合物对lps激活巨噬细胞中前列腺素E_2生成的抑制作用。对于蔗糖对羟基肉桂酸酯和咖啡酸衍生物，有几种化合物具有抑制PGE_2生成的活性。