Functions and behavior of oxidized protein hydrolase in cells

细胞中氧化蛋白水解酶的功能和行为

• 批准号: 14572070
• 负责人: KIKUGAWA Kiyomi
• 金额: $ 2.24万
• 依托单位: Tokyo University of Pharmacy and Life Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14572070/
• 关键词: oxidized protein; oxidized protein hydrolase (OPH); COS-7 cell; oxidative stress; proteasome; OPH

Functions and behavior of newly found oxidized protein hydrolase (OPH) in cells were investigated. It was found that the enzyme acted as a secondary antioxidant system in cells by prevention of the accumulation of oxidized proteins in cells.COS-7 cells overexpressing OPH (COS-7-OPH) were established. While protein carbonyls were accumulated in pareteral COS-7 cells depending on the degree of oxidative stress induced by hydrogen peroxide and paraquat, protein carbonyls were not accumulated in COS-7-OPH cells. While COS-7 cells were dead depending on the degree of oxidative stress, the cell death of COS-7-OPH cells induced by the oxidative stress was inhibited. When COS-7-OPH cells were treated with an inhibitor of OPH, paraquat-induced accumulation of protein carbonyls and the degree of cell death were increased. In contrast, when COS-7-OPH cells were treated with an inhibitor of proteasome, paraquat-induced accumulation of protein carbonyls and the degree of cell death were not increased. These findings demonstrated that OPH protected cell death of COS-7-OPH cells from oxidative stress.

研究了细胞中新发现的氧化蛋白水解酶（OPH）的功能和行为。发现该酶通过预防细胞中氧化蛋白的积累来充当细胞中的二级抗氧化剂系统。COS-7细胞过表达OPH（COS-7-OPH）。尽管蛋白羰基积聚在对pareteral cos-7细胞中，具体取决于过氧化氢诱导的氧化应激程度和paraquat，但蛋白羰基在cos-7-oph细胞中均未积聚。虽然COS-7细胞根据氧化应激程度而死亡，但抑制了由氧化应激诱导的COS-7-oph细胞的细胞死亡。当用OPH的抑制剂处理COS-7-OPH细胞时，会诱导的蛋白羰基积累并增加了细胞死亡程度。相反，当用蛋白酶体的抑制剂处理cos-7-oph细胞时，paraquat诱导的蛋白羰基积聚和细胞死亡程度不会增加。这些发现表明，OPH保护了COS-7-OPH细胞的细胞死亡免受氧化应激。

项目成果

K.Shimizu, T.Fujino, K.Ando, M.Hayakawa, Y.Yasuda, K.Kikugawa: "Overexpression of oxidized protein hydrolase (OPH) protects COS-7 cells from oxidative stress-induced inhibition of cell growth and survival"Biochem.Biophys.Res.Commun.. 304. 766-771 (2003)

K.Shimizu、T.Fujino、K.Ando、M.Hayakawa、Y.Yasuda、K.Kikukawa：“氧化蛋白水解酶 (OPH) 的过度表达可保护 COS-7 细胞免受氧化应激诱导的细胞生长和存活抑制”

K.Shimizu, T.Fujino, K.Kikugawa: "Overexpression of oxidized protein hydrolase protects COS-7 cells from oxidative stress-induced inhibition of cell growth and survival"Biochem.Biophys.Res.Commun.. 304. 766-771 (2003)

K.Shimizu、T.Fujino、K.Kikukawa：“氧化蛋白水解酶的过度表达可保护 COS-7 细胞免受氧化应激诱导的细胞生长和存活抑制”Biochem.Biophys.Res.Commun.. 304. 766-771（

K.Kikugawa: "Damage caused by oxidative stress in living bodies and its defense"Oleo Science.. 2. 723-738 (2002)

K.Kikukawa：“生物体内氧化应激造成的损害及其防御”Oleo Science.. 2. 723-738 (2002)

菊川清見: "酸化的ストレスの生体内反応とその防除に関する研究"オレオサイエンス. 2. 733-738 (2002)

Kiyomi Kikukawa：“氧化应激体内反应及其控制的研究”Oleoscience 2. 733-738 (2002)。

K Shimizu, T.Fujino, K.Ando, K.Kikugawa: "Overexpression of oxidized protein hydrolase protects COS-7 cells from oxidative stress-induced inhibition"Biochem.Biophys.Res.Commun.. 304. 766-771 (2003)

K Shimizu、T.Fujino、K.Ando、K.Kikukawa：“氧化蛋白水解酶的过度表达保护 COS-7 细胞免受氧化应激诱导的抑制”Biochem.Biophys.Res.Commun.. 304. 766-771 (2003)