Involvement of Lipid Oxidation Products in Erythrocyte Membrane Damage

脂质氧化产物参与红细胞膜损伤

基本信息

批准号：
60571060
负责人：
KIKUGAWA Kiyomi
金额：
$ 0.77万
依托单位：
Tokyo College of Pharmacy
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60571060/
关键词：
Erythrocyte Cell membrane Lipid oxidation Membrane damage Membrane protein Malonaldehyde Aldehyde ケイ光

项目摘要

1. Studies on the molecules of lipid oxidation products involved in erythrocyte membrane damage.Covalent modification of the membrane components, such as formation of fluorescent proteins and protein cross-linking, by oxidized lipids is a damaging reaction to the membrane and the cells. Malonaldehyde has been believed to be the major causative molecule of oxidized lipids involved in the protein modifications. However, our studies in various amino compounds and erythrocyte membranes have suggested that monofunctional aldehydes but not malonaldehyde are the major molecules involved in the modifications.2. Factors affecting lipid oxidation of erythrocyte membranes.We have shown that hemoglobin plays a catalytic role in erythrocyte membrane lipid oxidation, and its effect decreases at high concentrations.It was also found that periodate, an oxidizing agent known to induce various biological responces of viable cells, induces lipid oxidation of erythrocyte membranes and the oxidation depends on hemoglobin and the sialyl residues of the cell surface.3. Detection of erythrocyte membrane proteins modified with oxidized lipids.A method for the selective radiolabeling of the membrane proteins modified with oxidized lipids has been developed. The radiolabeling was achieved by use of tritiated borohydride, and this method permitted detection and identification of the erythrocyte membrane proteins modified with oxidized lipids.

1。对涉及红细胞膜损伤的脂质氧化产物的分子的研究。膜成分的可价修饰，例如形成荧光蛋白的形成和蛋白质交联，氧化脂质是对膜和细胞的损害反应。据信丙醛是参与蛋白质修饰的氧化脂质的主要致病分子。但是，我们在各种氨基化合物和红细胞膜上的研究表明，单功能醛（但不是马洛醛）是参与修饰的主要分子。2。影响红细胞膜的脂质氧化的因素。我们表明，血红蛋白在红细胞膜脂质氧化中起催化作用，其效应在高浓度下降低，这也是建立的，这也是氧化剂，一种已知的氧化剂，可诱导氧化细胞的各种生物学氧化物，并引起氧化氧化的氧化，并引起氧化氧化的氧化氧化物，氧化氧化物的氧化氧化物，氧化氧化物的氧化液体，氧化氧化。取决于血红蛋白和细胞表面的siAllyl残基3。已经开发了用于选择性化脂质蛋白质的方法的红细胞膜蛋白的检测。通过使用Tritied Borohydride来实现放射性标记，该方法允许检测和鉴定用氧化脂质修饰的红细胞膜蛋白。