Protection against oxidative stress by polyunsaturated fatty acids

多不饱和脂肪酸防止氧化应激

基本信息

批准号：
09672257
负责人：
KIKUGAWA Kiyomi
金额：
$ 2.05万
依托单位：
Tokyo University of Pharmacy and Life Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672257/
关键词：
Reactive oxyten species Oxidative stress Polyunsaturated fatty acid Lipid peroxidation Aging Thiobarbituric acid test 脂質過酸化赤血球

项目摘要

1. Use and limitation of thiobarbituric acid (TBA) test for lipid peroxidation was clarified.2. Wistar male rats were fed a diet containing n-6 fatty acid-rich safflower oil or n-3 fatty acid-rich fish oil with an equal amunt of vitamin E for 6 weeks. n-3 Fatty acid content in erythrocyte membranes of rats fed fish oil was significantly higher than that of rats fed safflower oil. The degree of membrane lipid peroxidation and protein aggregation of ras fed fish oil was not significantly higher than that of rats fed safflower oil when the amounts of phospholipid hydroperoxides, TBA-reactive substances and detergent-insoluble protein aggregaates were measured. If a sufficient amount of vitamin E is supplied, n-3 fatty acid supplementation may give no undeseirable oxidative effects on rat erythrocytes in the circulation.3. Wistar male rats were fed a diet containing safflower oil orfish oil with an equal amunt of vitamin E for 6 weeks. n-3 Fatty acid contents in lipids in brain, liver, lung, heazt, kidney, spleen, stomach ant testis were significantly higher than those of rats fed safflower oil. The degree of lipid peroxidation of the organs of ras fed fish oil was not significantly higher than that of rats fed safflower oil when the amounts of phospholipid hydroperoxides and TBA-reactive substances were measured. n-3 Fatty acid supplementation may give no undeseirable oxidative effects on rat organs.

1。阐明了硫豆吡啶甲酸（TBA）检测脂质过氧化的测试。2。 Wistar雄性大鼠饮食中含有N-6脂肪酸富含红花的红花油或N-3脂肪酸富含维生素E的饮食，持续6周。喂养鱼油的大鼠红细胞膜中的N-3脂肪酸含量明显高于饲喂红花油的大鼠。当磷脂氢过氧化物的量，TBA反应物质和去污剂 - 不溶性蛋白质聚集酸盐的含量时，喂入红花油的膜脂质过氧化和蛋白质聚集的程度不高于饲喂红花油的大鼠的程度。如果提供了足够量的维生素E，则补充N-3脂肪酸可能不会对循环中大鼠红细胞的不可息氧化作用。3。 wistar雄性大鼠饮食中含有带状油或鱼的饮食，并用同等的维生素E饮食持续6周。 N-3脂肪酸含量的脂质含量在大脑，肝脏，肺，Heazt，肾脏，脾脏，胃蚂蚁睾丸明显高于饲喂红花油的大鼠。当测量磷脂氢过氧化物和TBA反应性物质的量时，喂养鱼油器官器官的脂质过氧化程度不高于饲喂红花油的大鼠。补充N-3脂肪酸可能不会对大鼠器官产生不可息的氧化作用。