Effects of heat shock protein and calcineurin in hypertrophied and atrophied skeletal muscles

热休克蛋白和钙调神经磷酸酶对肥大和萎缩骨骼肌的影响

• 批准号: 16500414
• 负责人: OISHI Yasuharu
• 金额: $ 2.37万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500414/
• 关键词: Heat shock protein; calcineurin; muscle hypertrophy; muscle atrophy; myosin heavy chain; Hsp72; rat; fiber type; Heat shock protein; 筋肥大; 筋萎縮; HSP72

During passed two years, I investigated the effects of heat shock proteins (HSP) and calcineurin (CaN) in hypertrophied or atrophied rat skeletal muscles. In clenbuterol injected rat hindlimb muscles, which occurred hypertrophy and the shift of fiber phenotype from slow-to-fast, Hsp72 was decreased in the slow soleus muscle, where the CaN was increased. Functionally overload (FO) of fast plantaris muscle by cutting the tendons of its synergists induced muscle fiber hypertrophy and the shift of fiber phenotype from fast-to-slow. Hsp72 was also upregulated in functionally overloaded plantaris concomitantly with the increase in fiber size and slow fiber phenotype. Muscle hypertrophy and the shift of fiber phenotype observed in the FO plantaris was inhibited by the treatment of cyclosporin A, a inhibitor of CaN, indicating that the CaN was related to the fiber size and phenotype. In addition, thyroid hormone (T3) treatment to the FO plantaris downregulated to the expression level of the Hsp72 and CaN.

在过去的两年中，我研究了热休克蛋白（HSP）和钙调神经磷酸酶（CaN）在肥大和萎缩的大鼠骨骼肌中的作用。在注射克伦特罗的大鼠后肢肌肉中，发生肥大和纤维表型从慢到快的转变，在慢比目鱼肌中Hsp72减少，其中CaN增加。通过切断其协同者的肌腱使快速跖肌功能超负荷（FO）诱导肌纤维肥大和纤维表型由快向慢转变。热休克蛋白72也上调功能超载足底伴随着纤维大小和慢纤维表型的增加。CaN抑制剂环孢菌素A（cyclosporin A）可抑制足跖肌的肥大和肌纤维表型的改变，提示CaN与肌纤维的大小和表型有关。此外，甲状腺激素（T3）处理的FO跖下调Hsp72和CaN的表达水平。

项目成果

不活動と筋萎縮のメカニズム

不活动和肌肉萎缩的机制

• 发表时间: 2005
• 期刊: 体育の科学 55
• 作者: M.Tanaka;Y.Oshida et al.;大石康晴
• 通讯作者: 大石康晴

Adaptation of skeletal muscle cells to contractile stress.

骨骼肌细胞对收缩应激的适应。

• 发表时间: 2005
• 期刊: The Science of Physical Education 55
• 作者: Yasuharu Oishi;Tomonori Ogata
• 通讯作者: Tomonori Ogata

Calcineurin and heat shock proteins modulation in clembuterol induced hypertrophied rat skeletal muscle

钙调神经磷酸酶和热休克蛋白对克伦特罗诱导的大鼠骨骼肌肥大的调节

• 发表时间: 2004
• 期刊: Pflugers Archive European Journal of Physiology 448
• 作者: Oishi Y;et al.
• 通讯作者: et al.

Effects of T3 treatment on HSP72 and calcineurin content of functionally overloaded rat plantaris muscle.

T3 治疗对功能超负荷大鼠跖肌 HSP72 和钙调神经磷酸酶含量的影响。

• 发表时间: 2005
• 期刊: Biochemical and Biophysical Research Communications 331
• 作者: Tomonori Ogata;Yasuharu Oishi;Roland R.Roy;Hajime Ohmori.
• 通讯作者: Hajime Ohmori.

Calcineurin and heat shock protein 72 in functionally overloaded rat plantaris muscle.

功能超负荷的大鼠跖肌中的钙调神经磷酸酶和热休克蛋白 72。

• 发表时间: 2005
• 期刊: Biochem. Biophys. Res. Commun. 330
• 作者: Oishi;Y.;T.Ogata;Y.Ohira;K.Taniguchi;R.R.Roy
• 通讯作者: R.R.Roy