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CHANGES OF THE EXPRESSION FOR THE STRESS PROTEINS IN RAT SKELETAL MUSCLES

大鼠骨骼肌应激蛋白表达的变化

基本信息

批准号：
12680041
负责人：
OISHI Yasuharu
金额：
$ 1.86万
依托单位：
KUMAMOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680041/
关键词：
heat shock proteins myosin heavy chain HSP72 muscle hypertrophy muscle atrophy fiber type composition muscle injury soleus heat shock protein ミオシン重鎖筋線維横断面積筋繊維組成ストレス蛋白質ブピバカイン筋損傷ヒラメ筋

项目摘要

We performed two experiments in 2000-2002. One is the expression of heat shock proteins (HSPs) in regenerating rat soleus muscle, and the other is the expression of HSPs in hypertrophied rat soleus muscle.(1) The expression of HSPs in regenerating rat soleus muscle : the soleus muscle fibers were degenerated by injection of bupivacain. The regenerate fibers were observed at day 4 after the injection, and the fiber area gradually increased during recovery week 4. The HSP72 levels in regenerating fibers increased consistently with the growth for the regenerating fibers. These results may suggest that the HSP72 is related to the growth for the regenerating fibers.(2) The expression of HSPs in hypertrophied rat soleus muscle : soleus muscle fibers, especially the fast-twitch fibers, were hypertrophied by clenbuterol administration in drinking water for 4 weeks. The fiber type composition and the profiles of myosin heavy chain composition were changed toward "Fast" types in the slow soleus muscle treated with clenbuterol. Coexpression of myosin heavy chain isoforms were observed in the clenbuterol-treated soleus single fibers. However, the expression level of HSP72 was dramatically decreased in hypertrophied solesu muscle. Our results clearly indicate that the HSP72 was decreased in hypertrophied soleus muscle induced by clenbuterol, and suggest that the HSP72 is not related directly to the muscle hypertrophy.

我们在2000-2002年进行了两次实验。一个是热休克蛋白（HSPs）在再生大鼠比目鱼肌中的表达，另一个是热休克蛋白在肥厚大鼠比目鱼肌中的表达。(1)热休克蛋白在再生大鼠比目鱼肌中的表达：注射布比卡因使比目鱼肌纤维变性。注射后第4天观察再生纤维，恢复第4周纤维面积逐渐增大。再生纤维中HSP72水平随再生纤维的生长而升高。这些结果可能表明HSP72与再生纤维的生长有关。(2)热休克蛋白在肥厚的大鼠比目鱼肌中的表达：饮水给药4周后，比目鱼肌纤维，尤其是快抽动纤维出现肥厚。盐酸克仑特罗处理后，慢比目鱼肌纤维类型组成和肌球蛋白重链组成谱向“快”型转变。在克仑特罗处理的比目鱼单纤维中观察到肌球蛋白重链异构体的共表达。而在肥大的比目鱼肌中，HSP72的表达水平明显降低。我们的结果清楚地表明，HSP72在瘦肉精诱导的肥厚比目鱼肌中降低，提示HSP72与肌肉肥厚无直接关系。