Effects of apotesis-related proteins in the size and phenotypes of rat skeletal msucles

凋亡相关蛋白对大鼠骨骼肌大小和表型的影响

• 批准号: 18500511
• 负责人: OISHI Yasuharu
• 金额: $ 2.63万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500511/
• 关键词: Apoptosis; Soleus; Mvonuclear; Heat stress; Satellite cell; Hincllimb unloading; Hsp72; Calcineurin; 筋線維横断面積; ミオシン重鎖; 筋萎縮; 筋線維タイプ; ヒラメ筋; 筋肥大; 骨格筋; ラット

1. To investigate the apoptotic responses, rat soleus muscles were subjected to 14-day of hindlimb unloading (14U)and 14-day reloading. After HU, soleus fiber size and the myonuclear number/fiber were significantly decreased, while the apoptotic myonuclear number increased. During ambulatory recovery day〜14 after HU, fiber size and myonuclear number were gradually increased, concomitant with the decrease in apoptotic nuclei Our results suggested that soleus fiber atrophy was induced by the decrement of myonuclear number via, in part, by apoptotic response.2.We further analyzed the effects of heat stress (HS)on regenerating soleus fibers. The soleus fibers were degenerated by bupivacaine injection. Compared with regenerating fibers without HS, regenerating fibers with HS were greater in cross-sectional area and myonuclear numbers. Also, activation of satellite cells and the expression levels of heat shock protein (Hsp)72 and calcineurin were enhanced in HS-regenerating soleus fibers. Our results suggest that the growth of regenerating fibers is possible to be accelerated by heat stress via enhancement of satellite cell activation.

1.为了研究细胞凋亡的反应，大鼠比目鱼肌进行14天的后肢卸载（14 U）和14天的重新加载。HU后比目鱼肌纤维直径和肌纤维数明显减少，而凋亡肌纤维数增加。在HU后第14天恢复期，肌纤维体积和肌细胞数目逐渐增大，凋亡细胞核减少。本研究结果提示，肌细胞数目减少可能是导致比目鱼肌纤维萎缩的原因之一。注射布比卡因可使比目鱼肌纤维变性。与未加HS的再生纤维相比，加HS的再生纤维的横截面积和肌纤维数目均较大。此外，卫星细胞的激活和热休克蛋白（Hsp）72和钙调神经磷酸酶的表达水平在HS再生比目鱼肌纤维增强。我们的研究结果表明，再生纤维的生长可能是通过增强卫星细胞活化的热应力加速。

项目成果

発育期のラット骨格筋に対する加重力の影響

发育过程中加载力对大鼠骨骼肌的影响

• 发表时间: 2007
• 作者: Yasuharu;Oishi;et. al.;大石 康晴
• 通讯作者: 大石 康晴

Effects of hyper-gravity on developing rat skeletal Muscle

超重力对大鼠骨骼肌发育的影响

• 发表时间: 2007
• 作者: Yasuharu;Oishi;et. al.
• 通讯作者: et. al.

Cellular adaptations in soleus muscle during recovery after hindlimb unloading

• DOI: 10.1111/j.1748-1716.2007.01747.x
• 发表时间: 2008-03-01
• 期刊: ACTA PHYSIOLOGICA
• 影响因子: 6.3
• 作者: Oishi, Y.;Ogata, T.;Roy, R. R.
• 通讯作者: Roy, R. R.