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RNA LEGO : Construction of functional RNA nanostructures

RNA LEGO：功能性 RNA 纳米结构的构建

基本信息

批准号：
16510082
负责人：
HARADA Kazuo
金额：
$ 2.5万
依托单位：
Tokyo Gakugei University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16510082/
关键词：
RNA folding loop-loop interaction RNA nanostructures RNA dendrimers thermal isomerization peptide-binding triple-helical scaffold loop-loop (kissing) interaction RNA nanostructure RNA dendrimer

项目摘要

We have previously shown that RNA substrates consisting of two stem-loops connected by a short linker can be used to construct specific circular or linear self-assemblies through loop-loop interactions. We also showed that by adjusting the strength of the loop-loop interaction, the assembly and disassembly of the RNA structures could be controlled in a magnesium-dependent manner (Horiya et al., Chem.＆ Biol., 2003).During this project term, we obtained the following results:1) We showed that RNA substrates consisting of three stem-loops could be used to construct dendrimeric assemblies (Fujiya and Harada, Nucleic Acids Symp.Ser., 2004).2) We demonstrated that the interconversion between circular and linear RNA assemblies could be regulated by an external substrate such as magnesium ion (Ohmori et al., Nucleic Acids Symp.Ser., 2005).3) We showed that circular RNA assemblies can be efficiently isomerized to a thermodynamically stable structure. Using this structural isomerization as a functional switch, RNA substrates that assemble into circular dimers through loop-loop interactions, and isomerizes to form peptide binding sites and catalytic domains were designed (Li et al., J.Am.Chem.Soc., 2006).4) We showed that thermal isomerization can be used as a switch to construct complex nanostructures. This was accomplished by designing RNA loop-loop complexes that upon thermal isomerization form 5'- and 3'-single stranded regions complementary to each other. Such substrates can be used to form linear and two-dimensional assemblies (Kunimoto et al., in preparation).

我们以前已经证明，由两个由短连接子连接的茎环组成的RNA底物可以通过环-环相互作用来构建特定的环形或线性自组装。我们还表明，通过调节环-环相互作用的强度，RNA结构的组装和拆解可以以依赖于镁的方式进行控制(Horiya等人，Chem.&Biol.，2003)。在这个项目期间，我们获得了以下结果：1)我们证明了由三个茎环组成的RNA底物可以用来构建树状大分子组装件(Fujiya和Harada，Nucic Symp.Ser.，2004)。2)我们证明了环状和线形RNA组装件之间的相互转化可以被外部底物如镁离子调节(Ohmori等人，Nucleic Symp.Ser，3)我们证明了环形RNA组件可以有效地异构化成热力学稳定的结构。利用这种结构异构化作为功能开关，设计了通过环-环相互作用组装成环状二聚体的RNA底物，并通过异构化形成肽结合部位和催化结构域(Li等人，J.Am.Chem.Soc.，2006)。4)我们证明了热异构化可以作为开关来构建复杂的纳米结构。这是通过设计RNA环-环复合体来实现的，这些环-环复合体在热异构化时形成相互补充的5‘和3’单链区域。这种底物可用于形成线性和二维组件(Kurimoto等人，正在制备中)。