Infection-induced mutation rates of plant resistance genes

感染诱导的植物抗性基因突变率

• 批准号: 470688116
• 负责人: Professor Dr. Korbinian Schneeberger
• 金额: --
• 依托单位: Institut für Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/470688116?language=en
• 关键词: Infection; induced; mutation; rates; plant

In plants, the genomic make-up of genes involved in resistance against biotic stress (so-called R-genes) involves a striking enrichment of forward (facing the same direction) tandem duplications. Moreover, for a long time R-gene clusters are known for their high diversity between individual accessions. Together this suggests that R-gene clusters evolve through rapid accumulation of forward tandem mutations, however it is not clear whether the mutations indeed occur more frequently or whether these tandem duplications are the results of strong selection pressure on these loci. Increased mutation rates could potentially be mediated by DNA repair pathways through homologous recombination, in particular by those pathways that act in regions with local repeats. This is an intriguing idea, as DNA repair through homologous recombination has been shown to be increased upon pathogen infection and in consequence pathogen infection could lead to increased mutation rates specifically in those genomic regions which encode the response to exactly this stress. With the advent of high-quality genome assemblies, we can now test this hypothesis by analysing the actual mutation rates in the notoriously-difficult-to-analyse R-gene clusters within mutation accumulation lines.

在植物中，参与抵抗生物胁迫的基因(所谓的R基因)的基因组组成涉及到正向(面向同一方向)串联复制的显著丰富。此外，长期以来，R基因簇因其在个体间的高度多样性而为人所知。综上所述，这表明R基因簇是通过正向串联突变的快速积累而进化的，然而，目前尚不清楚这些突变是否确实发生得更频繁，或者这些串联复制是否是这些座位上强大的选择压力的结果。DNA修复途径通过同源重组，特别是那些作用于局部重复区域的途径，可能会导致突变率的增加。这是一个有趣的想法，因为通过同源重组的DNA修复已被证明在病原体感染时增加，因此病原体感染可能导致突变率增加，特别是在编码对这种应激反应的基因组区域。随着高质量基因组组合的出现，我们现在可以通过分析突变累积线中出了名的难以分析的R基因簇的实际突变率来检验这一假设。