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Simple Speciation Technique for Arsenic and Antimony in Waste Material Recycling Processes

废物回收过程中砷和锑的简单形态分析技术

基本信息

批准号：
16550079
负责人：
OHKI Akira
金额：
$ 2.18万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

HPLC-hydride generation atomic fluorescence spectrometry (HPLC-HGAFS) is one of the arsenic speciation methods for biological samples, and the pretreatment of samples for the analytical method was studied. By use of various certified reference materials, the optimum conditions for the pretreatment (especially for the selection of extraction solvent) were examined. When the conventional method was used, the recovery of arsenic species except for arsenobetaine (AsB) was not good. The use of phosphoric acid solution as an extraction solvent resulted in the satisfactory recovery for not only AsB but also inorganic arsenicals (As(III) and As(V)), monomethylated arsenicals (MMA), and dimethylated arsenicals (DMA). Cryo trap-hydride generation atomic absorption spectrometry (CV-HGAAS), which is another method of arsenic speciation, was also tried, and it was found that CV-HGAAS gave about the same result that was obtained by HPLC-HGAFS. So far, there have been only a few studies about the com … More parison between the CT method and the HPLC method. In this study, we clarified the characteristics of both methods, and it is proved that the CT method has an advantage in the simplicity of operation, whereas the method can provide the same result that the HPLC method does. For the determination of total arsenic in the samples, microwave-assisted acid digestion was attempted, and the optimum conditions for the determination of arsenic and antimony were established (This result was submitted to a journal, Talanta, and accepted.) When dimethylarsenic acid (as a DMA) or AsB was added in a methane fermentation sludge, and the speciation of arsenic in the sludge was performed during the fermentation process. As the fermentation proceeded, DMA was methylated into AsB, and it was further transformed into trimethylarsine, which was transferred to gas phase. For the speciation of antimony, the fractional determination of trimethyl species and inorganic species was favorably carried out by use of CT-HGAAS. Less

高效液相色谱-氢化物发生原子荧光光谱法（hplc - hgas）是生物样品中砷形态分析方法之一，对该分析方法中样品的预处理进行了研究。采用各种标准物质，考察了最佳预处理条件（特别是萃取溶剂的选择）。在常规方法下，除砷甜菜碱（AsB）外，其它砷的回收率均不理想。使用磷酸溶液作为萃取溶剂，不仅对AsB，而且对无机砷（as （III）和as (V)）、单甲基化砷（MMA）和二甲基化砷（DMA）都有满意的回收率。还尝试了另一种砷形态分析方法——低温阱-氢化物发生原子吸收光谱法（CV-HGAAS），发现CV-HGAAS法与hplc - hgaas法所得结果基本一致。到目前为止，关于CT法和HPLC法的比较研究还比较少。在本研究中，我们明确了两种方法的特点，并证明了CT法在操作简单方面具有优势，而该方法可以提供与HPLC法相同的结果。为了测定样品中的总砷，尝试了微波辅助酸消化法，并建立了测定砷和锑的最佳条件（该结果已提交给《Talanta》杂志并被接受）。在甲烷发酵污泥中添加二甲基larsenic酸（DMA）或AsB，并在发酵过程中对污泥中的砷进行形态形成。随着发酵的进行，DMA被甲基化为AsB，并进一步转化为三甲基larsin，将其转移到气相。对于锑的形态，用CT-HGAAS可以很好地进行三甲基种和无机种的分式测定。少