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Application of Novel Separation Field, Polymer Aggregate, to Capillary Electrophoresis

新型分离场聚合物聚集体在毛细管电泳中的应用

基本信息

批准号：
10650799
负责人：
OHKI Akira
金额：
$ 2.11万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Some amphiphilic block-copolymers consisting of poly [(N-acylimino) ethylene] structure, which has hydrophilic blocks and hydrophobic blocks, form aggregates of 100 nm diameter in aqueous media. The polymer aggregate (AG) was added to the running solution of capillary electrophoresis (CE), and the further addition of a small amount of anionic surfactant, such as SDS, to provide negative charges onto the aggregate surface resulted in the functioning of AG as a pseudo-stationary phase (AG-CE). Thus, neutral compounds, such as phenols, were migrated in the order of lipophilicity. Compared to the conventional micellar electrokinetic chromatography by use of SDS (SDS-MEKC), the migration of nitrophenols was greatly retarded. Such anionic species are incorporated neither into AG nor into SDS micelles. The charge density of AG is much smaller than that of SDS micelles. Therefore, it appears that the migration of neutral species, which are incorporated in SDS micelles are smaller than that of the anionic species, whereas such a delay in the neutral species migration was not observed for the species incorporated in AG.Consequently, in AG-CE the migration of anionic species is smaller than that of neutral species incorporated in AG, unlike SDS-MEKC.To attain a novel micellar chromatography, the AG was added to the mobile phase in a reversed-phase chromatography (AG-LC). For the separation of naphthalenesulfonate derivatives, AG-LC showed a considerably different selectivity from that for the conventional micellar chromatography.In the future, the expression of further molecular recognition ability through the molecular modification of amphiphilic block-copolymers will hopefully lead to new applications of AG to CE and related chromatography technologies.

一些由聚[（n -酰基）乙烯]结构组成的两亲嵌段共聚物，具有亲水性嵌段和疏水性嵌段，在水介质中形成直径为100nm的聚集体。将聚合物聚集体（AG）加入到毛细管电泳（CE）运行液中，再加入少量阴离子表面活性剂（如SDS）使聚集体表面带负电荷，使AG作为伪固定相（AG-CE）发挥作用。因此，中性化合物，如酚类，是按照亲脂性的顺序迁移的。与传统的SDS- mekc胶束电色谱相比，硝基酚类化合物的迁移速度明显减慢。这些阴离子既不并入AG也不并入SDS胶束。AG胶束的电荷密度远小于SDS胶束。因此，在SDS胶束中加入的中性物质的迁移似乎比阴离子物质的迁移要小，而在AG胶束中加入的中性物质没有观察到这种迁移延迟。因此，与SDS-MEKC不同，AG- ce中阴离子物种的迁移小于AG中加入的中性物种的迁移。为了获得一种新型胶束色谱，将AG添加到反相色谱（AG- lc）的流动相中。AG-LC对萘磺酸盐衍生物的分离表现出与常规胶束色谱有明显不同的选择性。在未来，通过两亲性嵌段共聚物的分子修饰来表达进一步的分子识别能力，有望导致AG在CE和相关色谱技术上的新应用。