Search for mole cular probes from non-utilized marine resources of coral reef twilight zone

从珊瑚礁暮色带未利用海洋资源中寻找分子探针

基本信息

  • 批准号:
    16550145
  • 负责人:
  • 金额:
    $ 2.11万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2006
  • 项目状态:
    已结题

项目摘要

In order to discover new bioactive molecules, we examined metabolites of marine invertebrates collected from the coral reef twilight zone, where can be defined as depth range between 40 m and 150 m.A lipophilic extract of the sponge Suberites japonicus showed strong cytotoxicity,. After separation, a series of cytotoxins named sergamides A-F were obtained by analyzing their spectroscopic data and by making derivatives. As seragamide A inhibited cytokinc sis of cultured cells, it was suspected that the molecule might target the cytoskeletal protein actin. By using fluorescent-labeled 1 actin, we could confirm that seragamide A inhibited depolymerization of F-actin and facilitated polymerization of G-actin. This mode of action was similar to those of phalloidin and jaspamide. The sponge Dysidea cf. arenaria was found to contain moderate cytotoxins. By separating its extract, we obtained totally seven new diterpenes having spongian class skeleton. Their structures were elucidated by interpreting their NMR data and also by comparing the data with those reported. During this study we found that there exists variation on the spongian diterpenes with collection sites.A gorgonian coral Ellisella sp. was collected at Kunigami village and was found to contain new briarane class diterpenes. Some of them was shown to be cytokinesis inhibitors.In addition, we collected totally 44 specimens of marine invertebrates from the coral reef twilight zone along Okinawa Island, and obtained several known bioactive molecules after screening. As this research is still an on-going project, I hope we can isolate more new bioactive molecules from them.
为了发现新的生物活性分子,我们研究了从珊瑚礁黄昏地带采集的海洋无脊椎动物的代谢物,这里的深度范围在40米到150米之间。分离后,通过对其光谱数据的分析和衍生物的制备,得到了一系列的细胞毒素,命名为丝氨酰胺A-F。由于丝氨酰胺A抑制了培养细胞的细胞分裂,推测该分子可能针对细胞骨架蛋白肌动蛋白。通过荧光标记的肌动蛋白1,我们可以证实,丝氨酸胺A抑制了F-肌动蛋白的解聚,促进了G-肌动蛋白的聚合。这种作用模式类似于鬼臼草素和茉莉花胺。海绵Dyside a cf.海参中含有中等含量的细胞毒素。通过对其提取物的分离,共得到7个具有支链类骨架的新二萜类化合物。通过解释它们的核磁共振数据,并与文献报道的数据进行比较,确定了它们的结构。在本研究中,我们发现海绵二萜类化合物随采集点的不同而存在差异。在库尼加米村采集,发现含有新的布瑞烷类二萜类化合物。此外,我们从冲绳岛珊瑚礁暗带采集了44个海洋无脊椎动物标本,经过筛选得到了几个已知的生物活性分子。由于这项研究仍在进行中,我希望我们能从它们中分离出更多新的生物活性分子。

项目成果

期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Seragamide A-F, new actin-targeting depsipeptides from the sponge Suberites japonicus Thiele
Seragamide A-F,来自海绵 Suberites japonicus Thiele 的新型肌动蛋白靶向缩酚肽
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    C.Tanaka;J.Tanaka;R.F.Bolland;N.de Voogd;T.Higa
  • 通讯作者:
    T.Higa
海洋生物由来のアクチンを標的とする多様な分子
针对海洋生物肌动蛋白的多种分子
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    S.Osada;H.Kodama;田中淳一
  • 通讯作者:
    田中淳一
Briarane diterpenes of two species of octocorals, Ellisella sp. and Pteroeides sp.
两种八珊瑚的 Briarane 二萜,Ellisella sp。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    C.Tanaka;Y.Yamamoto;M.Otsuka;J.Tanaka;T.Ichiba;G.Marriott;R.Rachmat;T.Higa
  • 通讯作者:
    T.Higa
Seragamides A-F, new actin-targeting depsipeptides from the sponge Suberties japonicus Thiele
Seragamides A-F,来自海绵 Suberties japonicus Thiele 的新型肌动蛋白靶向缩酚肽
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    C.Tanaka;J.Tanaka;R.F.Bolland;G.Marriott;T.Higa
  • 通讯作者:
    T.Higa
Seragamides A-F, new actin-targeting depsipeptides from the sponge Suberites japonicus Thiele
  • DOI:
    10.1016/j.tet.2006.01.099
  • 发表时间:
    2006-04-10
  • 期刊:
  • 影响因子:
    2.1
  • 作者:
    Tanaka, C;Tanaka, J;Higa, T
  • 通讯作者:
    Higa, T
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

TANAKA Junichi其他文献

TANAKA Junichi的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('TANAKA Junichi', 18)}}的其他基金

Empirical research of sensory activities for lifelong learning support in multiple disabilities: Japanese-English comparative study
多重残疾终身学习支持的感官活动实证研究:日英比较研究
  • 批准号:
    18K02442
  • 财政年份:
    2018
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Search for antiviral molecules from coral reef organisms
从珊瑚礁生物中寻找抗病毒分子
  • 批准号:
    26350973
  • 财政年份:
    2014
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Study of Knowledge Instruction Theory based on Pathos in Hans Lipps
汉斯·利普斯基于悲情的知识传授理论研究
  • 批准号:
    26780434
  • 财政年份:
    2014
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Developing nurse-led nutrition improvement program based on local food culture and environment in rural Kenya
根据肯尼亚农村当地饮食文化和环境制定护士主导的营养改善计划
  • 批准号:
    26861984
  • 财政年份:
    2014
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Search and characterization of new helicase inhibitors and others from coral reef organisms
珊瑚礁生物中新型解旋酶抑制剂和其他抑制剂的搜索和表征
  • 批准号:
    22603007
  • 财政年份:
    2010
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A comparative study of age-related difference in the treatment effect of exogenous growth factors via endogenous regeneration
外源性生长因子通过内源性再生治疗效果随年龄变化的比较研究
  • 批准号:
    18591579
  • 财政年份:
    2006
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation on the variation of bioactive metabolites in coral reef organisms
珊瑚礁生物活性代谢物变化的研究
  • 批准号:
    10672109
  • 财政年份:
    1998
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Extension of almost periodic functions and the distribution of zeros
几乎周期函数的扩展和零点分布
  • 批准号:
    10640180
  • 财政年份:
    1998
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Relationship between Protestant Church and State in the 1980's German Democratic Republic
20世纪80年代德意志民主共和国新教教会与国家的关系
  • 批准号:
    05610028
  • 财政年份:
    1993
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

The Pleistocene history of coral reef growth and coral community changes in the Ryukyu Islands and Hawaii
琉球群岛和夏威夷的更新世珊瑚礁生长史和珊瑚群落变化
  • 批准号:
    24K07155
  • 财政年份:
    2024
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Increasing the sustainability and resilience of coral reef fisheries
提高珊瑚礁渔业的可持续性和复原力
  • 批准号:
    FL230100201
  • 财政年份:
    2024
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Australian Laureate Fellowships
Hawaiian Drowned Reefs: Climate variability and coral reef response to environmental change in the sub-tropical Pacific over the last 500 ky
夏威夷淹没的珊瑚礁:过去 500 年来气候变化和珊瑚礁对亚热带太平洋环境变化的响应
  • 批准号:
    NE/Y005503/1
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Research Grant
IRES Track II: Systems-based transdisciplinary approaches to coral reef science and conservation
IRES Track II:基于系统的跨学科方法进行珊瑚礁科学和保护
  • 批准号:
    2328190
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Standard Grant
2023 Mesophotic Coral Reef Ecosystems Gordon Research Conferences
2023 年中光珊瑚礁生态系统戈登研究会议
  • 批准号:
    2306373
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Standard Grant
Predicting spatial and temporal responses of herbivorous coral reef fishes to sediment runoff
预测草食性珊瑚礁鱼类对沉积物径流的时空响应
  • 批准号:
    2210070
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Standard Grant
Elucidation of Factors Contributing to the Formation of Proximate Coral Reef and Mangrove Environments in Micronesia
阐明密克罗尼西亚邻近珊瑚礁和红树林环境形成的因素
  • 批准号:
    23K12322
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
The Sick Man of the Sea: Using eDNA to Investigate the Impacts of Human Activities on the Biodiversity and Genetics of Coral Reef Assemblages
海中病夫:利用 eDNA 调查人类活动对珊瑚礁组合生物多样性和遗传学的影响
  • 批准号:
    2843356
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Studentship
CAREER: Species Interactions of Coral Reef Benthic Cyanobacterial Mats: Within-Mat Diversity Promotes Both Facilitation and Top-Down Control
职业:珊瑚礁底栖蓝藻垫的物种相互作用:垫内多样性促进便利化和自上而下的控制
  • 批准号:
    2239425
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Continuing Grant
Exploring the health and environmental drivers of coral reef bright spots
探索珊瑚礁亮点的健康和环境驱动因素
  • 批准号:
    2880800
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了