Investigation on the variation of bioactive metabolites in coral reef organisms

为了开展标题项目，我们在琉球群岛的珊瑚礁中采集了大量的Sarcophyton属软珊瑚标本和Stylotea和Dyside a属的海绵标本。每个样品都被提取，并用梯度高效液相色谱和核磁共振氢谱检查主要成分。我们从物种、季节和地理区域的角度对软珊瑚中的西膜类二萜进行了重点研究。结果表明，青冈中数量最多的是3种优势化学型，分别为灯盏花素、2R、7S、8S-石油醚和7-羟基-1，3，11-甲苯-20，8-内酯，以及几种稀有的化学型。轮枝链霉菌的主要化学类型为2R、7R、8R-肉毒杆菌，而埃伦贝氏链霉菌的主要化学类型为7，8-环氧基-1，3，11-棕榈烯。另外还调查了一些其他种，如：克氏链球藻、秀丽链球藻、链球藻、普氏链球藻、切波尼尔氏链球藻。大多数物种没有观察到季节变化，但有些物种可能存在地理变异。

In order to carry out the title project, we collected a large number of specimens of soft corals of the genus Sarcophyton and some sponges of the genera such as Stylotella and Dysidea in the coral reefs along the Ryukyu Islands. Each specimen was extracted and checked with gradient HPLC and ^1H NMR for major constituents. We focused on the cembranoid diterepenes in the soft corals with respect to species, seasons, and geographic regions. As a result, the most abundant species S.glaucum was found to be three dominant chemotypes containing emblide, 2R,7S,8S-sarcophytoxide, and 7-hydroxy-1,3,11-cembratrien-20,8- carbolactone together with several rare chemotypes. In case of S.trocheliophorum, the major chemotype was 2R,7R,8R-sarcophytoxide, while S.ehrenbergi was found to be 7,8-epoxy- 1,3,11-cembratriene. Some other species, S.crassocaule, S.elegans, S.infundibuliforme, S.pulchellum, S.cherbonnieri, were also investigated. Seasonal change was not observed for most species, however, geographic variation may exist in some species.