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Investigation of culturial physiology of tomato by analysis of phytohormone

通过植物激素分析研究番茄的栽培生理

基本信息

批准号：
12660021
负责人：
KOJIMA Kiyohide
金额：
$ 2.37万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660021/
关键词：
tomato phytohormone cytokinins indole-acetic acid apoplast symplast eibberellin abscisic acid IAA ABA LC-MS

项目摘要

As to indole-3-acetic acid(IAA), endogenous levels by liquid chromatography-mass spectrometry, diffusible levels by fluorometric detection and polar transport activity by radioactive IAA were determined in 3-month-old tomato plants. IAA concentration in the apoplast(AP, sap outside a cell) was higher than those in the symplast (SP, sap within a cell) in both upper and lower parts of stems, showing that IAA analysis of AP is important. Younger leaves exported much more diffusible IAA than older leaves. IAA concentration in the main roots was almost at the same level as in the stems. It is suggested that at sampling, the transport capacity of IAA is probably the limiting factor for IAA transport in stems, and the amount of polar IAA transport might be only 19% of the endogenous IAA amount in stems.The levels of IAA, gibberellic acid1 (GAl), trans-zeatin (Z) and trans-zeatin riboside(ZR) in seedless fruits of parthenocarpic tomato were analysed. Stage in 6cm diameter corresponding the middle of cell expansion phase were referred to expansion-stage, and stage in 8cm diameter corresponding the last of cell expansion phase were referred to MG-stage. IAA concentrations of the pericarps and partitions were higher in MG stage than in expansion-stage. In the partitions, IAA concentrations of SP were higher than those of AP in both expansion-and MG-stage. As to Z concentration, the SP of the partitions in expansion-stage had the highest and was 2.7 times than the AP, while in the pericarps were the same level in AP and SP. The ZR concentration in locule tissues in expansion-stage was the highest of all parts. The results suggest that the sites of synthesis may be the SP of partitions for IAA and Z, and locules for ZR.

采用液相色谱-质谱法测定3月龄番茄植株中吲哚-3-乙酸（IAA）的内源水平、荧光法测定其扩散水平和放射性IAA的极性运输活性。茎上部和下部的胞外体（AP，胞内液）中IAA浓度均高于胞内体（SP，胞内液）中IAA浓度，说明IAA分析具有重要意义。幼叶输出的扩散IAA比老叶多。主根的IAA浓度与茎的IAA浓度基本相同。因此，在取样时，IAA的运输能力可能是IAA在茎中运输的限制因素，极性IAA运输量可能仅为茎中内源IAA量的19%。对孤雌番茄无籽果实中IAA、赤霉素酸1 （GAl）、反式玉米蛋白(Z)和反式玉米蛋白核苷（ZR）含量进行了分析。细胞扩增中期直径为6cm的阶段称为扩增期，细胞扩增末期直径为8cm的阶段称为mg期。MG期果皮和分区的IAA浓度高于膨大期。在各分区中，SP在扩张期和mg期的IAA浓度均高于AP。在Z浓度方面，膨大期各分区的SP最高，为AP的2.7倍，果皮中AP与SP的含量相当。膨大期各组织的ZR浓度最高。结果表明，合成位点可能是IAA和Z分区的SP和ZR的小室。