Investigation of culturial physiology of tomato by analysis of phytohormone

至于吲哚-3-乙酸（IAA），内源水平的液相色谱-质谱法，扩散水平的荧光检测和极性运输活性的放射性IAA测定3个月大的番茄植株。茎上部和下部质外体（AP，细胞外汁液）中IAA含量均高于共质体（SP，细胞内汁液）中IAA含量，说明对AP进行IAA分析是重要的。幼叶比老叶输出更多的可扩散IAA。主根和茎中IAA含量基本相同。分析了单性结实番茄无核果中IAA、GA 1、Z和ZR的含量，结果表明：IAA的极性运输量可能是茎中IAA运输的限制因素，极性运输量可能仅为茎内源IAA的19%。细胞扩增中期直径为6cm的阶段称为扩增期，细胞扩增末期直径为8 cm的阶段称为MG期。MG期果皮和分液中IAA含量高于膨大期。在各分区中，无论在膨大期还是MG期，SP的IAA含量均高于AP。在Z含量上，膨大期各分区的SP含量最高，是AP的2.7倍，而果皮中AP和SP含量基本相同。结果表明，IAA和Z的合成位点可能是分区的SP，ZR的合成位点可能是小室。

As to indole-3-acetic acid(IAA), endogenous levels by liquid chromatography-mass spectrometry, diffusible levels by fluorometric detection and polar transport activity by radioactive IAA were determined in 3-month-old tomato plants. IAA concentration in the apoplast(AP, sap outside a cell) was higher than those in the symplast (SP, sap within a cell) in both upper and lower parts of stems, showing that IAA analysis of AP is important. Younger leaves exported much more diffusible IAA than older leaves. IAA concentration in the main roots was almost at the same level as in the stems. It is suggested that at sampling, the transport capacity of IAA is probably the limiting factor for IAA transport in stems, and the amount of polar IAA transport might be only 19% of the endogenous IAA amount in stems.The levels of IAA, gibberellic acid1 (GAl), trans-zeatin (Z) and trans-zeatin riboside(ZR) in seedless fruits of parthenocarpic tomato were analysed. Stage in 6cm diameter corresponding the middle of cell expansion phase were referred to expansion-stage, and stage in 8cm diameter corresponding the last of cell expansion phase were referred to MG-stage. IAA concentrations of the pericarps and partitions were higher in MG stage than in expansion-stage. In the partitions, IAA concentrations of SP were higher than those of AP in both expansion-and MG-stage. As to Z concentration, the SP of the partitions in expansion-stage had the highest and was 2.7 times than the AP, while in the pericarps were the same level in AP and SP. The ZR concentration in locule tissues in expansion-stage was the highest of all parts. The results suggest that the sites of synthesis may be the SP of partitions for IAA and Z, and locules for ZR.