Studies on mechanisms of novel systemic disease resistance induced through brassinosteroid-mediated signaling.

通过油菜素类固醇介导的信号诱导的新型系统抗病机制的研究。

• 批准号: 16580034
• 负责人: NAKASHITA Hideo
• 金额: $ 2.5万
• 依托单位: RIKEN
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580034/
• 关键词: brassinosteroid; disease resistance; Arabidopsis; 植物; 全身獲得抵抗性; サリチル酸

Brassinosteroids (BRs) have the ability to protect plants from various environmental stresses, such as drought, salinity, heat stress, and pathogens. Treatment of brassinolide (BL), considered to the most important BRs, induced systemic resistance against a broad range of pathogens in tobacco and rice plant. However, the mechanisms of brassinosteoid-mediated disease resistance (BDR) was not clarified. In this study, we analyzed the induction mechanism of BDR using Arabidopsis thaliana. BDR was effective in Arabidopsis and suppressed the growth of the virlent pathogen Pseudomonas syringae pv. tomato DC3000 (Pst). Analysis using brassinazole 2001, a specific inhibitor for BR biosynthesis, indicated that BDR plays part in defense response in Arabidopsis. Although NPR1 protein, a key component of systemic acquired resistance (SAR), was required for BDR development, BL treatment did not induce the expression of pathogenesis-related genes (PR-1, 2, and 5), SAR marker genes, nor the accumulation of salicylic acid. Spontaneous activation of SAR and BDR, exhibited additive protective effects against Pst, indicating that no cross talk exists between SAR- and BDR-signaling pathways. The PR gene expression by pathogen infection in BDR-induced plant was earlier than that in control plants, suggesting that BDR has some priming effect on plant immune system.

类甾醇（BRs）具有保护植物免受各种环境胁迫（如干旱、盐、热胁迫和病原体）的能力。芸苔素（BL）是烟草和水稻中最重要的一类抗病基因，它能诱导烟草和水稻产生对多种病原菌的系统抗性。然而，油菜素类骨质介导的抗病性（BDR）的机制尚不清楚。本研究以拟南芥为材料，分析了BDR的诱导机制。BDR在拟南芥中是有效的，并抑制了病原体假单胞菌的生长。番茄DC 3000（Pst.）利用BR生物合成的特异性抑制剂brassinazole 2001进行分析，表明BDR在拟南芥的防御反应中起作用。虽然NPR 1蛋白，系统获得性抗性（SAR）的关键组成部分，需要BDR的发展，BL治疗没有诱导的发病相关基因（PR-1，2和5），SAR标记基因的表达，也没有水杨酸的积累。自发激活的SAR和BDR，表现出对Pst的加性保护作用，表明SAR和BDR信号通路之间不存在串扰。BDR诱导的植株中PR基因的表达早于对照植株，表明BDR对植物免疫系统有一定的启动作用。

项目成果

Tiadinil, a novel plat activator, induces systemic acquired resistance in tobacco.

Tiadinil 是一种新型平台激活剂，可诱导烟草的系统获得性抗性。

• 发表时间: 2004
• 期刊: J.Pesticide Sci. 29
• 作者: Yasuda;M.;Nakashita;H.;Yoshida;S.
• 通讯作者: S.

Molecular Characterization of Two Highly Homologous Receptor-like Kinase Genes, RLK902 and RKL1, in Arabidopsis thaliana

• DOI: 10.1271/bbb.68.1935
• 发表时间: 2004-01
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Y. Tarutani;T. Morimoto;A. Sasaki;M. Yasuda;H. Nakashita;S. Yoshida;I. Yamaguchi;Yoshihito Suzuki

Thiadiazole carboxylic acid moiety of tiadinil, SV-03, induces systemic acquired resistance in tobacco without salicylic acid accumulation

• DOI: 10.1584/jpestics.31.329
• 发表时间: 2006-08
• 期刊: Journal of Pesticide Science
• 影响因子: 2.4
• 作者: M. Yasuda;M. Kusajima;M. Nakajima;K. Akutsu;T. Kudo;S. Yoshida;H. Nakashita

Induction of resistance against rice bacterial leaf blight by 3-chloro-l-methyl-1H-pyrazole-5-carboxylic acid

3-氯-1-甲基-1H-吡唑-5-甲酸诱导水稻白叶枯病抗性

• 发表时间: 2005
• 期刊: J. Pesticide Science
• 作者: Ishikawa;R;Nishioka M.
• 通讯作者: Nishioka M.

Foliar spray of validamaycin A or Validoxylamine A controls tomato Fusarium wilt

叶面喷施有效霉素 A 或有效羟胺 A 防治番茄枯萎病

• 发表时间: 2005
• 期刊: Phytopathology 95・10
• 作者: Suzuki;F.;H.Sawada;K.Azegami;K.Tsuchiya;Ryo Ishikawa
• 通讯作者: Ryo Ishikawa