Discovery of Food Microorganism : Fast and Effective Degradation of Intestinal Lipoperoxide and Hydrogen peroxide

本研究的目的是发现益生菌乳酸菌作为一种功能性食品，能够分解强脂过氧化物和过氧化氢，并加速其在肠道内形成的降解能力。2005年，我们开发了一种分离乳酸菌的分离培养基，该培养基含有脂过氧化物降解酶。此外，我们还分离到了具有较强过氧化氢降解能力的潜在菌株。2006年，我们从分离的乳酸菌中鉴定出有用的菌株，评估了过氧化氢分解的降解能力。在对乳酸菌进行基本鉴定和分组（《乳酸菌手册》，Asakura书店）后，我们共筛选出了1000株可能适用于功能食品的菌株，并从分离菌株中排除了潜在致病菌株。通过16sDNA序列和分类，对100株高脂过氧化物分解菌株进行了全鉴定。根据鉴定结果，它们被分类为植物乳杆菌科和戊糖Pediococcus。为了测量脂过氧化物和过氧化氢的降解活性，我们准备了乳酸杆菌标准GYP培养基，并在37℃条件下进行有氧培养，然后收获。以亚油酸为过氧化脂类化合物，过氧化异丙烯为模型化合物，测定其活性。此外，过氧化氢被用作亲水性底物。分离菌株中，植物乳杆菌Pan1-2对过氧化物的降解活性最高，戊糖Pediococcus Be-I对过氧化氢的降解活性最高。戊色Pediococcus Be-I可以清除过氧化异丙烯和过氧化氢。在L. plantarum Panl-2和P . pentosaceus Be-I反应产物过氧化丁基存在的情况下，两株静息细胞还原过氧化丁基为丁醇的能力相等。结果表明，菌株具有过氧化氢的2电子还原作用。少

The objectives of this research are to discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide and hydrogen peroxide breakdown, and to accelerate its degrading capability being formed in the intestine.In 2005, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of hydrogen peroxide. In 2006 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened a total ofl00 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 10 out 100 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and Phen … More otypic Analysis. As a result of the identification, they are classified as families of Lactobacillus plantarum and Pediococcus pentosaceus.For measuring degradation activity of lipoperoxide and hydrogen peroxide, we prepared for Lactobacillus Standard GYP medium, and conditioned with aerobic culture at 37□ and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide and Pediococcus pentosaceus Be-I performed the highest one of hydrogen peroxide. Pediococcus pentosaceus Be-I can scavenge both cumene peroxide and hydrogen peroxide.In presence of butyl peroxide for identifying reaction product of L. plantarum Panl-2 and P pentosaceus Be-I, the strains of resting cell showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strains have 2-electron reduction of peroxide. Less