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Discovery of Food Microorganism : Fast and Effective Degradation of Intestinal Lipoperoxide and Hydrogen peroxide

食品微生物的发现：快速有效降解肠道过氧化脂和过氧化氢

基本信息

批准号：
18580083
负责人：
NIIMURA Youichi
金额：
$ 2.3万
依托单位：
Tokyo University of Agriculture
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

The objectives of this research are to discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide and hydrogen peroxide breakdown, and to accelerate its degrading capability being formed in the intestine.In 2005, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of hydrogen peroxide. In 2006 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened a total ofl00 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 10 out 100 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and Phen … More otypic Analysis. As a result of the identification, they are classified as families of Lactobacillus plantarum and Pediococcus pentosaceus.For measuring degradation activity of lipoperoxide and hydrogen peroxide, we prepared for Lactobacillus Standard GYP medium, and conditioned with aerobic culture at 37□ and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide and Pediococcus pentosaceus Be-I performed the highest one of hydrogen peroxide. Pediococcus pentosaceus Be-I can scavenge both cumene peroxide and hydrogen peroxide.In presence of butyl peroxide for identifying reaction product of L. plantarum Panl-2 and P pentosaceus Be-I, the strains of resting cell showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strains have 2-electron reduction of peroxide. Less

本研究的目的是发现益生菌乳酸菌作为一种功能性食品，能够强力分解过氧化氢和过氧化氢，并加速其在肠道内形成的降解能力。2005年，我们开发了一种分离出具有过氧化氢降解酶的乳酸菌的分离培养基。此外，我们还分离出具有较强过氧化氢降解能力的潜在菌株。 2006年，我们从分离的乳酸菌中筛选出有用的菌株，评估其过氧化物分解机制的降解能力。经过对乳酸菌的基本鉴定和分组（《乳酸菌手册》，朝仓书店），共筛选出100株可能适用于功能性食品的菌株，并从分离菌株中排除了潜在的致病菌株。通过 16sDNA 序列和分类以及 Phen … More 异型分析，对 100 株进行高脂过氧化物分解的菌株中的 10 株进行了全面鉴定。鉴定结果，它们被分类为植物乳杆菌和戊糖片球菌科。为了测定过氧化脂和过氧化氢的降解活性，我们制备了乳杆菌标准GYP培养基，并在37℃下进行需氧培养，然后收获。我们使用亚油酸作为脂质过氧化物，并使用过氧化异丙苯作为模型化合物来测量其活性。此外，过氧化氢用作亲水底物。分离菌株中，植物乳杆菌Pan1-2对过氧化脂质的降解活性最高，戊糖片球菌Be-I对过氧化氢的降解活性最高。戊糖片球菌Be-I可以清除过氧化枯烯和过氧化氢。在丁基过氧化物存在下，用于鉴定植物乳杆菌Pal-2和戊糖片球菌Be-I的反应产物，静息细胞的菌株表现出相同的丁基过氧化物还原为丁醇的能力。结果表明，该菌株具有过氧化物的2电子还原作用。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Synechocystis DrgA protein functioning as nitroreducatse and ferric reductase is catalyzing the Fenton reaction

集胞藻 DrgA 蛋白作为硝基还原酶和铁还原酶催化芬顿反应

DOI：
发表时间：
2007
期刊：
The FEBS Journal vol. 274
影响因子：
0
作者：
Kouji;Takeda;Mayumi;Iizuka;Toshihiro;Watanabe;Junichi;Nakagawa;Shinji;Kawasaki;Youichi;Niimura
通讯作者：
Niimura

酸素と微生物の多様な生育

氧气和微生物的多样化生长

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Kousuke Nakamata;Tomokazu Kurita;M. Shah Alam Bhuiyan;Keisuke Sato;Yoichi Noda;and Koji Yoda;新村洋一
通讯作者：
新村洋一

Response of the microaerophilic Bifidobacterium species, B. boum and B. thermophilum, to oxygen.

微需氧双歧杆菌 B. boum 和 B. thermophilum 对氧气的反应。

DOI：
发表时间：
2006
期刊：
Applied and Environmental Microbiology 72巻10号
影响因子：
0
作者：
Yahata M.;H.Kunitake;K.Yasuda;K.Yamashita;H.Komatsu;R.Matsumoto;安田喜一・八幡昌紀・松本亮司・國武久登;國武久登・安田喜一・八幡昌紀・横山進・小松春喜;Shinji Kawasaki;Shinji Kawasaki;川崎信治;川崎信治;Shinji Kawasaki;Shinji Kawasaki
通讯作者：
Shinji Kawasaki

Subcellular Biochemistry Volume44

亚细胞生物化学第44卷