Discovery of Food Microorganism : Fast and Effective Degradation of Intestinal Lipoperoxide and Hydrogen peroxide

本研究的目的是发现益生乳酸菌作为一种功能性食品，能够强烈地分解过氧化脂质和过氧化氢，并加速其降解能力在肠道中形成。2005年，我们开发了一种分离培养基，分离出具有过氧化脂质降解酶的乳酸菌。此外，我们还筛选出了具有较强过氧化氢降解能力的潜在菌株。在2006年从分离的乳酸菌中鉴定有用的菌株时，我们评估了过氧化物分解机制的降解能力。在乳酸菌的基本鉴定和分组（乳酸菌手册，朝仓书店）之后，我们筛选了总共100株可能适用于功能性食品的菌株，并从分离的菌株中排除了潜在的致病菌株。通过16 sDNA测序和分类学鉴定，对100株高效过氧化脂质分解菌中的10株进行了全基因组鉴定 关于我们 表型分析为测定其对过氧化氢和过氧化脂质的降解活性，制备了乳杆菌GYP标准培养基，并在37 ℃下进行好氧条件培养，然后收获。我们利用亚油酸作为脂质过氧化物和枯烯过氧化物作为模型化合物来测量其活性。此外，使用过氧化氢作为亲水性底物。其中植物乳杆菌Pan 1 -2和戊糖片球菌Be-I对过氧化氢的降解活性最高。戊糖片球菌Be-I对过氧化异丙苯和过氧化氢都有较强的降解能力。Pantarum Panl-2和Pantentosaceus Be-I，静止细胞菌株对过氧化丁基的还原能力相当。结果表明，菌株具有过氧化物的2-电子还原。少

The objectives of this research are to discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide and hydrogen peroxide breakdown, and to accelerate its degrading capability being formed in the intestine.In 2005, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of hydrogen peroxide. In 2006 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened a total ofl00 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 10 out 100 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and Phen … More otypic Analysis. As a result of the identification, they are classified as families of Lactobacillus plantarum and Pediococcus pentosaceus.For measuring degradation activity of lipoperoxide and hydrogen peroxide, we prepared for Lactobacillus Standard GYP medium, and conditioned with aerobic culture at 37□ and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide and Pediococcus pentosaceus Be-I performed the highest one of hydrogen peroxide. Pediococcus pentosaceus Be-I can scavenge both cumene peroxide and hydrogen peroxide.In presence of butyl peroxide for identifying reaction product of L. plantarum Panl-2 and P pentosaceus Be-I, the strains of resting cell showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strains have 2-electron reduction of peroxide. Less