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Innate immunity and protective immune responses in yellowtail induced by liposome-type Lactococcus garvieae vaccine

脂质体型格氏乳球菌疫苗诱导黄尾鱼的先天免疫和保护性免疫反应

基本信息

批准号：
16580254
负责人：
KODAMA Hiroshi
金额：
$ 2.3万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Intensive fish farming has become a key industry in recent decades. With the increasing scale of aquaculture, fish are now reared at high population densities. Mass mortality occurs if infectious agents are introduced into the farm, causing great financial loss. Difficulty in control of disease arises from the limited effectiveness of chemotherapy, and from asymptomatic carriage of the disease, leading to outbreaks of disease when affected fish stocks are stressed. Lactococcus garvieae infection in the yellowtail is a prevalent bacterial disease in Japan. The mucosal tissues of the body of fish constitute an enormous surface area, and many infectious organisms invade through or colonise mucosal tissues, especially the intestinal tract. These pathogens recognize the carbohydrate structure on the surface of cells as a receptor for binding. Glycosphingolipids (GSL) are ubiquitous components of the plasma membrane of cells. The present study was undertaken to demonstrate the adherence of L.garvieae to GSL of the yellowtail. Lactococcus garvieae adhered to ganglioside bands purified from the intestinal mucous membrane and brain having similar mobility to GM_3 ganglioside standard, according to thin layer chromatography. Adherence of the bacteria was blocked by pretreatment of the intestinal tract preparation with glycosidase in a dose-dependent manner, implying indicating that ganglioside acts as a binding receptor of L.garvieae in the yellowtail intestinal tract.

近几十年来，集约化鱼类养殖已成为一个关键产业。随着水产养殖规模的不断扩大，鱼类养殖密度越来越高。如果传染源被引入农场，就会造成大规模死亡，造成巨大的经济损失。控制疾病的困难源于化疗的有效性有限，以及疾病的无症状携带，当受影响的鱼类种群受到压力时，导致疾病的爆发。黄尾鱼的加氏乳球菌感染是日本一种流行的细菌性疾病。鱼体的粘膜组织构成了巨大的表面积，许多传染性微生物通过粘膜组织特别是肠道侵入或定植。这些病原体将细胞表面的碳水化合物结构识别为结合受体。鞘糖脂 (GSL) 是细胞质膜中普遍存在的成分。本研究旨在证明 L.garvieae 对黄尾鱼 GSL 的依从性。根据薄层色谱法，加维乳球菌粘附在从肠粘膜和脑中纯化的神经节苷脂条带上，其迁移率与GM_3神经节苷脂标准品具有相似的迁移率。用糖苷酶对肠道制剂进行预处理，以剂量依赖的方式阻断细菌的粘附，这表明神经节苷脂在黄尾鱼肠道中充当了加氏乳杆菌的结合受体。