Oncogenicity of fish herpesvirus and protective immune mechanisms of fish

研究了3种沙门疱疹病毒（H-83株、马苏Oncorhynchus masou病毒（OMV）和沙门疱疹病毒）的生物学、血清学和遗传关系。菌株H-83对马苏鲑鱼和虹鳟鱼鱼苗有致病性，但对鲈鱼不敏感。鱼产生低效价的病毒中和抗体，并观察到二次免疫应答的表达。感染了OMV的银鲑苗在口腔内和周围产生了肿瘤。病毒再分离结果表明肝脏是OMV感染的靶器官。交叉酶联免疫吸附试验和交叉中和试验表明，菌株H-83与OMV血清学相似，但与沙门氏菌有差异。DNA-DNA杂交试验还表明，菌株H-83与OMV DNA之间的同源DNA序列比它们与沙门氏菌之间的同源DNA序列多得多。菌株H-83 DNA的限制性内切酶切割模式与OMV相似，但与沙门氏菌不同。在体外传代33次以上后，一些片段出现或消失，但沙门氏菌的DNA片段在传代过程中没有发生变化。前两种病毒在培养皿中传代后毒性下降。

Biological, serological and genetical relationships of three salmonid herpesviruses (strain H-83, Oncorhynchus masou virus (OMV) and Herpesvirus salmonis) were investigated.The strain H-83 was pathogenic to masu salmon and rainbow trout fry, however, yearing fish were not sensitive. The fish produced virus-neutralizing antibody with low titers, and the expression of secondary immune response was observed.Coho salmon fry infected with OMV developed tumors in and around the oral cavity. The results of virus re-isolation indicated that the liver was the target organ of OMV infection.Cross enzyme-linked immunosorbent assay and cross neutralization test revealed that strain H-83 and OMV were serologically similar but different from H.salmonis.DNA-DNA hybridization test also suggested the presence of many more homologous DNA sequences between strain H-83 and OMV DNAs than between them and H. salmonis.Restriction endonuclease cleavage pattern of strain H-83 DNA was similar to that of OMV but distinct from that of H. salmonis. After passages of the viruses for more than 33 times in vitro, some fragments appeared or disappeared, however, no DNA fragment of H. salmonis changed during the passages. The former two viruses showed a decrease in virulence in fry after passages.