Oncogenicity of fish herpesvirus and protective immune mechanisms of fish

对三种鲑鱼疱疹病毒（H-83株、马苏虹鳟病毒（OMV）和鲑鱼疱疹病毒）的生物学、血清学和遗传关系进行了研究。H-83株对马苏鲑鱼和虹鳟鱼苗有致病性，但向鱼不敏感。鱼产生低滴度的病毒中和抗体，并观察到二次免疫反应的表达。感染OMV的银鲑鱼苗在口腔内和周围形成肿瘤。病毒重分离结果表明，肝脏是OMV感染的靶器官。交叉酶联免疫吸附试验和交叉中和试验表明，菌株H-83和OMV在血清学上相似，但与H.salmonis不同。DNA-DNA杂交试验也表明菌株H-83和OMV DNA之间存在比它们与H.salmonis之间更多的同源DNA序列。H-83菌株的限制性内切酶裂解模式DNA 与 OMV 相似，但与鲑鱼不同。病毒在体外传代超过33次后，一些片段出现或消失，但鲑鱼的DNA片段在传代过程中没有发生变化。前两种病毒在传代后在鱼苗中表现出毒力下降。

Biological, serological and genetical relationships of three salmonid herpesviruses (strain H-83, Oncorhynchus masou virus (OMV) and Herpesvirus salmonis) were investigated.The strain H-83 was pathogenic to masu salmon and rainbow trout fry, however, yearing fish were not sensitive. The fish produced virus-neutralizing antibody with low titers, and the expression of secondary immune response was observed.Coho salmon fry infected with OMV developed tumors in and around the oral cavity. The results of virus re-isolation indicated that the liver was the target organ of OMV infection.Cross enzyme-linked immunosorbent assay and cross neutralization test revealed that strain H-83 and OMV were serologically similar but different from H.salmonis.DNA-DNA hybridization test also suggested the presence of many more homologous DNA sequences between strain H-83 and OMV DNAs than between them and H. salmonis.Restriction endonuclease cleavage pattern of strain H-83 DNA was similar to that of OMV but distinct from that of H. salmonis. After passages of the viruses for more than 33 times in vitro, some fragments appeared or disappeared, however, no DNA fragment of H. salmonis changed during the passages. The former two viruses showed a decrease in virulence in fry after passages.