Oncogenicity of fish herpesvirus and protective immune mechanisms of fish

鱼类疱疹病毒的致癌性及鱼类的保护性免疫机制

• 批准号: 61560350
• 负责人: KODAMA Hiroshi
• 金额: $ 0.83万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560350/
• 关键词: salmonids; herpesvirus; pathogenicity; tumor; DNA-DNA hybridization; 制限酵素切断パターン; サケ科魚ヘルペスウイルス; H-83株; OMV; ヘルペスウイルスサルモニス; 腫瘍原性

Biological, serological and genetical relationships of three salmonid herpesviruses (strain H-83, Oncorhynchus masou virus (OMV) and Herpesvirus salmonis) were investigated.The strain H-83 was pathogenic to masu salmon and rainbow trout fry, however, yearing fish were not sensitive. The fish produced virus-neutralizing antibody with low titers, and the expression of secondary immune response was observed.Coho salmon fry infected with OMV developed tumors in and around the oral cavity. The results of virus re-isolation indicated that the liver was the target organ of OMV infection.Cross enzyme-linked immunosorbent assay and cross neutralization test revealed that strain H-83 and OMV were serologically similar but different from H.salmonis.DNA-DNA hybridization test also suggested the presence of many more homologous DNA sequences between strain H-83 and OMV DNAs than between them and H. salmonis.Restriction endonuclease cleavage pattern of strain H-83 DNA was similar to that of OMV but distinct from that of H. salmonis. After passages of the viruses for more than 33 times in vitro, some fragments appeared or disappeared, however, no DNA fragment of H. salmonis changed during the passages. The former two viruses showed a decrease in virulence in fry after passages.

研究了三种鲑鱼疱疹病毒（菌株H-83，Oncorhynchus masou病毒（OMV）和疱疹病毒鲑鱼）的生物学，血清学和遗传关系。但是，H-83菌株对Masu Salmon和Rainbow Trout Fry具有致病性，但是，一年一度的鱼都不敏感。该鱼类产生了低滴度的病毒中和抗体，并观察到了次级免疫反应的表达。被OMV感染的Coho鲑鱼在口腔内和周围发生了肿瘤。 The results of virus re-isolation indicated that the liver was the target organ of OMV infection.Cross enzyme-linked immunosorbent assay and cross neutralization test revealed that strain H-83 and OMV were serologically similar but different from H.salmonis.DNA-DNA hybridization test also suggested the presence of many more homologous DNA sequences between strain H-83 and OMV DNAs than between them and H. Salmonis。限速性核酸内切酶H-83 DNA的裂解模式与OMV相似，但与H. salmonis不同。在体外经过超过33次的病毒通过后，一些片段出现或消失了，但是，在通道期间，鲑鱼的DNA片段没有变化。前两个病毒在通道后的炸毒力降低。

项目成果

児玉洋: ウイルス. 37. 169-177 (1987)

儿玉浩：病毒。37. 169-177 (1987)

児玉洋: "新編獣医微生物学" 養覧堂,

Hiroshi Kodama：《兽医微生物学新版》Yorando，

Hayashi,Y.: Japanese Journal of Veterinary Science. 49. 251-260 (1987)

Hayashi,Y.：日本兽医科学杂志。

児玉洋: "水生動物医学各論" 日本小動物獣医師会, 248 (1987)

Hiroshi Kodama：《水生动物医学》日本小动物兽医协会，248（1987）

Hayashi,Y.: Japanese Journal of Veterinary Science. 48. 915-924 (1986)

Hayashi,Y.：日本兽医科学杂志。