Production of immune regulatory humoral factor in fish and role in protective immune reaclions

鱼类免疫调节体液因子的产生及其在保护性免疫反应中的作用

• 批准号: 05660348
• 负责人: KODAMA Hiroshi
• 金额: $ 1.34万
• 依托单位: University of Osaka Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660348/
• 关键词: hematopoietic cell-stimulating factor; M-CSF; macrophage; botulinum C_2 toxin; actin; CRP; acute phase protein; SAP; サイトカイン; マクロファージコロニー刺激因子; ADPリボシル化; 増殖刺激因子; 頭腎細胞; マクロファージコロニー; ニジマス; 血清

Cytokines are very important humoral factors that regulate immune responses of animals. In fish, however little is known on the nature of cytokines and role in protective immune responses. In the present study, the production and the nature of hematopoietic cell-stimulatinf factors including M-CSP were analyzed. Macrophage-colonies developed in semi-solid agar culture of head kidney cells in the presence of rainbow trout serum. Inoculation of LPS, Freund's complete adjuvant and MDP in trout enhanced colony-forming activity of serum. Treatment of macrophages with botulinum C_2 toxin induced ADP-ribosylation of intracellular actins. and phagocytic activity of macrophages was inhibited.CRP and other acute-phase proteins have important role in protective immune responses and in regulation of physiological conditions in animals. Regulatory functions of acute phase proteins in immune responses of rainbow trout were examined in the present study. Amino acid analysis of CRP and SAP purified from trout serum showed similar amino acid sequences to that of other animal species. Electron microscopy of CRP and SAP showed pentameric structures that is characteristic of pentraxins. In serum collected from trout under inflammation conditions showed increased concentrations of CRP and 43 kDa neutral serum protein.

细胞因子是调节动物免疫反应的重要体液因子。然而，在鱼类中，对细胞因子的性质和在保护性免疫反应中的作用知之甚少。本研究分析了包括M-CSP在内的造血细胞刺激因子的产生和性质。虹鳟鱼血清存在下，半固体琼脂培养头肾细胞形成巨噬细胞菌落。在鳟鱼体内接种脂多糖、弗氏完全佐剂和MDP可增强血清的菌落形成活性。肉毒杆菌C_2毒素治疗巨噬细胞诱导胞内肌动蛋白adp核糖基化。并抑制巨噬细胞的吞噬活性。CRP等急性期蛋白在动物的保护性免疫反应和生理调节中具有重要作用。研究了虹鳟鱼急性期蛋白在免疫应答中的调控作用。从鳟鱼血清中纯化的CRP和SAP的氨基酸序列与其他动物相似。电镜下CRP和SAP显示戊烷素的五聚体结构。在炎症条件下采集的鳟鱼血清中，CRP和43 kDa中性血清蛋白浓度升高。

项目成果

Kodama,H.,Baba,T.and Ohishi,I.: "Inhibition of phagoiytosis by rainbow trout macrophages by botulinum C_2 toxin and its trypsinized component II" Developmental and Comparative Immunology. 18. 389-395 (1994)

Kodama,H.、Baba,T. 和 Ohishi,I.：“肉毒杆菌 C_2 毒素及其胰蛋白酶化成分 II 对虹鳟巨噬细胞的吞噬作用的抑制”发育和比较免疫学。

Kodama,H.and Ohishi,I: "ADP-ribosylation of rainbow trout actin by botulinum C_2 toxin" Infection and Immunity. 61. 5392-5393 (1993)

Kodama,H. 和 Ohishi,I：“肉毒杆菌 C_2 毒素对虹鳟鱼肌动蛋白的 ADP-核糖基化”感染和免疫。

Murata.M., Sugimoto, C., Kodama, H.and Onuma.M.: "Analysis of changes in serum proteins of rainbow trout treated with inflammation inducers or high temperature by using two-dimentional gel electrophoresis." Proceedings of Japanese Society of Animal Bioche

Murata.M.、Sugimoto, C.、Kodama, H. 和 Onuma.M.：“利用二维凝胶电泳分析经炎症诱导剂或高温处理的虹鳟鱼血清蛋白的变化。”

Murata,M.,Onuma,M.and Kodama,H.: "Isolation and characterization of rainbow trout serum amyloid P component(SAP)" Journal of Veterinary Medical Science. 56. 661-665 (1994)

Murata,M.、Onuma,M. 和 Kodama,H.：“虹鳟鱼血清淀粉样蛋白 P 成分 (SAP) 的分离和表征”兽医医学杂志。

Murata, M., Onuma.M.and Kodama, H.: "Isolation and characterization of rainbow trout (Oncorhynchus mykiss) serum amyloid P component (SAP)." Journal of Veterinary Medical Science.56 (4). 661-665 (1994)

Murata, M.、Onuma.M. 和 Kodama, H.：“虹鳟鱼 (Oncorhynchus mykiss) 血清淀粉样蛋白 P 成分 (SAP) 的分离和表征。”