Production of immune regulatory humoral factor in fish and role in protective immune reaclions

细胞因子是调节动物免疫反应的重要体液因子。然而，在鱼类中，对细胞因子的性质和在保护性免疫反应中的作用知之甚少。本研究分析了包括M-CSP在内的造血细胞刺激因子的产生和性质。虹鳟鱼血清存在下，半固体琼脂培养头肾细胞形成巨噬细胞菌落。在鳟鱼体内接种脂多糖、弗氏完全佐剂和MDP可增强血清的菌落形成活性。肉毒杆菌C_2毒素治疗巨噬细胞诱导胞内肌动蛋白adp核糖基化。并抑制巨噬细胞的吞噬活性。CRP等急性期蛋白在动物的保护性免疫反应和生理调节中具有重要作用。研究了虹鳟鱼急性期蛋白在免疫应答中的调控作用。从鳟鱼血清中纯化的CRP和SAP的氨基酸序列与其他动物相似。电镜下CRP和SAP显示戊烷素的五聚体结构。在炎症条件下采集的鳟鱼血清中，CRP和43 kDa中性血清蛋白浓度升高。

Cytokines are very important humoral factors that regulate immune responses of animals. In fish, however little is known on the nature of cytokines and role in protective immune responses. In the present study, the production and the nature of hematopoietic cell-stimulatinf factors including M-CSP were analyzed. Macrophage-colonies developed in semi-solid agar culture of head kidney cells in the presence of rainbow trout serum. Inoculation of LPS, Freund's complete adjuvant and MDP in trout enhanced colony-forming activity of serum. Treatment of macrophages with botulinum C_2 toxin induced ADP-ribosylation of intracellular actins. and phagocytic activity of macrophages was inhibited.CRP and other acute-phase proteins have important role in protective immune responses and in regulation of physiological conditions in animals. Regulatory functions of acute phase proteins in immune responses of rainbow trout were examined in the present study. Amino acid analysis of CRP and SAP purified from trout serum showed similar amino acid sequences to that of other animal species. Electron microscopy of CRP and SAP showed pentameric structures that is characteristic of pentraxins. In serum collected from trout under inflammation conditions showed increased concentrations of CRP and 43 kDa neutral serum protein.