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A control structure of a nervous system synapse morphosis by a cell adhesion molecule.

细胞粘附分子控制神经系统突触形态的结构。

基本信息

批准号：
16590142
负责人：
MIZOGUCHI Akira
金额：
$ 2.24万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Nectins are Ca^<2+>-independent immunoglobulin-like cell-cell adhesion molecules and comprise a family of four members. At the mossy fiber terminals of hippocampus, nectin-1 and nectin-3 localize at the presynaptic and postsynaptic sides of synaptic junctions, respectively, and their trans-interaction plays a role in formation of synapses in cooperation with N-cadherin. Nectins are associated with the actin cytoskeleton through afadin, a nectin- and actin filament-binding protein. Inhibition of nectin-1-and nectin-3-based adhesion by in nectin-1 -/- mice resulted in the abnormal formation of synapses (Honda et al., 2006). Taken together, it is likely that nectins are involved in the formation of synapses in cooperation with N-cadherin. This role of nectins is consistent with the finding that mutations in the nectin-1 gene are responsible for cleft lip/palate-ectodermal dysplasia, which is characterized by mental retardation in addition to ectodermal dysplasia.For identification of a novel molecule that have cell-aggregation activity and is involved in synapse formation, we made a subtraction library for developmental hippocampus (P10-P5). This library was transfected into fibroblasts and the transfected cells were performed with cell-aggregation assay. We identified a voltage-gated K^+ channel as a cell adhesion molecule (Kimura et al., in preparation for submission). Furthermore, we also found that aquaproin 4, a water channel, has cell aggregation activity (Hiroaki et al. 2006).

Nectin是Ca^<2+>-非依赖性免疫球蛋白样细胞-细胞粘附分子，并且包括四个成员的家族。在海马苔藓纤维终末，nectin-1和nectin-3分别定位于突触连接的突触前和突触后两侧，它们之间的相互作用与N-cadherin共同参与突触的形成。连接蛋白通过afadin与肌动蛋白细胞骨架结合，afadin是一种连接蛋白和肌动蛋白结合蛋白。在nectin-1 -/-小鼠中抑制基于nectin-1-和nectin-3-的粘附导致突触的异常形成（本田等人，2006年）。两者合计，这是可能的，nectin参与突触的形成与N-钙粘蛋白的合作。Nectin-1基因突变与唇腭裂-外胚层发育不良（除了外胚层发育不良外，还表现为智力低下）的发生有关。为了鉴定一种新的具有细胞聚集活性并参与突触形成的分子，我们构建了发育海马（P10-P5）消减文库。将该文库转染成纤维细胞，并对转染的细胞进行细胞聚集试验。我们鉴定了一种电压门控K^+通道作为细胞粘附分子（Kimura et al.，准备提交）。此外，我们还发现水通道aquaproin 4具有细胞聚集活性（Hiroaki et al.2006）。

项目成果

期刊论文数量（50）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nectin-like molecule-1/TSLL1/SynCAM3 : a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-functional contact sites of presynaptic nerve terminals, axons and glia cell processes.

Nectin-like molecular-1/TSLL1/SynCAM3：一种神经组织特异性免疫球蛋白样细胞-细胞粘附分子，定位于突触前神经末梢、轴突和神经胶质细胞突起的非功能性接触位点。

DOI：
发表时间：
2005
期刊：
J Cell Sci. 118・Pt6
影响因子：
0
作者：
Kakunaga S;Ikeda W;Itoh S;Deguchi-Tawarada M;Ohtsuka T;Mizoguchi A;Takai Y.
通讯作者：
Takai Y.

Characterization and function of MYPT2, a target subunit of myosin phosphatase in heart

DOI：
10.1016/j.cellsig.2005.11.001
发表时间：
2006-09-01
期刊：
CELLULAR SIGNALLING
影响因子：
4.8
作者：
Okamoto, Ryuji;Kato, Takaaki;Ito, Masaaki
通讯作者：
Ito, Masaaki

Neurite outgrowth from hippocampal neurons is promoted by choroid plexus ependymal cells in vitro.

体外脉络丛室管膜细胞促进海马神经元的神经突生长。

DOI：
发表时间：
2004
期刊：
J. Neurocytol. 33
影响因子：
0
作者：
Kimura K.;Ide C.;et al.
通讯作者：
et al.

Involvement of nectins in the formation of puncta adherentia junctions and the mossy fiber trajectory in the mouse hippocampus

DOI：
10.1016/j.mcn.2005.10.002
发表时间：
2006-02-01
期刊：
MOLECULAR AND CELLULAR NEUROSCIENCE
影响因子：
3.5
作者：
Honda, T;Sakisaka, T;Takai, Y
通讯作者：
Takai, Y

Differential localization and regulation of stargazin-like protein, gemma-8 and stargazin in the plasma membrane of hippocampal and cortical neurons.

海马和皮质神经元质膜中stargazin 样蛋白、gemma-8 和stargazin 的差异定位和调节。

DOI：
发表时间：
2006
期刊：
Neurosci Res. 55-1
影响因子：
0
作者：
Inamura M;Itakura M;Okamuto H;Hoka S;Mizoguchi A;Fukazawa Y;Shigemoto R;Yamamori S;Takahashi M.
通讯作者：
Takahashi M.