Study on the physiological function of bombyxin

家蚕素的生理功能研究

• 批准号: 04640655
• 负责人: MIZOGUCHI Akira
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640655/
• 关键词: Bombyx mori; Insulin-like peptide; Bombyxin; Fluoroimmunoassay; Metamorphosis; Metabolism; Trehalase; glycogen; 分泌動態; 時間分解蛍光免疫測定法; 糖代謝; 脂質代謝; 時間分解螢光免疫測定法; 血糖低下作用; トレハロース; 微量定量法; モノクローナル抗体

1. We produced many kinds of mouse monoclonal antibodies against synthetic bombyxin-II and developed a time-resolved fluoroimmunoassay (TR-FIA) for bombyxin. This assay method is superior to the conventional radioimmunoassay in sensitivity, simplicity, safety and time-saving.2. Using TR-FIA,we examined the changes in bombyxin titer in hemolymph during larva-pupa-adult development of the silkmoth, Bombyx mori. The titer was low in the larval stage, while high in the early and middle pupal stages. A steep rise in the titer was observed immediately after eclosion only in males. Hemolymph lipid also increased after eclosion only in males, suggesting a possibility that bombyxin is involved in the regulation of lipid metabolism.3. We investigated the physiological function of bombyxin in Bombyx larvae and obtained the following results.1) Bombyxin has a hypotrehalosemic activity.2) Bombyxin increases the trehalase activity in the midgut and integument (including muscles).3) Bombyxin has no effect on trehalose synthase activity in the fat body.4) Bombyxin decreases glycogen content in the fat body.These results suggested that bombyxin regulates sugar and lipid metabolism and that bombyxin plays an important role (s) in the process of metamorphosis.

1. 我们制备了多种抗合成蚕蛹毒素的小鼠单克隆抗体，并建立了一种时间分辨荧光免疫法（TR-FIA）检测蚕蛹毒素。该方法具有灵敏度高、操作简便、安全、省时等优点。利用TR-FIA检测了家蚕幼虫-蛹-成虫发育过程中血淋巴中家蚕素滴度的变化。幼虫期滴度低，蛹期前期和中期滴度高。只在雄虫羽化后立即观察到滴度急剧上升。羽化后血淋巴脂质也仅在雄性中增加，提示蚕蛹毒素可能参与了脂质代谢的调节。我们对家蚕幼虫中蚕蛹素的生理功能进行了研究，得到以下结果：蚕蛹素具有低海藻活性。2)蚕贝素增加了中肠和被膜（包括肌肉）的海藻化酶活性。3)蚕贝素对脂肪体海藻糖合成酶活性无影响。4)蚕豆素降低脂肪体中糖原含量。这些结果表明，蚕豆素调节糖和脂质代谢，在变态过程中起重要作用。

项目成果

三枝 弘尚: "Changes in the titer of bombyxin-immunoreactive material in hemolymph during the postembryonic development of the silkmoth Bombyx mori." Development Growth and Differentiation. 34. 595-605 (1992)

Hironaka Saegusa：“家蚕胚胎后发育过程中血淋巴中蚕素免疫反应物质滴度的变化。” 34. 595-605 (1992)。

J.-d.Dai, et al.: "Immunoreactivity of neurosecretorygranules in the brain-retrocerebral complex of Manduca sexta to heterologous antibodies against Bombyx prothoracicotropic hormone and bombyxin." Invertebrate Reproduction and Development. 26. 187-196 (1

J.-d.Dai 等人：“天蛾脑-脑后复合体中的神经分泌颗粒对针对家蚕促胸腺激素和家蚕素的异源抗体的免疫反应性。”

溝口 明(分担): "Perspectives in Comparative Endocrinology(K.G.Davey他編)" Research Journals Division of the National Research council of Canada.(印刷中), (1994)

Akira Mizoguchi（撰稿人）：“比较内分泌学的观点（由 K.G. Davey 等人编辑）”加拿大国家研究委员会研究期刊部（正在出版），（1994 年）。

三枝 弘尚: "Change in the titer of bombyxin-immunoreactive material in hemolymph during the postembryonic development of the silkmoth Bombyx mori." Development Growth and Differentiation. 34. 595-605 (1992)

Hironaka Saegusa：“家蚕胚胎后发育过程中血淋巴中家蚕素免疫反应物质滴度的变化。” 34. 595-605 (1992)。

A.Mizoguchi: "Distribution and function of bombyxin.In : Perspectivesin Comparative Endocrinology (eds : K.G.Davey et al.), pp.215-219, National Research Council of Canada, 1994"

A.Mizoguchi：“蚕素的分布和功能。In：比较内分泌学的观点（编辑：K.G.Davey 等），第 215-219 页，加拿大国家研究委员会，1994 年”