Molecularpharmacological analysis of mechanosensitive cation channels in vascular endothelial cells.

1)小鼠PKD2L1(TRPP2L1)的鉴定我们克隆了小鼠PKD2L1基因，它是Trp通道家族的成员。2)TRPC的功能分析利用过表达的转基因小鼠，阐明神经元限制性沉默因子(NRSF)在TRPC1表达中的调节作用(Ohba等人，2005)。电压依赖性钙通道的功能作为阳离子通道家族成员，我们研究了电压依赖性钙通道的功能，分析了电压依赖性钙通道在伤害性神经传递中的作用(Murakami等人，《神经科学通讯》，2004；Murakami等，Brain Res.，2004)。β3亚单位通过增加IP3产量对胰岛素分泌的重要性已被证明(Berggren等人，2004年)。评价了全氟辛烷磺酸(全氟辛烷磺酸)和全氟辛酸(全氟辛酸)对L类钙通道的抑制作用(Harada等人，2005年)。我们阐明了血栓素A2和前列腺素F2α与炎症性心动过速的关系(Takayama等人，2005年)。

1)Identification of murine PKD2L1 (TRPP2L1)We cloned murine PKD2L1 gene, which is a member of TRP channel family. PKD2L1 forms functional cation channels on the plasma membrane by interacting with PKD1 (Murakami et al., J.Biol.Chem., 2005).2)Functional analysis of TRPCUsing overexpression transgenic mouse, regulatory role of neuron-restrictive silencing factor (NRSF) in expression of TRPC1 was clarified (Ohba et al., 2005).Regulatory effect of unoprostone on store operated Ca^<2+> entry (SOCE) in human aortic smooth muscle cells was investigated (Watanabe et al., 2005).3)Functions of voltage-dependent calcium channelsAs a cation channel family member, we investigated functions of voltage-dependent calcium channels.Roles of voltage- dependent calcium channels in nociceptive neurotransmission were analyzed (Murakami et al., Neuroscience letters, 2004, Murakami et al., Brain Res., 2004). Importance of β3 subunit on insulin secretion through increase in IP3 production was proved (Berggren, et al., 2004). Inhibitory effects of perfuorooctane sulfonate (PFOS) and perfuorooctanoate (PFOA) on L-type calcium channels were evaluated (Harada et al., 2005). We clarified involvement of Thromboxane A2 and prostaglandin F2α in inflammatory tachycardia (Takayama et al., 2005).