Analyses of Roles of SOD1 in Protection against Photoaging and Carcinogenesis Using SOD1-deficient Mice

使用 SOD1 缺陷小鼠分析 SOD1 在防止光老化和致癌作用中的作用

基本信息

  • 批准号:
    16590238
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2005
  • 项目状态:
    已结题

项目摘要

Singlet oxygen exerted deteriorating effects on cells and induced cytochrome c (cyt-c) release from mitochondria. Although cyt-c generally induces apoptosis, singlet oxygen suppressed the apoptotic pathway. One reason is that singlet oxygen induced oxidation of cyt-c. Singlet oxygen also inactivated caspases and cathepsins that are involved in the apoptotic pathway.Although markers for oxidative modification of DNA and lipids are established, there is no proper marker for that of proteins. We examined oxidative products of four amino acids, Trp His, Met, and Tyr, N-formylkynurenine (NFK) was generated from Trp as a major product. Antibody against NFK was raised and found to be reactive to kynurenine, an intermediate of Trp oxidation, as well as NFK. This is the first report on the anti-NFK antibody and could be useful for detection of Trp oxidation and kynurenine in situ. We also established a system to detect glutathionylated proteins using biotinylated GST as a probe, which enabled simple detection of glutathionylated proteins.We found splenomegaly and anemia in SOD1-deficient mice during long-time breeding of them. In addition, almost all mice developed inflammation in facial skin, which is markedly delayed by administration of an antioxidant, N-acetylcysteine. Primary culture of keratinocytes isolated from neonatal mice indicated the decrease in adhering capability and requirement of serum component for culture. Although only minor phenotype has been reported for SOD1-deficient mice under conventional breeding conditions, long-term observation let us know that SOD1-deficient mice actually show symptoms that are similar to aged mice.
单线态氧对细胞产生恶化作用,并诱导线粒体释放细胞色素c(Cyt-c)。虽然Cyt-c一般诱导细胞凋亡,但单线态氧抑制了这一凋亡途径。一个原因是单线态氧诱导了Cyt-c的氧化。单线态氧还会使参与细胞凋亡的半胱氨酸酶和组织蛋白失活。虽然已经建立了DNA和脂质氧化修饰的标记物,但还没有合适的标记物来修饰蛋白质。我们研究了色氨酸、组氨酸、蛋氨酸和酪氨酸四种氨基酸的氧化产物,其中N-甲酰基犬尿氨酸(NFK)是由色氨酸生成的主要产物。抗NFK抗体被提出,并被发现与色氨酸氧化的中间产物Kynurenine和NFK反应。这是首次报道抗NFK抗体,可用于Trp氧化和犬尿氨酸的原位检测。我们还建立了以生物素化GST为探针的谷胱甘肽基化蛋白检测系统,使谷胱甘肽基化蛋白的检测变得简单。我们在长期饲养SOD1缺陷小鼠的过程中发现了脾肿大和贫血。此外,几乎所有小鼠的面部皮肤都出现了炎症,给予抗氧化剂N-乙酰半胱氨酸后,炎症明显延缓。原代培养的新生小鼠角质形成细胞的黏附能力和对培养所需血清成分的需求均下降。虽然在常规饲养条件下,SOD1基因缺陷小鼠只有很少的表型,但长期的观察让我们知道,SOD1基因缺陷小鼠实际上表现出与老龄小鼠相似的症状。

项目成果

期刊论文数量(54)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Abortive apoptotic pathway by singlet oxygen due to the suppression of caspase activation
由于抑制 caspase 激活而导致单线态氧导致的细胞凋亡途径失败
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Hamada;J. et al.;H.Taniguchi et al.;J.Hamada et al.;奥村宣明;Hideyo Sato;奥村宣明;Hideyo Sato;Daisuke Suto;Kaoru Otsu
  • 通讯作者:
    Kaoru Otsu
Dysregulation of TGF-β receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice.
TGF-β受体激活失调会导致核心岩藻糖缺陷小鼠的肺部发育异常和肺气肿样表型。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    N.Inaki;Y.Tsunezuka;K.Kawakami;H.Sato;T.Takino;M.Oda;G.Watanabe;Xiangchun Wang
  • 通讯作者:
    Xiangchun Wang
Medical Biochemistry 2^<nd> ed
医学生物化学 2^<nd> 版
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Niimi;A.;Junichi Fujii
  • 通讯作者:
    Junichi Fujii
Abortive apoptotic pathway by singlet oxygen due to the suppression of caspase activation.
由于半胱天冬酶激活的抑制,单线态氧导致细胞凋亡途径失败。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    奥村宣明;永井克也;T.Takino et al.;Yuki Nagaoka;Hideyo Sato;M.Ahmad et al.;Nakano N;Masaki Tanito;T.Takino et al.;Xiangchung Wang;Onishi Y;M.Ahmad et al.;Kaoru Otsu
  • 通讯作者:
    Kaoru Otsu
Cytoprotective Effects of Oral Geranylgeranylacetone, an Inducer of Thioredoxin and Heat Shock Protein 72, on Light-Induced Retinal Damage in Mice
口服香叶基香叶基丙酮(硫氧还蛋白和热休克蛋白 72 的诱导剂)对小鼠光诱导视网膜损伤的细胞保护作用
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Mitsushita;J.;Lambeth;D.;Kamata;T.;M Tanito
  • 通讯作者:
    M Tanito
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FUJII Junichi其他文献

FUJII Junichi的其他文献

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{{ truncateString('FUJII Junichi', 18)}}的其他基金

Redox regulation of endoplasmic reticulum stress and signals by reactive oxygen species
活性氧对内质网应激和信号的氧化还原调节
  • 批准号:
    22590262
  • 财政年份:
    2010
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Developments and Applications for Operator Inequalities
算子不等式的发展和应用
  • 批准号:
    20540166
  • 财政年份:
    2008
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation on a role of Prx4 in spermatogenesis by gene knockout approach
通过基因敲除方法研究 Prx4 在精子发生中的作用
  • 批准号:
    13670111
  • 财政年份:
    2001
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Remodeling of regulation of cell growth by glycogene and its application.
糖原对细胞生长调控的重塑及其应用。
  • 批准号:
    09680590
  • 财政年份:
    1997
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a method for early diagnosis of diabetic of complications by detecting glycation reaction intermediates.
开发通过检测糖化反应中间体来早期诊断糖尿病并发症的方法。
  • 批准号:
    09557017
  • 财政年份:
    1997
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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Trimethoprim: an overlooked contributor of trimethoprim-sulfamethoxazole idiosyncratic adverse drug reactions
甲氧苄啶:甲氧苄啶-磺胺甲恶唑异质药物不良反应的一个被忽视的因素
  • 批准号:
    10425272
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项目1:化学糜烂介导的皮肤损伤的新型药理抑制剂
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    10249113
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    2018
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Trimethoprim: an overlooked contributor of trimethoprim-sulfamethoxazole idiosyncratic adverse drug reactions
甲氧苄啶:甲氧苄啶-磺胺甲恶唑异质药物不良反应的一个被忽视的因素
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    10183270
  • 财政年份:
    2018
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    $ 2.3万
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Glutathione and redox state of progesterone-regulated breast cancer stem cells
黄体酮调节的乳腺癌干细胞的谷胱甘肽和氧化还原状态
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    9258301
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    2017
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阻止砷引起的皮肤损伤
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Blocking Arsenicals-induced Cutaneous Injury
阻止砷引起的皮肤损伤
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    9318612
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    2015
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    $ 2.3万
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Blocking Arsenicals-induced Cutaneous Injury
阻止砷引起的皮肤损伤
  • 批准号:
    8928418
  • 财政年份:
    2015
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Topical Silver Sulfadiazine Formulations with Improved Efficacy and Toxicity Prof
具有改善功效和毒性的局部磺胺嘧啶银制剂教授
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    8781304
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    2014
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Therapeutic Intervention of Lewisite-Mediated Cutaneous Blistering-Inflammation
路易氏剂介导的皮肤起泡炎症的治疗干预
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    8927140
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    2014
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    $ 2.3万
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Mouse models for esophageal Cox-2 oxidative stress and DNA damage
食管 Cox-2 氧化应激和 DNA 损伤的小鼠模型
  • 批准号:
    8680384
  • 财政年份:
    2013
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    $ 2.3万
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