Establishment of aspiration biopsy nucleic acid diagnosis of malignant lymphoma

恶性淋巴瘤抽吸活检核酸诊断的建立

• 批准号: 16590456
• 负责人: TAKANO Toru
• 金额: $ 2.24万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590456/
• 关键词: malignant lymphoma; Hashimoto's thyroiditis; vectorette PCR; immunoglobulin heavy chain; fine needle aspiration biopsy; nucleic acid diagnosis; Southern blot; cytology; イムノグロブリン; モノクロナリテイー

Distinguishing between thyroid malignant lymphoma and lymphocytic thyroiditis (Hashimoto's thyroiditis) is quite difficult and problematic. B-cell lymphomas display clonal immunoglobulin heavy chain (IgH) gene rearrangement, and Southern blot hybridization (SBH) is often used for detection of the monoclonality of the IgH gene. However, SBH is often problematic, since it requires a large volume of samples. Vectorette PCR is a method for amplifying DNA fragments of which the 5' sequences are unknown, using a modified linker to facilitate specific amplification of the targeted sequence, and it requires only 200 ng of genomic DNA to start with, which is easily obtainable by fine needle aspiration biopsy. We examined the efficiency in detecting the monoclonality of IgH gene in thyroid malignant lymphomas by vectorette PCR, which we started with 200 ng of genomic DNA obtained either from tissue samples or tumor aspirates. In the analysis using tissue samples, monoclonality was detected in 36 (76.6%) of 47 malignant lymphomas, whereas it was not detected in 10 samples of tissue of Hashimoto's thyroiditis. The sensitivity was almost the same as that with SBH in which monoclonality was detected in 33 (73.3%) of 45 malignant lymphomas. Further, one case in which aspirated tumor cells showed monoclonality in the IgH gene by vectorette PCR revealed to be diffuse large cell B-cell lymphoma by histopathological diagnosed after open biopsy. These results suggest that vectorette PCR may be a substitute for SBH, and since it requires only a small volume of samples, it is also used in the analysis of aspiration biopsies.

区分甲状腺恶性淋巴瘤和淋巴细胞性甲状腺炎（桥本甲状腺炎）是相当困难和有问题的。b细胞淋巴瘤表现为克隆性免疫球蛋白重链（IgH）基因重排，而Southern blot杂交（SBH）常用于检测IgH基因的单克隆性。然而，SBH常常存在问题，因为它需要大量的样本。Vectorette PCR是一种扩增未知5'序列的DNA片段的方法，使用修饰的连接体促进目标序列的特异性扩增，它只需要200ng的基因组DNA开始，这很容易通过细针穿刺活检获得。我们检验了用载体PCR检测甲状腺恶性淋巴瘤中IgH基因单克隆的效率，我们从组织样本或肿瘤抽吸物中获得200 ng基因组DNA。在组织样本分析中，47例恶性淋巴瘤中有36例（76.6%）检测到单克隆，而10例桥本甲状腺炎组织样本中未检测到单克隆。在45例恶性淋巴瘤中，有33例（73.3%）检测到单克隆，其敏感性与SBH几乎相同。此外，1例抽吸的肿瘤细胞通过载体PCR显示IgH基因单克隆，经开放活检组织病理学诊断为弥漫性大细胞b细胞淋巴瘤。这些结果表明，载体PCR可能是SBH的替代品，而且由于它只需要少量的样本，它也被用于穿刺活检的分析。

项目成果

Thrombospondin 1 mRNA as a candidate marker to detect thyroid-derived fibroblasts in fine needle aspiration biopsy of the thyroid.

血小板反应蛋白 1 mRNA 作为候选标记物，用于在甲状腺细针抽吸活检中检测甲状腺源性成纤维细胞。

• 期刊: Endocr J (印刷中)
• 作者: Takano T;et al.
• 通讯作者: et al.

Tensin3 is a novel thyroid-specific gene

• DOI: 10.1677/jme.1.01913
• 发表时间: 2006-02-01
• 期刊: JOURNAL OF MOLECULAR ENDOCRINOLOGY
• 影响因子: 3.5
• 作者: Maeda, I;Takano, T;Miyauchi, A
• 通讯作者: Miyauchi, A

Fetal cell carcinogenesis of the thyroid : a hypothesis for better understanding of gene expression profile and genomic alternation in thyroid carcinoma

甲状腺胎儿细胞癌变：更好地理解甲状腺癌基因表达谱和基因组改变的假设

• 发表时间: 2004
• 期刊: Endocr J 51(16)
• 作者: Yoshikawa M;Soda H;et al.;Takano T
• 通讯作者: Takano T

再構成された遺伝子の単クローン性を判定する方法

如何确定重排基因的单克隆性

• 发表时间: 2004

Differentiation of follicular thyroid adenoma from carcinoma by means of gene expression profiling with adapter-tagged competitive polymerase chain reaction.

通过接头标记的竞争性聚合酶链反应进行基因表达谱分析，区分滤泡性甲状腺腺瘤和癌。

• 发表时间: 2005
• 期刊: Oncology 69
• 作者: Taniguchi;K.;Takano;T.;Miyauchi;A.;Koizumi;K.;Ito;Y.;Takamura;Y.;Ishitobi;M.;Miyoshi;Y.;Taguchi;T.;Tamaki;Y.;Kato;K.;Noguchi;S.
• 通讯作者: S.