Development ofAspiration Biopsy Nucleic Acid Diagnosis of thyroid follicular carcinoma

甲状腺滤泡癌穿刺活检核酸诊断的进展

• 批准号: 18590531
• 负责人: TAKANO Toru
• 金额: $ 2.61万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590531/
• 关键词: thyroid; follicular carcinoma; TFF3; aspiration biopsy; diagnosis

The relative expression level of trefoil factor 3 (TFF3) mRNA to galectin-3 (LGALS3) mRNA (T/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T/G ratio in aspirates is difficult. We tested two methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber-EP4 antibody and the other was filtration through a nylon mesh. After selection with Ber-EP4 antibody, T/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T/G ratios between the aspirates and the corresponding tissues were less than 200% in all eight cases. Mesh filtration of aspirates proved superior results for the measurement of T/G ratio.

三叶因子3（TFF 3）mRNA与半乳糖凝集素3（LGALS 3）mRNA的相对表达水平（T/G比值）是鉴别甲状腺滤泡癌和腺瘤的有用指标。然而，由于同时抽吸的外周血细胞或浸润的淋巴细胞的干扰，抽吸物中的T/G比的精确测量是困难的。我们测试了两种选择甲状腺肿瘤细胞和从抽吸物中去除血细胞的方法。一种方法是用Ber-EP 4抗体包被的磁珠进行筛选，另一种方法是通过尼龙网过滤。经Ber-EP 4抗体筛选后，吸出物中T/G比值与相应组织中T/G比值有不同程度的差异。滤网过滤后，所有8例病例中抽吸物和相应组织之间的T/G比值差异均小于200%。抽吸物的筛网过滤证明了T/G比测量的上级结果。

项目成果

Fetal cell carcinogenesis : theory and practice

胎儿细胞癌变：理论与实践

• 发表时间: 2007
• 作者: Toru;Takano
• 通讯作者: Takano

穿刺吸引核酸診断法(VR法)による甲状腺悪性リンパ腫の鑑別診断-12例での実施経験の報告

细针穿刺核酸诊断法（VR法）鉴别诊断甲状腺恶性淋巴瘤12例实施经验报告

• 发表时间: 2007
• 作者: Toru;Takano;高野 徹
• 通讯作者: 高野 徹

Expression of oncofetal fibronectin mRNA in thyroid anaplastic carcinoma.

甲状腺未分化癌中癌胚纤连蛋白 mRNA 的表达。

• 发表时间: 2007
• 期刊: Jpn J Clin Oncol 37
• 作者: Takano T;et. al.
• 通讯作者: et. al.

Thrombospondin 1 mRNA as a candidate for a marker to detect thyroid-derived fibroblasts in fine needle aspiration biopsy of the thyroid

血小板反应蛋白 1 mRNA 作为候选标记物，用于在甲状腺细针抽吸活检中检测甲状腺源性成纤维细胞

• 发表时间: 2006
• 期刊: Endocr J 53
• 作者: Takano;T;et. al.
• 通讯作者: et. al.

ABND in thyroid malignant B-cell lymphoma

甲状腺恶性 B 细胞淋巴瘤中的 ABND

• 发表时间: 2006
• 作者: Toru;Takano
• 通讯作者: Takano