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Research on carcinogenesis modifying factor by static magnetic field exposure

静磁场暴露致癌调节因子的研究

基本信息

批准号：
16590486
负责人：
SUZUKI Yuji
金额：
$ 2.24万
依托单位：
Jikei University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

We have previously found that exposure to static magnetic fields (SMFs) increase the frequency of micronuclei. In this experiment, it became clear that co-exposure to SMFs increases the frequency of micronuclei induction by several mutagens including active oxygen radical inducible chemicals (doxorubicin (DOX), mitomycin C (MMC)). Therefore, the increase in micronucleus formation by SMFs might be related to active oxygen radical inducibility. In the present study, we used the mouse bone marrow micronucleus test to investigate whether antioxidant (ascorbic acid (AS)) can control the effects of SMF co-exposure on the frequency of micronuclei produced by radical-inducing chemicals. As a result, the following were clarified.1)As itself did not induce any micronuclei.2)Frequency of micronuclei by DOX or MMC increased by exposure to 5 T SMFs.3)Frequency of micronuclei induced by both DOX and MMC was increased by co-exposure to SMFs, but these increases were blocked by pretreatment with ascorbic acid.4)Frequency of micronuclei induced by co-exposure to MMC and SMFs decreased strongly at 20 min interval of ascorbic acid pre-treatment.5)Effect of ascorbic acid on the micronucleus frequency was maintained for at least 72 hours.6)Increased levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in bone marrow cells was observed after exposure to SMFs.These results suggest that SMFs exposure triggers the induction of mutagen-related radicals. This effect might increase the micronucleus frequency.

我们之前发现，暴露于静磁场 (SMF) 会增加微核的频率。在该实验中，很明显，共同暴露于 SMF 会增加几种诱变剂诱导微核的频率，包括活性氧自由基诱导化学物质（阿霉素 (DOX)、丝裂霉素 C (MMC)）。因此，SMF 增加微核形成可能与活性氧自由基诱导能力有关。在本研究中，我们使用小鼠骨髓微核测试来研究抗氧化剂（抗坏血酸（AS））是否可以控制 SMF 共同暴露对自由基诱导化学物质产生微核频率的影响。结果，澄清了以下内容。1) 由于其本身不诱导任何微核。2) 通过暴露于 5 T SMF，DOX 或 MMC 诱导的微核频率增加。3) 通过共同暴露于 SMF，DOX 和 MMC 诱导的微核频率增加，但这些增加被抗坏血酸预处理所阻止。4) 通过共同暴露于 SMF，诱导的微核频率增加。抗坏血酸预处理间隔 20 分钟后，MMC 和 SMF 强烈下降。5) 抗坏血酸对微核频率的影响维持至少 72 小时。6) 暴露于 SMF 后观察到骨髓细胞中 8-羟基-2'-脱氧鸟苷 (8-OHdG) 水平增加。这些结果表明，SMF 暴露触发了诱导与诱变剂相关的自由基。这种效应可能会增加微核频率。