Study on analytical toxicologic evaluation of endogenous γ-hydroxybutyric acid levels in postmortem body fluids

死后体液中内源性γ-羟基丁酸水平的毒理学分析评价研究

基本信息

批准号：
16590541
负责人：
MORIYA Fumio
金额：
$ 1.34万
依托单位：
Kochi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590541/
关键词：
Forensic Toxicology Endogenous γ-hydroxybutyric acid Postmortem production Smoking Drugs of abuse Urine Blood Gas chromatography 飲酒薬物濫用 γ-ヒドロキシ酪酸

项目摘要

Endogenous γ-hydroxybutyric acid (GHB) levels in various postmortem fluid samples of 25 autopsy cases with no or little putrefaction were measured by headspace gas chromatography after GHB was converted to γ-butyrolactone. Endogenous GHB levels were significantly higher in femoral venous blood (4.6±3.4μg/ml, n=23) than in cerebrospinal fluid (1.8±1.5μg/ml, n=9), vitreous humor (0.9±1.7μg/ml, n=8), bile (1.0±1.1μg/ml, n=9) and urine (0.6±1.2μg/ml, n=12). GHB levels were similar in blood samples taken from different sites. Cut-off limits of 30 and 10μg/ml can be proposed for blood and urine, respectively, to discriminate between exogenous and endogenous GHB in decedents showing no or little putrefaction.We have devised a simple method for detecting GHB in blood and urine by gas chromatography and liquid-liquid extraction with a back-extraction step for purification. The sensitivity of this method was approximately three times as high as that of the headspace gas chromatographic method.Endogenous levels of GHB in urine samples obtained from 20 healthy volunteers were measured by gas chromatography combined with liquid-liquid extraction. There were 4 average drinkers (males), 4 average smokers (males), and 12 nonsmokers/nondrinkers (7 males and 5 females). The urinary levels of GHB in males were higher in smokers (0.52±0.37μg/ml) than in nonsmokers/nondrinkers (0.28±0.21μg/ml) and drinkers (0.23±0.04μg/ml). No gender differences were observed in the urinary levels of endogenous GHB.When 2 g/kg of ethanol, 3 mg/kg of nicotine, 5 mg/kg of methamphetamine hydrochloride, 5 mg/kg of cocaine hydrochloride, 5 mg/kg of morphine hydrochloride, or 5 mg/kg of sodium amobarbital was subcutaneously administered to rabbits once a day for 5 days, no changes in endogenous GHB levels in blood were observed. Short term administration of those drugs of abuse may not affect endogenous production of GHB.

股静脉血液（4.6±3.4μg/ml，n = 23）的内源性GHB水平明显高于脑脊液（1.8±1.5μg/ml，n = 9），玻璃体幽默，0.9±1.7μg/ml，n = 8），Bile（1.0±1.0±1.1μg/ml，n = 9 = 9）（0.6±1.2μg/ml，n = 12）。从不同地点采集的血液样本中GHB水平相似。可以分别提出30和10μg/ml的截止极限，以分别用于血液和尿液，以区分未表现出或很少腐烂的死者中的外源性和内源性GHB。我们已经设计了一种简单的方法，可以通过气相色谱和液体液化来检测血液和尿液中的GHB，并通过液体液体萃取，以纯化的纯化步骤。该方法的敏感性大约是顶空气相色谱法的三倍。通过气相色谱和液态萃取的气相色谱法测量了从20位健康志愿者获得的尿液样品中的GHB水平。有4个平均饮酒者（男性），4名普通吸烟者（男性），还有12名非吸烟者/非养殖者（7名男性和5名女性）。吸烟者（0.52±0.37μg/ml）中GHB的尿液水平高于非吸烟者/非甘油酸（0.28±0.21μg/ml）和饮酒者（0.23±0.04μg/ml）。在内源性GHB的尿液水平上未观察到性别差异。当2 g/kg乙醇，3 mg/kg尼古丁，5 mg/kg盐酸甲基苯丙胺，5 mg/kg可卡因可卡因盐酸，盐酸盐，5 mg/kg的5 mg/kg/kg/kg/kg/kg/kg/kg/kg的5 mg/kg每天对兔子的皮下给药5天，没有观察到血液中内源性GHB水平的变化。这些滥用药物的短期服用可能不会影响GHB的内源性产生。

项目成果

期刊论文数量（14）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Site-dependent production of γ-hydroxybutyric acid in the early post-mortem period

尸检早期γ-羟基丁酸的位点依赖性产生

DOI：
发表时间：
2005
期刊：
Forensic Science International Vol.148-No.2-3
影响因子：
0
作者：
I.Yuasa;K.Umetsu;S.Tuchida;Y.Irizawa;L.Henke;J.Henke;Yoshmura N.;Fumio Moriya
通讯作者：
Fumio Moriya

Site-dependent production of γ-hydroxybutyric acid in the early postmortem period

死后早期γ-羟基丁酸的位点依赖性产生