Solid-phase extraction and analysis of twenty antidepressant drugs in human plasma by LC/MS with SSI method

建立了超声喷雾电离（SSI）液相色谱/质谱法同时测定人血浆中20种抗抑郁药物（丙咪嗪、阿米替林、地西帕明、三氯丙帕明、去甲替林、氯丙帕明、阿莫沙平、洛非帕明、多舒乐平、马普替林、米安色林、塞替林、曲唑酮、氟伏沙明、帕罗西汀、米那西普兰、舒必利、坦旋酮、哌醋甲酯和美利曲辛）的方法。色谱柱为Inertsil C-8，流动相为甲醇：10mM乙酸铵（pH 5.0）：乙腈（70:20:10），流速为0.10 mL/min，温度为35℃。将这些药物添加到人血浆中的固相萃取用Oasis^< <〇！> HLB筒柱。加样回收率为69 ~ 102%，检出限为0.03 ~ 0.63 μg/mL。本程序为抗抑郁药物的筛选提供了一种更简单、更方便的方法，并将对法医毒理学调查有用。

A simultaneous determination of twenty antidepressant drugs (imipramine, amitriptyline, desipramine, trimipramine, nortriptyline, clomipramine, amoxapine, lofepramine, dosulepin, maprotiline, mianserin, setiptiline, trazodone, fluvoxamine, paroxetine, milnacipran, sulpiride, tandspirone, methylphenidate and melitracen) in human plasma was developed using LC/MS with sonic spray ionization (SSI) method. These drugs showed good separation and sensitivity by LC-MS using an Inertsil C-8 column with methanol : 10mM ammonium acetate (pH 5.0) : acetonitrile (70:20:10) as mobile phase at 0.10 mL/min at 35℃. Solid-phase extraction of these drugs added to the human plasma was performed with an Oasis^<【○!R】> HLB cartridge column. Recovery and limit of detection of these drugs were between 69 and 102% and between 0.03 and 0.63 μg/mL, respectively. The present procedure offers an easier and more convenient screening method for antidepressants, and will be useful for forensic toxicology investigations.