Liquid Chromatography-Tandem Mass Spectropmetry (LC-MS/MS)

液相色谱-串联质谱 (LC-MS/MS)

• 批准号: 524864207
• 金额: --
• 依托单位: Technische Universität Dortmund
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/524864207?language=en
• 关键词: Liquid; Chromatography; Tandem; Mass; Spectropmetry

The research underlying the projects described covers a wide range of quantitative and qualitative research questions related to the analysis of modified and unmodified peptides. A further focus is the time-resolved detection of small molecule metabolites. Both classes of analytes are predestined for targeted mass spectrometric studies and for their unequivocal detection, quantification and publication-relevant characterization, liquid chromatography coupled to tandem mass spectrometry based on a triple quadrupole configuration is currently the gold standard due to ist excellent sensitivity. The technology allows the highly specific, parallel quantification of target molecules in complex reaction mixtures over a dynamic range of up to more than six orders of magnitude from the femto- to the nanogram range. It is in particular unrivalled for kinetic measurements such as time resolved expression analyses of complex protein samples or post-translational modifications. The optional coupling of the mass spectrometer unit to an ultra-high-performance liquid chromatography system or to a nano-liquid chromatography system allows the use of the mass spectrometer for project-related routine measurements as well as for more specific analyses of samples from limited biological source material. A triple quadrupole mass spectrometer coupled with liquid chromatography is currently not available at the Department of Chemistry and Chemical Biology at TU Dortmund University and, due to ist broad applicability in various areas of biochemistry, chemical biology and organic chemistry, represents an imperative addition to the analytical instrumentation available at the site

所述项目的基础研究涵盖了与分析修饰和未修饰多肽有关的广泛的定量和定性研究问题。另一个焦点是小分子代谢物的时间分辨检测。这两类分析物都注定要用于靶向质谱学研究，并由于其明确的检测、定量和与出版物相关的表征，基于三重四极杆构型的液相色谱与串联质谱仪联用，由于其出色的灵敏度，目前是金标准。该技术允许在高达六个数量级的动态范围内对复杂反应混合物中的目标分子进行高度特定的并行量化，从毫微克到纳克范围。它在动力学测量方面尤其无与伦比，例如复杂蛋白质样本的时间分辨表达分析或翻译后修饰。质谱仪单元与超高性能液相色谱系统或纳米液相色谱系统的可选耦合允许将质谱仪用于与项目相关的常规测量以及对来自有限生物来源材料的样品进行更具体的分析。TU Dortmund大学化学和化学生物学系目前还不能使用三重四极杆质谱计和液相色谱仪，并且由于在生物化学、化学生物学和有机化学的各个领域具有广泛的适用性，这是对现场现有分析仪器的必要补充。