Liquid Chromatography-Tandem Mass Spectropmetry (LC-MS/MS)

所描述的项目的基础研究涵盖了与修饰和未修饰肽分析相关的广泛的定量和定性研究问题。另一个焦点是小分子代谢物的时间分辨检测。这两类分析物都注定用于靶向质谱研究，并且对于它们的明确检测、定量和出版物相关表征，基于三重四极杆配置的液相色谱-串联质谱法由于其优异的灵敏度而成为目前的金标准。该技术允许在从毫微微到纳克范围的高达六个数量级以上的动态范围内对复杂反应混合物中的目标分子进行高度特异性的并行定量。特别是对于动力学测量，如复杂蛋白质样品或翻译后修饰的时间分辨表达分析，它是无与伦比的。质谱仪单元与超高效液相色谱系统或纳米液相色谱系统的可选耦合允许质谱仪用于项目相关的常规测量以及用于来自有限生物源材料的样品的更具体分析。多特蒙德大学化学与化学生物学系目前还没有一台三重四极杆质谱仪与液相色谱仪联用，由于其在生物化学、化学生物学和有机化学的各个领域具有广泛的适用性，因此是该研究中心现有分析仪器的必要补充

The research underlying the projects described covers a wide range of quantitative and qualitative research questions related to the analysis of modified and unmodified peptides. A further focus is the time-resolved detection of small molecule metabolites. Both classes of analytes are predestined for targeted mass spectrometric studies and for their unequivocal detection, quantification and publication-relevant characterization, liquid chromatography coupled to tandem mass spectrometry based on a triple quadrupole configuration is currently the gold standard due to ist excellent sensitivity. The technology allows the highly specific, parallel quantification of target molecules in complex reaction mixtures over a dynamic range of up to more than six orders of magnitude from the femto- to the nanogram range. It is in particular unrivalled for kinetic measurements such as time resolved expression analyses of complex protein samples or post-translational modifications. The optional coupling of the mass spectrometer unit to an ultra-high-performance liquid chromatography system or to a nano-liquid chromatography system allows the use of the mass spectrometer for project-related routine measurements as well as for more specific analyses of samples from limited biological source material. A triple quadrupole mass spectrometer coupled with liquid chromatography is currently not available at the Department of Chemistry and Chemical Biology at TU Dortmund University and, due to ist broad applicability in various areas of biochemistry, chemical biology and organic chemistry, represents an imperative addition to the analytical instrumentation available at the site