Development of New device for Regeneration of small intestinal tissue

1.开发用于再生小肠组织的创新聚乳酸管（或聚乙醇酸）。我们已经完成了创新的聚乳酸（或聚乙醇酸）管，可适用于小肠再生。我们还发现bFGF是小肠组织再生最有用的生长因子。因此，我们完成了生物可降解明胶微球，它可以持续释放bFGF。我们将这些微球附着在管内，并将骨髓细胞注入该管（我们将其命名为小肠再生管=SIR管）。将SIR管移植到切除小肠的大鼠小肠内进行实验。有趣的是，移植后2个月，SIR管内可见小肠组织再生.对再生小肠组织进行组织学和功能学检查。结果，微小的内窥镜检查结果显示，绒毛上皮在移植后一个月出现。此外，我们还研究了再生小肠中免疫调节细胞的比例。组织学和流式细胞仪分析显示，再生小胶质细胞与正常小胶质细胞相比，巨噬细胞、T、B细胞的比例无显著性差异.我们将GFP骨髓细胞注入SIR管中。移植后三个月，我们研究了GFP骨髓细胞在再生小肠中分化成什么样的细胞。结果，共聚焦显微镜结果显示，GFP阳性的肌成纤维细胞和小肠上皮细胞，这表明移植的骨髓细胞分化为上皮细胞和间充质细胞，并组成新的再生小肠。

1. Development of innovative polylactic acid tube (or polyglycolic acid) for regenerating small intestinal tissue. We have completed innovative polylactic acid (or polyglycolic acid) tube which could be suitable for regeneration of small intestine. We also showed that bFGF is most useful growth factor for regeneration of small intestinal tissue. Therefore, we completed the biodegradable gelatin microspheres, which can continuously release bFGF. We attached these microspheres to inside of the tube and injected bone marrow cells into this tube (we named it as small intestinal regenerating tube=SIR tube.). We transplanted SIR tube to small intestine of rat, which had taken resection of small intestine for experiment. Interestingly, regeneration of small intestinal tissue was observed inside of SIR tube 2 months after transplanting.2. We checked regenerated small intestinal tissue histologically and functionally. As a result, minute endoscopic findings revealed that villous epithelium was appeared one month after transplanting.3. Moreover, we investigated the proportion of immune-regulating cells in regenerated small intestine. Histological and FACS analysis revealed that there was no significant difference of the proportion of macrophages, T, and B cells in between regenerated and normal small intestine.4. We injected GFP bone marrow cells in SIR tube. Three months after transplanting this tube, we investigated what kinds of cell GFP bone marrow cells would differentiate into in regenerated small intestine. As a result, confocal microscopic findings showed that GFP was positive for myofibroblasts and small intestinal epithelium, which suggested that transplanted bone marrow cells differentiated into epithelium and mesenchymal cells and compose of newly regenerated small intestine.