THE ELECTRON MICROSCOPICAL ANALYSIS FOR CELL LINEAGE AND REPAIR FUNCTION OF BONE MARROW CELL IN CIRRHOSIS MICE AND NASH MICE

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We analyzed micro structure and characterization of infused GFP-positive BMC in CCl4 induced liver cirrhosis mice and NASH mice using Electron Microscopy (EM). Some GFP-positive cells were located in hepatic cord. These cells increased lysosome structure in cytoplasm.,Some cells co-expressed both GFP and Liv2 expression. All the Liv2 positive cells and A6 positive cells were circular forms and smaller than hepatocyte and located around hepatocytes in liver. We showed the Maid and MMP9 expression was increased with BMC infusion. Maid positive cells and MMP9 positive cells were also located at recipient liver and looked like round cell and were similar to hepatocytes in both size and feature. We analyzed GFP positive BMC were two kind of cell groups. One group cells were similar to hepatocyte in size and located around fiber (MMP9, Maid,Liv8 positive cells). The other group cells were small size and located around hepatocytes (A6, Liv2 positive cells). In EM level, we confirmed that infused GFP positive BMC consisted of two groups and they had several character. They repopulated into hepatic cord and fibrosis area.