THE ELECTRON MICROSCOPICAL ANALYSIS FOR CELL LINEAGE AND REPAIR FUNCTION OF BONE MARROW CELL IN CIRRHOSIS MICE AND NASH MICE

肝硬化小鼠和纳什小鼠骨髓细胞谱系及修复功能的电子显微镜分析

• 批准号: 21790668
• 负责人: YAMAMOTO Naoki
• 金额: $ 2.75万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790668/
• 关键词: 移植・再生医療; 電子顕微鏡; 再生医学; 発生・分化; 肝線維化; 非アルコール性肝炎; 再生医療; 骨髄細胞; GFP/CC14モデル; 非アルコール性脂肪性肝炎; 骨髄幹細胞; 幹細胞; 肝硬変

We analyzed micro structure and characterization of infused GFP-positive BMC in CCl4 induced liver cirrhosis mice and NASH mice using Electron Microscopy (EM). Some GFP-positive cells were located in hepatic cord. These cells increased lysosome structure in cytoplasm.,Some cells co-expressed both GFP and Liv2 expression. All the Liv2 positive cells and A6 positive cells were circular forms and smaller than hepatocyte and located around hepatocytes in liver. We showed the Maid and MMP9 expression was increased with BMC infusion. Maid positive cells and MMP9 positive cells were also located at recipient liver and looked like round cell and were similar to hepatocytes in both size and feature. We analyzed GFP positive BMC were two kind of cell groups. One group cells were similar to hepatocyte in size and located around fiber (MMP9, Maid,Liv8 positive cells). The other group cells were small size and located around hepatocytes (A6, Liv2 positive cells). In EM level, we confirmed that infused GFP positive BMC consisted of two groups and they had several character. They repopulated into hepatic cord and fibrosis area.

我们使用电子显微镜（EM）分析了CCl4诱导的肝硬化小鼠和NASH小鼠中输注的GFP阳性BMC的显微结构和特征。部分GFP阳性细胞位于肝索内。这些细胞胞质内溶酶体结构增多，一些细胞共表达GFP和Liv2表达。所有Liv2阳性细胞和A6阳性细胞均呈圆形，体积小于肝细胞，分布于肝细胞周围。我们发现，随着BMC灌注，Maid和MMP9表达增加。Maid阳性细胞和MMP 9阳性细胞也位于受体肝内，呈圆形，大小和形态与肝细胞相似。GFP阳性BMC分为两类细胞。一组细胞大小与肝细胞相似，位于纤维周围（MMP 9、Maid、Liv8阳性细胞）。另一组细胞体积小，位于肝细胞周围（A6，Liv2阳性细胞）。在电镜水平上，我们证实输注GFP阳性BMC可分为两组，它们具有多种特征。它们在肝索和纤维化区重新定居。

项目成果

骨髄由来肝幹細胞の肝硬変修復時における様々な動態と微細構造解析

肝硬化修复过程中骨髓源性肝干细胞的各种动力学及超微结构分析

• 发表时间: 2010
• 作者: Marumoto Y;Terai S;Urata Y;Matsumoto T;Mizunaga Y;Yamamoto N;Jin H;Fujisawa K;Murata T;Shinoda K;Nishina H;Sakaida I.;Matsumoto T;山本直樹;Naoki Yamamoto;Naoki Yamamoto;山本直樹
• 通讯作者: 山本直樹

THE ELECTRON MICROSCOPICAL ANALYSIS FOR CELL LINEAGE OF BONE MARROW CELL DIFFERENTIATION IN CIRRHOSIS MICE

肝硬化小鼠骨髓细胞分化的电子显微镜分析

• 发表时间: 2010
• 作者: Marumoto Y;Terai S;Urata Y;Matsumoto T;Mizunaga Y;Yamamoto N;Jin H;Fujisawa K;Murata T;Shinoda K;Nishina H;Sakaida I.;Matsumoto T;山本直樹;Naoki Yamamoto
• 通讯作者: Naoki Yamamoto

骨髄中の肝幹細胞の動態と微細構造解析-GFP/CCl4モデル-

骨髓中肝干细胞的动力学和超微结构分析-GFP/CCl4模型-

• 发表时间: 2010
• 作者: Marumoto Y;Terai S;Urata Y;Matsumoto T;Mizunaga Y;Yamamoto N;Jin H;Fujisawa K;Murata T;Shinoda K;Nishina H;Sakaida I.;Matsumoto T;山本直樹;Naoki Yamamoto;Naoki Yamamoto;山本直樹;山本直樹
• 通讯作者: 山本直樹

免疫電顕を用いた骨髄由来細胞の肝硬変修復時の動態と各種特徴

免疫电镜观察肝硬化修复过程中骨髓来源细胞的动态及各种特征

• 发表时间: 2009
• 作者: Marumoto Y;Terai S;Urata Y;Matsumoto T;Mizunaga Y;Yamamoto N;Jin H;Fujisawa K;Murata T;Shinoda K;Nishina H;Sakaida I.;Matsumoto T;山本直樹;Naoki Yamamoto;Naoki Yamamoto;山本直樹;山本直樹;山本直樹;山本直樹;山本直樹;Naoki Yamamoto;Naoki Yamamoto;Naoki Yamamoto;Naoki Yamamoto;山本直樹;山本直樹
• 通讯作者: 山本直樹

Splenectomy reduces fibrosis and preneoplastic lesions with increased triglycerides and essential fatty acids in rat liver cirrhosis induced by a choline-deficient L-amino acid-defined diet

• DOI: 10.1111/j.1872-034x.2011.00784.x
• 发表时间: 2011-05-01
• 期刊: HEPATOLOGY RESEARCH
• 影响因子: 4.2
• 作者: Oishi, Toshiyuki;Terai, Shuji;Sakaida, Isao
• 通讯作者: Sakaida, Isao