One donor-multiple recipients islet allotransplantation using two-layer method

实验1：胰岛分离前用薄层法（TLM）保存胰岛，除了胰岛产量提高外，胰岛功能良好，几乎与新分离的胰岛相当。实验2：采用TLM -腺苷消化法，可以很好地维持消化后组织ATP水平。更多的胰岛可以被回收。新方法至少与传统方法分离的胰岛功能相当。实验3：在大鼠模型中，减压控制胶原酶注射（RPCI）在80 mmHg下可以促进持续高胰岛产量和体内功能。实验4：腹腔充氧PFC可改善门静脉内胰岛移植的预后

Exp 1 : Tew layer method (TLM) for pancreas preservation prior to islet isolation resulted in excellent islet function in addition to improved islet yield, which is almost comparable to freshly isolated islets.Exp 2 : Tissue ATP levels after digestion were well maintained using TLM with adenosine digestion method. Greater numbers of islets could be retrieved. The new method was at least equivalent to islet function isolated by conventional method.Exp 3 : Reduced-pressure controlled collagenase injection (RPCI) at 80 mmHg could contribute to consistently high islet yield and in vivo function in a rat model.Exp 4 : intraperitoneal oxygenated PFC improves the outcome of intraportal islet transplantation