Development of one-step protein expression and purification systemUsing silica particles

本研究的目的是利用二氧化硅诱导启动子和二氧化硅结合域建立新的蛋白表达和纯化系统。转录分析表明，胶体二氧化硅引起的铁缺乏激活了该启动子，铁摄取调节剂（Fur: TTHA0255）调节了该启动子。在大肠杆菌中发现了几乎相同的现象，我们已经成功地构建了硅诱导的表达载体。然而，一步表达和纯化的条件尚未完成。

The aim of this study is to develop novel protein expression and purification system using silica-inducible promoter and silica-binding domain. Transcriptional analysis revealed that iron deficient caused by colloidal silica activated this promoter and ferric uptake regulator (Fur: TTHA0255) regulated this promoter. Almost the same phenomenon was seen in E. coli, and we have succeeded in the construction of silica-inducible expression vector. However, conditions for one-step expression and purification have not completed.