A study for improvement of pancreatic islet transplantation outcome by using two-layer method and rare sugar

胰岛移植是治疗1型糖尿病的一种根治方法。胰岛分离后改善胰岛功能可改善胰岛移植的临床效果。在这项研究中，我们研究了在胰岛培养液中添加稀有糖D-异体糖是否对大鼠胰岛的存活率和功能有有益的影响。结果显示，在过夜培养中，加入D-异体糖组的胰岛素分泌量比不加D-异体糖组增加。此外，与D-Allse共培养后移植的糖尿病裸小鼠的糖尿病治愈率明显高于对照组。由于胰岛氧化应激标志物(丙二醛)通过与D-异丙糖共培养而显著降低，这种稀有的糖可能具有抗氧化作用。

Islet transplantation is a curative treatment option for Type-1 diabetes. Improving islet function after isolation would result in better clinical outcomes of islet transplantation. In this study, we examined whether addition of D-allose, one of rare sugars, to islet culture medium had a beneficial effect on rat islet viability and function. As results, insulin secretion increased in the group with D-allose in overnight culture compared to the control without D-allose. In addition, diabetic nude mice transplanted islets after co-culture with D-allose demonstrated significantly higher cure rate of diabetes compared to the control. Because a oxidative stress marker (malondialdehyde) of islets was significantly reduced by co-culture with D-allose, this rare sugar possibly has an antioxidative effect.