A study for improvement of pancreatic islet transplantation outcome by using two-layer method and rare sugar

双层法和稀有糖改善胰岛移植效果的研究

• 批准号: 23591866
• 负责人: SUZUKI Yasuyuki
• 金额: $ 3.33万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591866/
• 关键词: 希少糖; 膵島移植; 二層法; D-アロース

Islet transplantation is a curative treatment option for Type-1 diabetes. Improving islet function after isolation would result in better clinical outcomes of islet transplantation. In this study, we examined whether addition of D-allose, one of rare sugars, to islet culture medium had a beneficial effect on rat islet viability and function. As results, insulin secretion increased in the group with D-allose in overnight culture compared to the control without D-allose. In addition, diabetic nude mice transplanted islets after co-culture with D-allose demonstrated significantly higher cure rate of diabetes compared to the control. Because a oxidative stress marker (malondialdehyde) of islets was significantly reduced by co-culture with D-allose, this rare sugar possibly has an antioxidative effect.

胰岛移植是治疗1型糖尿病的一种有效方法。胰岛分离后胰岛功能的改善有助于改善胰岛移植的临床效果。在本研究中，我们研究了在胰岛培养基中添加一种罕见糖D-allose是否对大鼠胰岛的活力和功能有有益的影响。结果，与不含D-allose的对照组相比，隔夜培养中含有D-allose的组胰岛素分泌增加。此外，与D-allose共培养后移植胰岛的糖尿病裸鼠，其糖尿病治愈率明显高于对照组。由于胰岛的氧化应激标志物（丙二醛）与d -醛共培养显著降低，这种罕见的糖可能具有抗氧化作用。