A study for improvement of pancreatic islet transplantation outcome by using two-layer method and rare sugar

胰岛移植是1型糖尿病的治愈性治疗选择。分离后改善胰岛功能将导致胰岛移植的更好的临床结果。在这项研究中，我们研究了是否添加D-阿洛糖，一种罕见的糖，胰岛培养基中有一个有益的影响，大鼠胰岛的活力和功能。结果，与不含D-阿洛糖的对照组相比，在过夜培养中含D-阿洛糖的组中胰岛素分泌增加。此外，与对照组相比，糖尿病裸鼠移植胰岛与D-阿洛糖共培养后显示出显著更高的糖尿病治愈率。由于与D-阿洛糖共培养显著降低了胰岛的氧化应激标志物（丙二醛），因此这种稀有糖可能具有抗氧化作用。

Islet transplantation is a curative treatment option for Type-1 diabetes. Improving islet function after isolation would result in better clinical outcomes of islet transplantation. In this study, we examined whether addition of D-allose, one of rare sugars, to islet culture medium had a beneficial effect on rat islet viability and function. As results, insulin secretion increased in the group with D-allose in overnight culture compared to the control without D-allose. In addition, diabetic nude mice transplanted islets after co-culture with D-allose demonstrated significantly higher cure rate of diabetes compared to the control. Because a oxidative stress marker (malondialdehyde) of islets was significantly reduced by co-culture with D-allose, this rare sugar possibly has an antioxidative effect.