Mesenchymal stem cells from various mesenchymal tissues : Comparison of the properties and application for cartilage regeneration

来自不同间充质组织的间充质干细胞:软骨再生特性和应用的比较

基本信息

  • 批准号:
    16591478
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2005
  • 项目状态:
    已结题

项目摘要

Objective. To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue.Methods. Human mesenchymal tissues were harvested from 8 donors during the knee operations for the ligament injury. After collagenase digestion or gradient density separation, nucleated cells were plated at a proper density so that they expanded at maximum rate without colony-to-colony contact. Yield, expandability, differentiation potential, and epitope profile were compared among MSCs from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue.Results. Colony number per nucleated cells was lower and the cell number per colony was higher in the bone marrow than the other mesenchymal tissues. The cells were replated at low density every 14 days and bone marrow-, synovium-, and periosteum- derived cells retained their proliferation ability even at Passage 10. For chondrogenesis, the cells were pelleted and cultured in vitro, showing pellets from bone marrow-, synovium-, and periosteum- derived cells were larger and stained more extensively for cartilage matrix. Synovium-derived cells, in particular, had the greatest ability for chondrogenesis. For adipogenesis, Oil Red-O positive colony rate was higher in synovium- and adipose- derived cells. For osteogenesis, Alizarin Red positive colony rate was higher in bone marrow-, syovium-, and periosteum- derived cells. For epitope profile, 15 surface antigens were measured and the majority seemed similar irrespective of cell source.Conclusion. We revealed significant differences in the properties dependent on cell sources beyond donor and experimental variation. Superiority of synovium as cell source was demonstrated.
Objective.比较从骨髓、滑膜、骨膜、骨骼肌和脂肪组织中分离的人间充质干细胞(MSCs)的特性。在膝关节韧带损伤手术中,从8名供体中收获人间充质组织。在胶原酶消化或梯度密度分离后,将有核细胞以适当的密度铺板,使得它们以最大速率扩增而没有集落与集落接触。比较骨髓、滑膜、骨膜、骨骼肌和脂肪组织来源的MSCs的产量、扩增能力、分化潜能和表位特征。骨髓中每有核细胞的集落数较低,而每集落的细胞数高于其他间充质组织。每14天将细胞以低密度重新铺板,并且骨髓、滑膜和骨膜衍生的细胞甚至在第10代时仍保持其增殖能力。对于软骨形成,将细胞沉淀并在体外培养,显示来自骨髓、滑膜和骨膜来源的细胞的沉淀更大并且对软骨基质染色更广泛。特别是滑膜衍生的细胞,具有最大的软骨形成能力。对于脂肪形成,油红-O阳性集落率在滑膜和脂肪来源的细胞中较高。对于成骨,茜素红阳性集落率在骨髓、滑膜和骨膜来源的细胞中较高.对于表位谱,测量了15个表面抗原,并且大多数似乎相似,而不考虑细胞来源。我们揭示了显着差异的属性依赖于细胞来源以外的捐助者和实验的变化。证实滑膜作为细胞来源的优越性。

项目成果

期刊论文数量(38)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
In vitro chondrogenesis of human synovium-derived mesenchymal stem cells: Optimal condition and comparison with bone marrow-derived cells
  • DOI:
    10.1002/jcb.20546
  • 发表时间:
    2006-01-01
  • 期刊:
  • 影响因子:
    4
  • 作者:
    Shirasawa, S;Sekiya, I;Muneta, T
  • 通讯作者:
    Muneta, T
滑膜由来細胞は優れた増殖・軟骨分化能を有する-各種間葉細胞間の比較-
滑膜来源细胞具有优异的增殖能力和软骨分化能力 - 各种间充质细胞的比较 -
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    関矢一郎;坂口祐輔;柳下和慶;四官謙一;宗田大
  • 通讯作者:
    宗田大
Morphological examination during in vitro cartilage formation by human mesenchymal stem cells
  • DOI:
    10.1007/s00441-005-1140-6
  • 发表时间:
    2005-11-01
  • 期刊:
  • 影响因子:
    3.6
  • 作者:
    Ichinose, S;Tagami, M;Sekiya, I
  • 通讯作者:
    Sekiya, I
滑膜由来細胞・コラーゲンゲル複合体のin vitro軟骨分化の検討
滑膜来源细胞/胶原凝胶复合物的体外软骨分化检查
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    横山明子;関矢一郎;坂口祐輔;四宮謙一;秦 維郎;宗田 大
  • 通讯作者:
    宗田 大
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SEKIYA Ichiro其他文献

SEKIYA Ichiro的其他文献

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{{ truncateString('SEKIYA Ichiro', 18)}}的其他基金

Investigation of allogeneic synovial mesenchymal stem cells for cartilage and meniscus regeneration therapy
同种异体滑膜间充质干细胞用于软骨和半月板再生治疗的研究
  • 批准号:
    15H04953
  • 财政年份:
    2015
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Magnesium enhances adherence and cartilage formation of synovial mesenchymal stem cells through integrins
镁通过整合素增强滑膜间充质干细胞的粘附和软骨形成
  • 批准号:
    21591914
  • 财政年份:
    2009
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Enhancement of intraarticular tissue repairs by injecting a large number of synovial stem cells into the knee joint
通过向膝关节注射大量滑膜干细胞来增强关节内组织修复
  • 批准号:
    18591657
  • 财政年份:
    2006
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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基于甲状旁腺素重塑腱骨止点微结构及促软骨和抑瘢痕的机制研究
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