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Enhancement of intraarticular tissue repairs by injecting a large number of synovial stem cells into the knee joint

通过向膝关节注射大量滑膜干细胞来增强关节内组织修复

基本信息

批准号：
18591657
负责人：
SEKIYA Ichiro
金额：
$ 2.57万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

We investigated the role and effect of synovial stem cells in the knee joint.(1) We isolated rat mesenchymal stem cells (MSCs) from bone marrow, synovium, periosteum, adipose, muscle and compared their properties for yield, expansion, and multipotentiality. Synovial stem cells had the greatest potential for both proliferation and chondrogenesis.(2) We examined the features of adipose synovium-stem cells, fibrous synovium-stem cells, and subcutaneous fat-stem cells to determine their similarities. Adipose synovium-stem cells were more similar to fibrous synovium-derived cells than to subcutaneous fat-stem cells.(3) We transplanted undifferentiated synovial stem cells into a full-thickness articular cartilage defect of adult rabbits and defined the cellular events. Transplanted synovial stem cells were altered over a time course according to the microenvironments.(4) We examined the behavior and properties of MSCs derived from synovial fluid. Synovial fluid-MSCs were similar to synovium-MSCs, and increased in number after anterior cruciate ligament injury and surgery without marked alteration of the properties.(5) We investigated whether synovial fluid expanded synovial MSCs. Autologous synovial fluid enhanced expansion of MSCs in tissue culture of synovium from osteoarthritis patients by promoting cell migration.(6) We investigated the effect and mechanism of the implantation of the synovial MSCs on tendon-bone healing in rats. Implantation of synovial MSCs into bone tunnel accelerated early remodeling of tendon-bone healing.These results demonstrate that intraarticular injection of synovial MSCs can boost natural healing ability in the joint. Intraarticular injection of synovial stem cells is attractive because it requires no invasive procedure or artificial scaffold.

我们研究了滑膜干细胞在膝关节中的作用和作用。(1)我们从大鼠骨髓、滑膜、骨膜、脂肪和肌肉中分离了间充质干细胞（MSCs），并比较了它们的产量、扩增和多能性。滑膜干细胞在增殖和软骨形成方面具有最大的潜力。(2)我们研究了脂肪滑膜干细胞、纤维滑膜干细胞和皮下脂肪干细胞的特征，以确定它们的相似性。脂肪滑膜干细胞与纤维滑膜来源的细胞比与皮下脂肪干细胞更相似。(3)将未分化的滑膜干细胞移植到成年兔全层关节软骨缺损中，并确定细胞事件。移植的滑膜干细胞在一段时间内根据微环境发生改变。(4)我们研究了从滑液中提取的间充质干细胞的行为和性质。滑膜液-间充质干细胞与滑膜-间充质干细胞相似，在前交叉韧带损伤和手术后数量增加，但性质没有明显改变。(5)我们研究了滑膜液是否扩大了滑膜间充质干细胞。自体滑膜液通过促进细胞迁移增强骨关节炎患者滑膜组织培养中MSCs的扩增。(6)探讨滑膜间充质干细胞移植对大鼠肌腱骨愈合的影响及机制。滑膜间充质干细胞植入骨隧道加速肌腱骨愈合的早期重塑。这些结果表明关节内注射滑膜间充质干细胞可以提高关节的自然愈合能力。关节内注射滑膜干细胞是有吸引力的，因为它不需要侵入性手术或人工支架。