权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

To prevent Pseudomonas aeruginosa biofilms in the urinary tract -the search for antibiofilm agents-

为了预防尿路中的铜绿假单胞菌生物膜 - 寻找抗生物膜药物 -

基本信息

批准号：
16591597
负责人：
MONDEN Koichi
金额：
$ 2.3万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591597/
关键词：
Pseudomonas aeruginosa biofilm urinary tract infection antibiofilm agents experimental model indwelling catheter fever multidrug resistance green fluorescent protein レボフロキサシンホスホマイシン

项目摘要

In efforts to search for antibiofilm agents, we use experimental models for complicated urinary tract infections (UTI). We have used in vitro (a modified Robbins device and a capillary biofilm system) and in vivo (rat) models. In these models, a capillary biofilm system recently developed is a better in vitro model for evaluating antibiofilm agents. Pseudomonas aeruginosa OP14-210 isolated from a patient with a catheter-associated UTI was used. The biofilms were grown in glass capillary tubes under continuous flow conditions with artificial urine, and observed by confocal laser scanning microscopy. The potential antibiofilm agents were evaluated. The combination treatment of fosfomycin and fluoroquinolones against P.aeruginosa biofilms was effective and would appear beneficial against biofilm-related infections. This synergy obtained by these in vitro and in vivo models was confirmed at concentrations easily achievable in the urine of patients treated with clinical oral doses of these agents.We also investigated the relationships between biofilm-forming capacities of P.aeruginosa isolates and clinical background / antimicrobial resistance in UTI. The biofilm-forming capacities of P.aeruginosa isolates from catheter-related cases and / or febrile episodes were greater than those from catheter-unrelated cases and / or non-febrile episodes. The percentage of P.aeruginosa isolates exhibiting antimicrobial resistance to piperacillin, ceftazidime, imipenem/cilastatin, gentamicin and ciprofloxacin was higher among strong biofilm formers than among non-strong biofilm formers. These results suggested that the persistence of P.aeruginosa in the urinary tract is due to biofilm formation exhibiting antimicrobial resistance as well as multiple resistance mechanism of P.aeruginosa itself.

在寻找抗感染膜制剂的努力中，我们使用了复杂性尿路感染（UTI）的实验模型。我们已经使用了体外（改良的罗宾斯装置和毛细血管生物膜系统）和体内（大鼠）模型。在这些模型中，最近开发的毛细生物膜系统是一个更好的体外模型，用于评估生物膜剂。使用从导管相关UTI患者中分离的铜绿假单胞菌OP 14 -210。生物膜生长在玻璃毛细管连续流动条件下与人工尿液，并通过共聚焦激光扫描显微镜观察。评价了潜在的成膜剂。磷霉素和氟喹诺酮类药物联合治疗铜绿假单胞菌生物膜是有效的，并且似乎对生物膜相关感染有益。通过这些体外和体内模型获得的这种协同作用在用临床口服剂量的这些药剂治疗的患者的尿液中容易达到的浓度下得到证实。我们还研究了铜绿假单胞菌分离株的生物膜形成能力与UTI的临床背景/抗菌药物耐药性之间的关系。来自导管相关病例和/或发热事件的铜绿假单胞菌分离株的生物被膜形成能力大于来自导管无关病例和/或非发热事件的铜绿假单胞菌分离株。铜绿假单胞菌分离株对哌拉西林、头孢他啶、亚胺培南/西司他丁、庆大霉素和环丙沙星的耐药性在强生物膜形成者中的百分比高于非强生物膜形成者。这些结果表明，铜绿假单胞菌在尿路中的持久存在是由于生物膜形成表现出抗菌素耐药性以及铜绿假单胞菌本身的多重耐药机制。

项目成果

期刊论文数量（27）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

キャピラリーフローセルシステムにおける緑膿菌性バイオフィルムに対する抗菌薬の有効性評価

毛细管流通池系统中抗菌药物对铜绿假单胞菌生物膜的疗效评价

DOI：
发表时间：
2006
期刊：
第40回緑膿菌感染症研究会講演記録 40
影响因子：
0
作者：
古谷泰久;荒木勇雄;田邊信明;武田正之;Wakita T;狩山玲子ら
通讯作者：
狩山玲子ら

Combination treatment with fosfomycin and prulifloxacin against Pseudomonas aeruginosa growing in a biofilm in rat urinary tract infection model.

磷霉素和普卢利沙星联合治疗大鼠尿路感染模型中生物膜中生长的铜绿假单胞菌。

DOI：
发表时间：
2004
期刊：
Bacterial Adherence ＆ Biofilm 18
影响因子：
0
作者：
荒木勇雄;三神裕紀;別府正典;武田正之;Shinya Uehara et al.;Hayashi T;荒木勇雄;Asanuma K;Takeshi Mikuniya et al.
通讯作者：
Takeshi Mikuniya et al.

Synergistic effect of fosfomycin and fluoroquinolones against Pseudonmonas aeruginosa growing in a biofilm

磷霉素和氟喹诺酮类药物对生物膜中铜绿假单胞菌的协同作用