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Effects of stress and portal circulation on metabolism of the hepatocytes during isolated liver perfusion in weanling swine.

断奶猪离体肝脏灌注过程中应激和门脉循环对肝细胞代谢的影响。

基本信息

批准号：
16591783
负责人：
SEO Takahiko
金额：
$ 2.18万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

The present study was designed to investigate effects of portal circulation on metabolism of the hepatocytes during isolated liver perfusion with double bypass using automatic blood pumps in weanling swine. Isolated liver perfusion was established with bypass between the hepatic vein and portal vein for up to 4 hours in 12 weanling Yorkshire swine with a mean body weight of 8.8 kg. The perfusion circuit was primed with autologous fresh blood and infused with glucose-free 1/2 saline solution at 15 ml/hour during liver perfusion. The animals were divided into 3 groups by means of portal bypass flow rates; G-10 : liver perfusion at a flow rate of 10 ml/min/kg, G-20 : at a flow rate of 20 ml/min/kg, G-30 : at a flow rate of 30 ml/min/kg.As results, total protein and albumin were gradually decreased with time and total protein in G-20 decreased significantly than in G-10. Blood glucose was significantly elevated after initiation of liver perfusion, and gradually decreased thereafter in three groups. Glucose values were lowest in G-10 among all groups. On the contrary, triglyceride was gradually increased with time, and its increase was significantly marked in G-10. Total cholesterol, free fatty acid, phospholipids, and β-lipoprotein were gradually decreased with time in all groups, however, those decreases were marked in G-20. Lipid peroxide was increased immediately after initiation of liver perfusion and its elevation was marked and gradually in G-20.Liver function tests during isolated liver perfusion by means of GOT, GPT, LDH demonstrated that mild liver dysfunction occurred in G-10, which was thought to be due to mild hepatocytes ischemia. All parameters in G-30 were not reliable because transfusion during experiments was necessary in order to keep bypass flow.In conclusion, changes in hepatic metabolism during isolated liver perfusion were thought to be milder in the group with less portal bypass flow.

本研究旨在探讨断奶仔猪在全自动血泵双旁路离体肝灌流过程中门静脉循环对肝细胞代谢的影响。12头平均体重8.8 kg的约克夏断奶仔猪，在肝静脉和门静脉之间转流4h，建立肝脏隔离灌流模型。灌流回路预注自体新鲜血液，在肝脏灌流过程中以15ml/h的速度注入无糖的1/2生理盐水。根据门静脉旁路血流速度将动物分为3组：G-10组：肝脏灌流速度为10ml/min/kg，G-20组：流量为20ml/min/kg，G-30组：流量为30ml/min/kg，总蛋白和白蛋白随时间逐渐减少，G-20组比G-10组明显减少。开始肝脏灌流后，三组大鼠血糖均显著升高，随后逐渐下降。G-10组的血糖值在所有组中最低。反之，甘油三酯随时间逐渐升高，以G-10组升高最为显著。随着时间的推移，总胆固醇、游离脂肪酸、磷脂和β-脂蛋白均逐渐降低，但在G-20组下降最为明显。GOT、GPT、LDH检测显示，G-10组出现轻度肝功能障碍，推测为轻度肝细胞缺血所致。G-30中的所有参数都不可靠，因为实验期间需要输液以保持旁路血流。总之，门静脉旁路血流较少的组在隔离肝脏灌流期间肝脏代谢的变化被认为是温和的。